Polysaccharides from the seeds of <i>Gleditsia sinensis</i> Lam. attenuate DSS-induced colitis in mice <i>via</i> improving gut barrier homeostasis and alleviating gut microbiota dybiosis

Qin, Ningbo; Liu, Hongxu; Cao, Yu; Wang, Zhen; Ren, Xiaomeng; Xia, Xiaodong

doi:10.1039/d2fo02722d

Cited by 11 publications

(4 citation statements)

References 58 publications

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“…The sequences of the gene-specific primers used in the experiment are shown in Table 1. 49,50 Statistical Analysis. In this study, all data are shown as mean ± standard deviation (SD) from three independent measurements (n = 3).…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

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Mucoadhesive Hydrogel with Anti-gastric Acid and Sustained-Release Functions for Amelioration of DSS-Induced Ulcerative Colitis

Huang

Gong

Hou

et al. 2023

J. Agric. Food Chem.

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Mucoadhesive hydrogels with multifunctional properties such as gastric acid resistance and sustained drug release in the intestinal tract are highly desirable for the oral treatment of inflammatory bowel diseases (IBDs). Polyphenols are proven to have great efficacies compared with the first-line drugs for IBD treatments. We recently reported that gallic acid (GA) was capable of forming a hydrogel. However, this hydrogel is prone to easy degradation and poor adhesion in vivo. To tackle this problem, the current study introduced sodium alginate (SA) to form a gallic acid/sodium alginate hybrid hydrogel (GAS). As expected, the GAS hydrogel showed excellent antiacid, mucoadhesive, and sustained degradation properties in the intestinal tract. In vitro studies demonstrated that the GAS hydrogel significantly alleviated ulcerative colitis (UC) in mice. The colonic length of the GAS group (7.75 ± 0.38 cm) was significantly longer than that of the UC group (6.12 ± 0.25 cm). The disease activity index (DAI) value of the UC group was (5.5 ± 0.57), which was markedly higher than that of the GAS group (2.5 ± 0.65). The GAS hydrogel also could inhibit the expression of inflammatory cytokines, regulating macrophage polarization and improving the intestinal mucosal barrier functions. All these results indicated that the GAS hydrogel was an ideal candidate for oral treatment of UC.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

“…The 2 –ΔΔCt method was used to calculate the relative expression level of all target genes, and glyceraldehyde-3-phosphatase dehydrogenase (GAPDH) was used as a normalized control. The sequences of the gene-specific primers used in the experiment are shown in Table . , …”

Section: Methodsmentioning

confidence: 99%

Mucoadhesive Hydrogel with Anti-gastric Acid and Sustained-Release Functions for Amelioration of DSS-Induced Ulcerative Colitis

Huang

Gong

Hou

et al. 2023

J. Agric. Food Chem.

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“…Then, the sections were stained using hematoxylin and eosin, and the resulting stain observations were photographed and analyzed with an inverted microscope. The detection of the tight junction protein (ZO-1) in colonic tissues was conducted through immunohistochemistry [26]. The fixed colon tissue was sectioned and dewaxed, and then the antigen repair and BSA closure were performed.…”

Section: Hematoxylin and Eosin (Hande) Staining And Immunofluorescenc...mentioning

confidence: 99%

Wheat Bran Polyphenols Ameliorate DSS-Induced Ulcerative Colitis in Mice by Suppressing MAPK/NF-κB Inflammasome Pathways and Regulating Intestinal Microbiota

