Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens

Vunduk, Jovana; Wan‐Mohtar, Wan Abd Al Qadr Imad; Mohamad, Shaiful Azuar; Halim, Nur Hafizati Abd; Dzomir, Ahmad Zainuri Mohd; Žižak, Željko; Klaus, Anita

doi:10.1016/j.lwt.2019.05.119

Cited by 22 publications

(22 citation statements)

References 27 publications

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“…Suspensions of overnight cultures were adjusted to a final concentration of 10 8 colony forming units (CFU)/mL on a DEN-1 McFarland densitometer (Biosan, Latvia). The antiadhesion ability was estimated according to a modified method of Valeriano et al (2014) and Vunduk et al (2019). Antiadhesion assays were performed in flat bottomed 96-well polystyrene microtitre plates (Tissue Culture Testplate; SPL Life Sciences, Pocheon, Korea) using pig ileal mucin (1 mg mL − 1 and) as a matrix.…”

Section: Evaluation Of the Antiadhesion Ability In Mucinmentioning

confidence: 99%

“…The antiadhesion ability of OP powders (2% m/v) relative to food contaminant/pathogenic bacteria is reported in Table 3. However, it is essential to clarify that the inhibition of bacterial adhesion can be the result of various mechanisms such as cell surface modification, quorum sensing, or changes in the cell structure that are not directly connected with the antibacterial activity of the tested substance (Vunduk et al, 2019).…”

Section: Antiadhesion Ability In Mucinmentioning

confidence: 99%

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Prebiotic effects of olive pomace powders in the gut: In vitro evaluation of the inhibition of adhesion of pathogens, prebiotic and antioxidant effects

et al. 2021

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Olive pomace is a biowaste rich in polyphenols and insoluble dietary fibre with high potential to develop new value chains towards a sustainable and circular bioeconomy. Regarding gut health, olive pomace phenolics and insoluble dietary fibre (after possible fermentation) could act as antioxidants, antimicrobial and prebiotic agents. These potential beneficial effects on the gut were analysed for two powders from olive pomace: liquid-enriched powder (LOPP)-mostly source of phenolics-and pulp-enriched powder (POPP)-main source of insoluble dietary fibre. LOPP and POPP were subjected to an in vitro simulated gastrointestinal digestion followed by in vitro faecal fermentation. The undigested fraction retained in the colon was analysed regarding its potential antioxidant, antimicrobial and prebiotic effects. LOPP and POPP did not impact the gut microbiota diversity negatively, showing a similar ratio of Firmicutes/Bacteroidetes compared to a positive control (FOS). LOPP exhibit a positive (similar to FOS) effect on the Prevotella spp./Bacteroides spp. ratio. Both powders promoted more the production of short-chain fatty acids (mainly acetate > butyrate > propionate) than FOS and showed also significant total phenolic content and oxygen radical absorbance capacity during faecal fermentation until 48 h. Besides that, these powders showed mucin-adhesion inhibition ability against pathogens, principally POPP against Bacillus cereus (22.03 ± 2.45%) and Listeria monocytogenes (20.01 ± 1.93%). This study demonstrates that olive pomace powders have prebiotic effects on microbiota, including the stimulation of short-chain fatty acids production, potential antioxidant and antimicrobial activity which could improve the human gut health.

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Section: Evaluation Of the Antiadhesion Ability In Mucinmentioning

confidence: 99%

Section: Antiadhesion Ability In Mucinmentioning

confidence: 99%

Prebiotic effects of olive pomace powders in the gut: In vitro evaluation of the inhibition of adhesion of pathogens, prebiotic and antioxidant effects

et al. 2021

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“…Fermentation strategies have been unchanged for decades [1] . The conventional way to study mushrooms was through solid-state fermentation (SSF) until submerged liquid fermentation (SLF) was improved [2] .…”

Section: Introductionmentioning

confidence: 99%

Pellet diameter of <em>Ganoderma lucidum</em> in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan response

