Polysaccharides with complement fixing and macrophage stimulation activity from Opilia celtidifolia, isolation and partial characterisation

Togola, Adiaratou; Inngjerdingen, Marit; Diallo, Drissa; Barsett, Hilde; Rolstad, Bent; Michaelsen, Terje E.; Paulsen, Berit Smestad

doi:10.1016/j.jep.2007.10.017

Cited by 70 publications

(57 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Polysaccharide compounds in other plants (such as Solanum nigrum, Opilia celtidifolia, Potentilla anserina) have been reported to have immunomodulating activities [12][13][14]. In the present study, we confirmed these properties also in polysaccharide fraction C of Caltha palustris.…”

Section: Discussionsupporting

confidence: 86%

Experimental immunology Influence of polysaccharide fraction C isolated from Caltha palustris L. on T and B lymphocyte subsets in mice

Suszko¹,

Szczypka²,

Lis³

et al. 2012

cejoi

View full text Add to dashboard Cite

show abstract

Section: Discussionsupporting

confidence: 86%

Experimental immunology Influence of polysaccharide fraction C isolated from Caltha palustris L. on T and B lymphocyte subsets in mice

Suszko¹,

Szczypka²,

Lis³

et al. 2012

cejoi

View full text Add to dashboard Cite

show abstract

“…Since NO, IFN-c, and TNF-a play a critical in the antimicrobial and tumoricidal activities of macrophages, great attention has been paid to developing drugs that modulate NO and TNF-a production (Poderoso et al, 1999). Many polysaccharides can activate macrophages by inducing the production of cytokines (Belska et al, 2010;Lee et al, 2010;Togola et al, 2008). Our study showed that AMP promoted NO, TNF-a, and IFN-c secretion, indicating its stimulatory effect in the host defense against microbial infection on activated macrophages.…”

Section: Discussionmentioning

confidence: 60%

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Chen

Zheng

2014

Pharmaceutical Biology

View full text Add to dashboard Cite

Context: Atractylodes macrocephala Koidz is a traditional herb. Atractylodes macrocephalaon polysaccharides (AMP) have been found to enhance immunity and improve heart function. However, the mechanisms of the immunomodulatory effect have not been investigated. Objective: We examined whether AMP activated macrophages and explored the mechanisms of activation. Materials and methods: AMP was prepared and evaluated its immunomodulatory activity (25, 50, 100, and 200 lg/mL) by detecting the phagocytosis and the production of tumor necrosis factor-a (TNF-a), IFN-c, and nitric oxide (NO) in RAW264.7 macrophages. Furthermore, the role of nuclear factor-kB (NF-kB) pathway was examined in regulating TNF-a and NO production. Results: The phagocytosis of macrophages was enhanced by AMP in a dose-dependent manner and the maximal phagocytosis of macrophages occurred at concentrations of 100 and 200 lg/mL. NO, TNF-a, and IFN-c release was also found to be dose dependent by increasing concentrations of AMP and reached the peak at a concentration of 200 lg/mL. In addition, AMP induced inhibitor kappaB (IkB) degradation and the activation of NF-kB by p65 nuclear translocation, and then the activation of NF-kB in nucleus peaked at a concentration of 200 lg/ mL. Besides, NF-kB-specific inhibitor pyrrolidine dithiocarbamate (PDTC) decreased AMP-induced NO and TNF-a production. Discussion and conclusion: These data suggest that AMP may modulate macrophage activities by stimulating NF-kB or activating NF-kB-dependent mechanisms.

show abstract

“…The high molecular weight sub-fractions-I contained almost equal amounts of AG-I and AG-II, which may be important for the high complement fixing activity. The combination of AG-I and AG-II side chains have been reported to exhibit higher complement fixating activities than with only AG-II side chains alone [47,105]. Arabinan are present in a high amount in 100WSnFl-I-S2-I, and might also influence the activity.…”

mentioning

confidence: 99%

Structure–activity relationship of immunomodulating pectins from elderberries

Ahmed

Zou

et al. 2015

Carbohydrate Polymers

Self Cite

View full text Add to dashboard Cite

Polysaccharides with complement fixing and macrophage stimulation activity from Opilia celtidifolia, isolation and partial characterisation

Cited by 70 publications

References 34 publications

Experimental immunology Influence of polysaccharide fraction C isolated from Caltha palustris L. on T and B lymphocyte subsets in mice

Experimental immunology Influence of polysaccharide fraction C isolated from Caltha palustris L. on T and B lymphocyte subsets in mice

Macrophage activation by polysaccharides fromAtractylodes macrocephalaKoidz through the nuclear factor-κB pathway

Structure–activity relationship of immunomodulating pectins from elderberries

Contact Info

Product

Resources

About