Polystyrene nanoparticles activate ion transport in human airway epithelial cells

McCarthy, Joanna; Gong, Xiaowen; Nahirney, Drew; Duszyk, Marek; Radomski, M. W.

doi:10.2147/ijn.s21145

Cited by 50 publications

(28 citation statements)

References 42 publications

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“…Observations of an interaction between polystyrene ENPs exposure and the activity of the ion channel in isolated epithelial cells suggests that there is a degree of interaction between the particle and components of these channels. Ultimately such effects may have implications for the homeostasis of relevant cell types (McCarthy et al 2011). Further these results, and also those discussed earlier in relation to Ag ENPs and Na ?…”

Section: Mechanisms Of Enp Assimilation Into Biological Systemssupporting

confidence: 76%

Analytical approaches to support current understanding of exposure, uptake and distributions of engineered nanoparticles by aquatic and terrestrial organisms

et al. 2014

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Initiatives to support the sustainable development of the nanotechnology sector have led to rapid growth in research on the environmental fate, hazards and risk of engineered nanoparticles (ENP). As the field has matured over the last 10 years, a detailed picture of the best methods to track potential forms of exposure, their uptake routes and best methods to identify and track internal fate and distributions following assimilation into organisms has begun to emerge. Here we summarise the current state of the field, focussing particularly on metal and metal oxide ENPs. Studies to date have shown that ENPs undergo a range of physical and chemical transformations in the environment to the extent that exposures to pristine well dispersed materials will occur only rarely in nature. Methods to track assimilation and internal distributions must, therefore, be capable of detecting these modified forms. The uptake mechanisms involved in ENP assimilation may include a range of trans-cellular trafficking and distribution pathways, which can be followed by passage to intracellular compartments. To trace toxicokinetics and distributions, analytical and imaging approaches are available to determine rates, states and forms. When used hierarchically, these tools can map ENP distributions to specific target organs, cell types and organelles, such as endosomes, caveolae and lysosomes and assess speciation states. The first decade of ENP ecotoxicology research, thus, points to an emerging paradigm where exposure is to transformed materials transported into tissues and cells via passive and active pathways within which they can be assimilated and therein identified using a tiered analytical and imaging approach.

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Section: Mechanisms Of Enp Assimilation Into Biological Systemssupporting

confidence: 76%

Analytical approaches to support current understanding of exposure, uptake and distributions of engineered nanoparticles by aquatic and terrestrial organisms

et al. 2014

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“…Interestingly, nanoparticles can directly interact with proteins and ion channels and these effects can also contribute to the profile of SiNP actions on platelets. 42,43 In conclusion, engineered amorphous SiNP penetrated the platelet plasma membrane and stimulated a rapid and prolonged NO release leading to eNOS uncoupling, ONOO -over-production and eventually, a low [NO]/[ONOO -] ratio and high nitroxidative/oxidative stress. The unfavorable situation, thus produced, favored receptors activation (GPIIb/IIIa) and expression (SELP) on the platelet surface membrane leading to platelet activation and aggregation.…”

Section: Discussionmentioning

confidence: 93%

Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis

Corbalan

Medina

Jacoby³

et al. 2012

IJN

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Methods: Nanoparticle-platelet interaction was examined using transmission electron microscopy. Electrochemical nanosensors were used to measure the levels of NO and ONOO -released by platelets upon nanoparticle stimulation. Platelet aggregation was studied using light aggregometry, flow cytometry, and phase contrast microscopy. Results: Amorphous SiNP induced NO release from platelets followed by a massive stimulation of ONOO -leading to an unfavorably low [NO]/[ONOO -] ratio. In addition, SiNP induced an upregulation of selectin P expression and glycoprotein IIb/IIIa activation on the platelet surface membrane, and led to platelet aggregation via adenosine diphosphate and matrix metalloproteinase 2-dependent mechanisms. Importantly, all the effects on platelet aggregation were inversely proportional to nanoparticle size. Conclusions: The exposure of platelets to amorphous SiNP induces a critically low [NO]/[ONOO -] ratio leading to platelet aggregation. These findings provide new insights into the pharmacological profile of SiNP in platelets.

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“…90 One important example in this context is the cystic fibrosis transmembrane conductance regulator (CFTR) that is activated by membrane stretch. 91 McCarthy et al 92 recently demonstrated that polystyrene NPs direct activation of CFTR channels in a monolayer of Calu-3, a human airway submucosal cell line, and completed these data also in baby hamster kidneys engineered to express the wild-type CFTR gene, thus confirming that NPs have the ability to act as modulators of ion-channel function in human airway epithelial cells.…”

mentioning

confidence: 81%

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

Panariti¹,

Miserocchi²,

Rivolta³

2012

NSA

160

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Nanoparticles (NPs) are materials with overall dimensions in the nanoscale range. They have unique physicochemical properties, and have emerged as important players in current research in modern medicine. In the last few decades, several types of NPs and microparticles have been synthesized and proposed for use as contrast agents for diagnostics and imaging and for drug delivery; for example, in cancer therapy. Yet specific targeting that will improve their delivery still represents an unsolved challenge. The mechanism by which NPs enter the cell has important implications not only for their fate but also for their impact on biological systems. Several papers in the literature discuss the potential risks related to NP exposure, and more recently the concept that even sublethal doses of NPs may elicit a cell response has been proposed. In this review, we intend to present an overall view of cell mechanisms that may be perturbed by cell-NP interaction. Published data, in fact, emphasize that NPs should no longer be viewed only as simple carriers for biomedical applications, but that they can also play an active role in mediating biological effects.

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Polystyrene nanoparticles activate ion transport in human airway epithelial cells

Cited by 50 publications

References 42 publications

Analytical approaches to support current understanding of exposure, uptake and distributions of engineered nanoparticles by aquatic and terrestrial organisms

Analytical approaches to support current understanding of exposure, uptake and distributions of engineered nanoparticles by aquatic and terrestrial organisms

Amorphous silica nanoparticles aggregate human platelets: potential implications for vascular homeostasis

The effect of nanoparticle uptake on cellular behavior: disrupting or enabling functions?

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