et al. 2020

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The pellet morphology and diameter range (DR) of Ganoderma lucidum were observed in a repeated-batch fermentation (RBF) for the trio total production of biomass, exopolysaccharide (EPS) and endopolysaccharide (ENS). Two factors were involved in RBF; broth replacement ratio (BRR: 60%, 75% and 90%) and broth replacement time point (BRTP: log, transition and stationary phase) in days. In RBF, 34.31 g/L of biomass favoured small-compact pellets with DR of 20.67 µm< d < 24.00 µm (75% BRR, day 11 of BRTP). EPS production of 4.34 g/L was prone to ovoid-starburst pellets with DR of 34.33 µm< d <35.67 µm (75% BRR, day 13 of BRTP). Meanwhile, the highest 2.43 g/L of ENS production favoured large-hollow pellets with DR of 34.00 µm< d < 38.67 µm (90% BRR, day 13 of BRTP). In addition, RBF successfully shortened the biomass-EPS–ENS fermentation period (31, 33 and 35 days) from batch to 5 days, in seven consecutive cycles of RBF. In a FTIR detection, β-glucan (BG) from EPS and ENS extracts were associated with β-glycosidic linkages (2925 cm −1 , 1635 cm −1 , 1077 cm −1 , 920 cm −1 and 800 cm −1 wavelengths) with similar 1 H NMR spectral behaviour (4.58, 3.87 and 3.81 ppm). Meanwhile, 4 mg/L of BG gave negative cytotoxic effects on normal gingival cell line (hGF) but induced antiproliferation (IC 50 = 0.23 mg/mL) against cancerous oral Asian cellosaurus cell line (ORL-48). Together, this study proved that G. lucidum mycelial pellets could withstand seven cycles of long fermentation condition and possessed anti-oral cancer beta-glucan, which suits large-scale natural drug fermentation.

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“…EPS-ENS originates from edible mushroom species and has potential to be used as high-value product in the form of dietary fish feed supplement [ 29 ]. Recently, use of EPS-ENS (or beta-glucan) extracts has increased in interest for various applications, such as for preventing biofilms [ 30 ] and for fish-feed [ 10 ]. Beta-glucans derived from mushrooms and other sources, have also been reported as potential new therapeutic to help fight Coronavirus disease (COVID-19) in the form of potential immunotherapies [ 31 ]; to alleviate use of frontline antibiotics for combatting resilient bacteria [ 32 ]; as an alternative to the use of silver nanoparticles [ 33 ]; or for other potential applications such as cosmetics [ 34 ], immune priming or boosting [ 12 ], and prevention of cell death [ 2 ].…”

Section: Discussionmentioning

confidence: 99%

Use of Zebrafish Embryo Assay to Evaluate Toxicity and Safety of Bioreactor-Grown Exopolysaccharides and Endopolysaccharides from European Ganoderma applanatum Mycelium for Future Aquaculture Applications

Wan‐Mohtar

Ilham

Jamaludin

et al. 2021

IJMS

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Natural mycelial exopolysaccharide (EPS) and endopolysaccharide (ENS) extracted from bioreactor-cultivated European Ganoderma applanatum mushrooms are of potential high commercial value for both food and adjacent biopharmaceutical industries. In order to evaluate their potential toxicity for aquaculture application, both EPS (0.01–10 mg/mL) and ENS (0.01–10 mg/mL) extracts were tested for Zebrafish Embryo Toxicity (ZFET); early development effects on Zebrafish Embryos (ZE) were also analyzed between 24 and 120 h post-fertilization (HPF). Both EPS and ENS are considered non-toxic with LC50 of 1.41 mg/mL and 0.87 mg/mL respectively. Both EPS and ENS did not delay hatching and teratogenic defect towards ZE with <1.0 mg/mL, respectively. No significant changes in the ZE heart rate were detected following treatment with the two compounds tested (EPS: 0.01–10 mg/mL: 176.44 ± 0.77 beats/min and ENS: 0.01–10 mg/mL: 148.44 ± 17.75 beats/min) compared to normal ZE (120–180 beats/min). These initial findings support future pre-clinical trials in adult fish models with view to safely using EPS and ENS as potential feed supplements for supplements for development of the aquaculture industry.

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Polysaccharides of Pleurotus flabellatus strain Mynuk produced by submerged fermentation as a promising novel tool against adhesion and biofilm formation of foodborne pathogens

Cited by 22 publications

References 27 publications

Prebiotic effects of olive pomace powders in the gut: In vitro evaluation of the inhibition of adhesion of pathogens, prebiotic and antioxidant effects

Prebiotic effects of olive pomace powders in the gut: In vitro evaluation of the inhibition of adhesion of pathogens, prebiotic and antioxidant effects

Pellet diameter of <em>Ganoderma lucidum</em> in a repeated-batch fermentation for the trio total production of biomass-exopolysaccharide-endopolysaccharide and its anti-oral cancer beta-glucan response

Use of Zebrafish Embryo Assay to Evaluate Toxicity and Safety of Bioreactor-Grown Exopolysaccharides and Endopolysaccharides from European Ganoderma applanatum Mycelium for Future Aquaculture Applications

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