Polysaccharides-Rich Extract ofGanoderma lucidum(M.A. Curtis:Fr.) P. Karst Accelerates Wound Healing in Streptozotocin-Induced Diabetic Rats

Abstract

Ganoderma lucidum (M.A. Curtis:Fr.) P. Karst is a popular medicinal mushroom. Scientific reports had shown that the wound healing effects of G. lucidum were partly attributed to its rich polysaccharides. However, little attention has been paid to its potential effects on wounds associated with diabetes mellitus. In this study, we evaluated the wound healing activity of the hot aqueous extract of G. lucidum in streptozotocin-induced diabetic rats. The extract of G. lucidum was standardised based on chemical contents (w/w) of total polysaccharides (25.1%), ganoderic acid A (0.45%), and adenosine (0.069%). Six groups of six rats were experimentally wounded in the posterior neck region. Intrasite gel was used as a positive control and aqueous cream as the placebo. Topical application with 10% (w/w) of mushroom extract-incorporated aqueous cream was more effective than that with Intrasite gel in terms of wound closure. The antioxidant activity in serum of rats treated with aqueous extract of G. lucidum was significantly higher; whereas the oxidative protein products and lipid damage were lower when compared to those of the controls. These findings strongly support the beneficial effects of standardised aqueous extract of G. lucidum in accelerating wound healing in streptozotocin-induced diabetic rats.

Designer fibers from 2D to 3D – Simultaneous and controlled engineering of morphology, shape and size

“…Despite the aforementioned properties of GL, ganoderma lucidum spores polysaccharide (GLSP) have been identified for antidiabetic and tissue repair benefits, especially for the treatment of chronic cutaneous wounds caused by diabetes mellitus and vascular indications (e.g. such as diabetic foot ulcers), which carry the risk of limb amputation [16,17]. In-vivo experiments of GLSP present no toxicity, implying good biocompatibility, while in practice, β-D-glucan (one form of polysaccharide isolated from GLSP) is highly sensitive to oxidative degradation, including the reactions of active oxygen substances, such as hydroxyl radicals [18].…”

Application ofAntrodia camphorataPromotes Rat’s Wound HealingIn Vivoand Facilitates Fibroblast Cell ProliferationIn Vitro

“…Wound healing mechanisms may be attributed to motivating the manufacture of antioxidants in wound location and provide encouraging background for tissue healing [ 3 , 24 ]. Antioxidants play an important part in the course of wound curing by improving the curative time and the manifestation of the healed tissue, while defensive tissues from oxidative injure [ 3 , 5 , 6 ]. The wound healing achievement of Antrodia camphorata may be due to the antioxidant present in the Antrodia camphorata and also elevated free radical scavenging action.…”

Ointment ofBrassica oleraceavar.capitataMatures the Extracellular Matrix in Skin Wounds of Wistar Rats

“…The use of medicinal plants stimulates the production of connective and epithelial tissues that are important to form the framework and promote wound closure [ 16 , 25 ]. The area and the wound closing speed are important for healing, as they serve as markers to observe the evolution of the wound [ 26 ]. The smaller area and the fastest speed of wound contraction in the groups treated with balm and B. oleracea ointment indicate the action of its components in cell proliferation and differentiation.…”

“…Curtis:Fr.) P. Karst, a popular medicinal mushroom, rich in flavonoids and phenols, a compound also present in B. oleracea [ 26 – 28 ]. This compound is responsible for therapeutic effects, such as antioxidant, anti-inflammatory, and antifungal effect, and also known to promote quick healing because of its antimicrobial properties that promote complete collagen synthesis [ 28 , 29 ].…”

“…These data can be demonstrated through the analysis of tissues of rats that received olive oil application [ 17 ] and male albino rats that received topical ointment of the J. grandiflorum extract [ 24 ]. Tissue repair resulted from the catabolic and anabolic reactions repeated by different cell types, and hence, the increased cellularity indicated that the repair process was successful in reestablishing homeostasis and scar formation [ 26 ].…”