Wen,

Peng,

Zhang

et al. 2024

Foods

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Wheat bran (WB) is the primary by-product of wheat processing and contains a high concentration of bioactive substances such as polyphenols. This study analyzed the qualitative and quantitative components of polyphenols in wheat bran and their effects on ulcerative colitis (UC) using the dextran sulfate sodium (DSS)-induced colitis model in mice. The potential mechanism of wheat bran polyphenols (WBP) was also examined. Our findings indicate that the main polyphenol constituents of WBP were phenolic acids, including vanillic acid, ferulic acid, caffeic acid, gallic acid, and protocatechuic acid. Furthermore, WBP exerted remarkable protective effects against experimental colitis. This was achieved by reducing the severity of colitis and improving colon morphology. Additionally, WBP suppressed colonic inflammation via upregulation of the anti-inflammatory cytokine IL-10 and downregulation of pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) in colon tissues. Mechanistically, WBP ameliorated DSS-induced colitis in mice by inhibiting activation of the MAPK/NF-κB pathway. In addition, microbiome analysis results suggested that WBP modulated the alteration of gut microbiota caused by DSS, with an enhancement in the ratio of Firmicutes/Bacteroidetes and adjustments in the number of Helicobacter, Escherichia-Shigella, Akkermansia, Lactobacillus, Lachnospiraceae_NK4A136_group at the genus level. To conclude, the findings showed that WBP has excellent prospects in reducing colonic inflammation in UC mice.

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“…The intestine is the largest barrier between organisms and environment, protecting the body from harmful external substances. The intestinal barrier mainly consists of four parts, namely physical, chemical, immunological, and microbiological barriers, among which the physical barrier is an intact intestinal epithelium structure consisting of intestinal mucosal epithelial cells through tight junction proteins, such as occludin, claudins, zonula occludens (ZOs), and tricellulin [8,9]. Food, especially high-fat and high-fructose food, can cause great damage to the intestinal barrier [10].…”

Section: Introductionmentioning

confidence: 99%

Artemether Attenuates Gut Barrier Dysfunction and Intestinal Flora Imbalance in High-Fat and High-Fructose Diet-Fed Mice

Ren,

Xu,

et al. 2023

Nutrients

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Intestinal inflammation is a key determinant of intestinal and systemic health, and when our intestines are damaged, there is disruption of the intestinal barrier, which in turn induces a systemic inflammatory response. However, the etiology and pathogenesis of inflammatory diseases of the intestine are still not fully understood. Artemether (ART), one of the artemisinin derivatives, has been widely used to treat malaria. Nevertheless, the effect of ART on intestinal inflammation remains unclear. The present study intended to elucidate the potential mechanism of ART in diet-induced intestinal injury. A high-fat and high-fructose (HFHF) diet-induced mouse model of intestinal injury was constructed, and the mice were treated with ART to examine their role in intestinal injury. RT-qPCR, Western blotting, immunohistochemical staining, and 16S rRNA gene sequencing were used to investigate the anti-intestinal inflammation effect and mechanism of ART. The results indicated that ART intervention may significantly ameliorate the intestinal flora imbalance caused by the HFHF diet and alleviate intestinal barrier function disorders and inflammatory responses by raising the expression of tight junction proteins ZO-1 and occludin and decreasing the expression of pro-inflammatory factors TNF-α and IL-1β. Moreover, ART intervention restrained HFHF-induced activation of the TLR4/NF-κB p65 pathway in colon tissue, which may be concerned with the potential protective effect of ART on intestinal inflammation. ART might provide new insights into further explaining the mechanism of action of other metabolic diseases caused by intestinal disorders.

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Polysaccharides from the seeds of Gleditsia sinensis Lam. attenuate DSS-induced colitis in mice via improving gut barrier homeostasis and alleviating gut microbiota dybiosis

Abstract: The polysaccharides from seeds of Gleditsia sinensis Lam. alleviate DSS-induced colitis in mice.

Cited by 11 publications

References 58 publications

Mucoadhesive Hydrogel with Anti-gastric Acid and Sustained-Release Functions for Amelioration of DSS-Induced Ulcerative Colitis

Mucoadhesive Hydrogel with Anti-gastric Acid and Sustained-Release Functions for Amelioration of DSS-Induced Ulcerative Colitis

Wheat Bran Polyphenols Ameliorate DSS-Induced Ulcerative Colitis in Mice by Suppressing MAPK/NF-κB Inflammasome Pathways and Regulating Intestinal Microbiota

Artemether Attenuates Gut Barrier Dysfunction and Intestinal Flora Imbalance in High-Fat and High-Fructose Diet-Fed Mice

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