Polystyrene Particles Reveal Pore Substructure As They Translocate

Pevarnik, Matthew; Healy, Ken; Toimil-Molares, María Eugenia; Morrison, Alan P.; Létant, Sonia E.; Siwy, Zuzanna S.

doi:10.1021/nn302413u

Cited by 67 publications

(137 citation statements)

References 44 publications

(90 reference statements)

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“…16,22,23 Figure 2 presents recordings performed with a pore that had an average opening diameter of 770 nm. Each type of particle was studied individually from a suspension in 0.1 M KCl, pH 8 in the presence of Tween 80.…”

Section: Resultsmentioning

confidence: 99%

“…The relative depth of a resistive pulse for a spherical particle can be predicted from the following equation: 6,9,22 ோ ିோ…”

Section: Resultsmentioning

confidence: 99%

“…21 As shown by our group before, the pores are characterized by significant longitudinal irregularities revealed through resistive-pulse experiments with polystyrene 4 beads as well as metal replicas of the pores. 16,22,23,24 The diameter undulations of PET pores are attributed to the laminar structure of the films, and penetration of the etchant between the strata. 25 Two types of silica rods 26 with diameters of ~220 nm and lengths of 590 and 1950 nm, respectively were detected as they electrokinetically passed through single pores with an average opening diameter of ~1 µm.…”

Section: Introductionmentioning

confidence: 99%

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Pores with Longitudinal Irregularities Distinguish Objects by Shape

et al. 2015

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The resistive-pulse technique has been used to detect and size objects which pass through a single pore. The amplitude of the ion current change observed when a particle is in the pore is correlated with the particle volume. Up to date, however, the resistive-pulse approach has not been able to distinguish between objects of similar volume but different shapes. In this manuscript, we propose using pores with longitudinal irregularities as a sensitive tool capable of distinguishing spherical and rod-shaped particles with different lengths. The ion current modulations within resulting resistive pulses carry information on the length of passing objects. The performed experiments also indicate the rods rotate while translocating, and displace an effective volume that is larger than their geometrical volume, and which also depends on the pore diameter.

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Section: Resultsmentioning

confidence: 99%

“…The relative depth of a resistive pulse for a spherical particle can be predicted from the following equation: 6,9,22 ோ ିோ…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Pores with Longitudinal Irregularities Distinguish Objects by Shape

et al. 2015

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“…13,16 Using this model (see SI for more details) we determined the radius r of each particle from the resistive pulse intensity Δ R it produced during the passage through the nanochannel. In Figure 4b, we compare diffusion coefficients D extracted from optical track analysis and the dimension of particles determined electrically.…”

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confidence: 99%

Simultaneous Electro-Optical Tracking for Nanoparticle Recognition and Counting

et al. 2015

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We present the first detailed experimental observation and analysis of nanoparticle electrophoresis through a nanochannel obtained with synchronous high-bandwidth electrical and camera recordings. Optically determined particle diffusion coefficients agree with values extracted from fitting electrical transport measurements to distributions from 1D Fokker–Planck diffusion-drift theory. This combined tracking strategy enables optical recognition and electrical characterization of nanoparticles in solution, which can have a broad range of applications in biology and materials science.

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“…8 Pulse shape is a convolution of pore and particle geometry, and pulse shape reflects pore topography. 9 Pulse width is the residence time within the pore, decreases with particle velocity, 10 and increases with particle-pore interactions, such as adsorption. 11 Particle trajectory through the pore also plays an important role in both pulse width and amplitude.…”

Section: Resistive-pulse Sensing With Out-of-plane Nanoporesmentioning

confidence: 99%

Conductivity-based detection techniques in nanofluidic devices

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Haywood

Kneller

et al. 2015

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This review covers conductivity detection in fabricated nanochannels and nanopores. Improvements in nanoscale sensing are a direct result of advances in fabrication techniques, which produce devices with channels and pores with reproducible dimensions and in a variety of materials. Analytes of interest are detected by measuring changes in conductance as the analyte accumulates in the channel or passes transiently through the pore. These detection methods take advantage of phenomena enhanced at the nanoscale, such as ion current rectification, surface conductance, and dimensions comparable to the analytes of interest. The end result is the development of sensing technologies for a broad range of analytes, e.g., ions, small molecules, proteins, nucleic acids, and particles.

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Polystyrene Particles Reveal Pore Substructure As They Translocate

Cited by 67 publications

References 44 publications

Pores with Longitudinal Irregularities Distinguish Objects by Shape

Pores with Longitudinal Irregularities Distinguish Objects by Shape

Simultaneous Electro-Optical Tracking for Nanoparticle Recognition and Counting

Conductivity-based detection techniques in nanofluidic devices

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