Polysaccharides involved in slime-mold development I. Water-soluble glucose polymer(s)

White, Gordon J.; Sussman, Maurice

doi:10.1016/0006-3002(63)91355-6

Cited by 42 publications

(12 citation statements)

References 12 publications

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“…The glucose polymer found in the alkali-soluble fraction of the cyst walls of H. glebae is suspected to be a similar P-glucan. In a cellular slime mold, Dictyosteliurn discoideum cellulose and cell wall-bound glycogen are present only in insignificant quantities in the myxamoebae ( 14). The accumulation of cellulose and glycogen represents the major changes that occur in the gross chemical composition of the cell from the vegetative to the microcyst stage (1 1).…”

mentioning

confidence: 99%

The Cyst Wall Composition of Hartmannella glebae

Upadhyay

Crow

Cox

1984

Experimental Biology and Medicine

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In this study cyst walls of Hartmannella glebae were isolated and quantitatively analyzed. They were fractionated into alkali-insoluble and alkali-soluble fractions. The alkaliinsoluble fraction appeared to be cellulose which represented 4.2% of the total weight of the wall. The major component of the alkali-soluble fraction consisted of proteins (64.7%). It also contained a glucose polymer (probably a precursor of cellulose) and lipids. The amino acid composition of the wall was also determined. 424

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confidence: 99%

The Cyst Wall Composition of Hartmannella glebae

Upadhyay

Crow

Cox

1984

Experimental Biology and Medicine

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“…Glycogen is the only polysaccharide that has been detected in amoebae during growth and early development (White & Sussman, 1963a; but, during fruiting body construction, two new polysaccharides appear. Cellulose is synthesized in spore and stalk cells (Ward & Wright, 1965) and an antigenic mucopolysaccharide containing galactose, galactosamine and galacturonic acid (White & Sussman, 1963b) in the spores.…”

Section: Developmentally Regulated Enzymesmentioning

confidence: 99%

Protein synthesis during development and differentiation in the cellular slime mould Dictyostelium discoideum

Watts¹

1984

Biochemical Journal

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“…Samples of homogenized cells were fractionated into three portions, (1) alkali-insoluble, (2) alkali-soluble and ethanol-insoluble, and (3) alkali-and ethanolsoluble, according to the methods of Ward and Wright (41) and White and Sussman (43,44), with minor modifications . Each fraction was resuspended in water and its total carbohydrate content was estimated by the anthrone method, with glucose as a standard (2) .…”

Section: Carbohydrate Fractionation and Analysismentioning

confidence: 99%

“…The patterns of the changes in the individual fractions also resemble those seen during the development of D . discoideum on a surface (43,44) . However, the absolute levels of Fractions 2 and 3 were much lower in experiments with D .…”

Section: Biochemical Aspects Of Encystment In Smmentioning

confidence: 99%

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BIOCHEMICAL CHANGES DURING GROWTH AND ENCYSTMENT OF THE CELLULAR SLIME MOLD POLYSPHONDYLIUM PALLIDUM

Githens

Karnovsky

1973

The Journal of Cell Biology

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The growth of the cellular slime mold, Polysphondylium pallidum, was studied on a semidefined medium in shaken suspension . When the medium contained large quantities of particulate material, growth was more rapid and the cellular size and protein content were smaller than when growth occurred on a medium containing less particulate material . The cellular levels of DNA, RNA, and protein ; of lysosomal enzymes (acid phosphatase, acid proteinase) ; and of peroxisomal enzymes (catalase) were assayed during growth and the subsequent stationary phase that led eventually to encystment . Only DNA remained at a constant cellular level . Encystment of exponentially growing cells could also be initiated by washing them and introducing them into a soluble peptone medium . The rate of encystment was proportional to the osmolarity of this medium . The encystment process was followed with respect to the cellular levels of DNA, RNA, protein, carbohydrates, acid phosphatase, acid (3-N-Acglucosaminidase, and catalase . The most dramatic change occurred in the cellular cellulose content, which increased by at least an order of magnitude by the time encystment was morphologically complete . It was concluded that the encystment of this slime mold in suspension exhibits a number of biochemical similarities to the development of this and other cellular slime molds on a surface .

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Polysaccharides involved in slime-mold development I. Water-soluble glucose polymer(s)

Cited by 42 publications

References 12 publications

The Cyst Wall Composition of Hartmannella glebae

The Cyst Wall Composition of Hartmannella glebae

Protein synthesis during development and differentiation in the cellular slime mould Dictyostelium discoideum

BIOCHEMICAL CHANGES DURING GROWTH AND ENCYSTMENT OF THE CELLULAR SLIME MOLD POLYSPHONDYLIUM PALLIDUM

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