Polysaccharides from Opuntia milpa alta alleviate alloxan-induced INS-1 cells apoptosis via reducing oxidative stress and upregulating Nrf2 expression

Li, Weiling; Lin, Kuan; Xiong, Qi; Li, Chaoying; Ru, Qin

doi:10.1016/j.nutres.2020.02.004

Cited by 21 publications

(12 citation statements)

References 37 publications

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“…Cell cytotoxicity and protection of TPE and TPB was measured by CCK-8 assay kit following the manufacturer’s specifications as a reference protocol. SOD, GSH-P x, MDA and ROS were measured using the kits following the specifications [ 42 ].…”

Section: Methodsmentioning

confidence: 99%

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury

et al. 2022

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Current therapies for ischemic stroke are insufficient due to the lack of specific drugs. This study aimed to investigate the protective activity of polyphenol extracts from Terminalia chebula against cerebral ischemia-reperfusion induced damage. Polyphenols of ethyl acetate and n-butanol fractions were extracted from T. chebula. BV2 microglial cells exposed to oxygen-glucose deprivation/reoxygenation and mice subjected to middle cerebral artery occlusion/reperfusion were treated by TPE and TPB. Cell viability, cell morphology, apoptosis, mitochondrial membrane potential, enzyme activity and signaling pathway related to oxidative stress were observed. We found that TPE and TPB showed strong antioxidant activity in vitro. The protective effects of TPE and TPB on cerebral ischemia-reperfusion injury were demonstrated by enhanced antioxidant enzyme activities, elevated level of the nucleus transportation of nuclear factor erythroid 2-related factor 2 and expressions of antioxidant proteins, with a simultaneous reduction in cell apoptosis and reactive oxygen species level. In conclusion, TPE and TPB exert neuroprotective effects by stimulating the Nrf2 signaling pathway, thereby inhibiting apoptosis.

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Section: Methodsmentioning

confidence: 99%

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury

et al. 2022

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“…MAP restored cell viability and SOD and GSH activity, while considerably decreased the release of ROS, LDH, MDA and nitric oxide (NO) levels. Possibly, MAP may attenuate the apoptosis induced by Allox by increasing the expression of Bcl-2, decreasing the expression of Bax and the activities of caspase-3 and caspase-9 [79].…”

Section: Hepatoprotective Effectmentioning

confidence: 99%

Opuntia genus in Human Health: A Comprehensive Summary on Its Pharmacological, Therapeutic and Preventive Properties. Part 1

et al. 2022

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Opuntia spp. is a diverse and widely distributed genus in Africa, Asia, Australia, and America. Mexico has the largest number of wild species; mainly O. streptacantha, O. hyptiacantha, O. albicarpa, O. megacantha and O. ficus-indica. The latter being the most cultivated and domesticated species. Throughout history, plants and their phytochemicals have played an important role in health care and Opuntia spp. has shown a high nutritional, medicinal, pharmaceutical, and economic impacts. Its main bioactive compounds include pigments (carotenoids, betalains and betacyanins), vitamins, flavonoids (isorhamnetin, kaempferol, quercetin) and phenolic compounds. Together, they favor the different plant parts and are considered phytochemically important and associated with control, progression and prevention of some chronic and infectious diseases. This first review (Part 1), compiles information from published research (in vitro, in vivo, and clinical studies) on its preventive effects against atherosclerotic cardiovascular diseases, diabetes and obesity, hepatoprotection, effects on human infertility and chemopreventive and/or antigenotoxic capacity. The aim is to provide scientific evidences of its beneficial properties and to encourage health professionals and researchers to expand studies on the pharmacological and therapeutic effects of Opuntia spp.

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“…6 Excessive, uncontrolled oxidative stress contributes to pancreatic β-cell dysfunction, therefore, studies on the precise role of antioxidant agents in mediating β-cell insulin secretion and survival are continuing，which will provide a view of potential treatments for DM. 7 PDX-1, or pancreatic duodenal homologous box factor-1, is a transcription factor that plays a key role in the growth and function of pancreatic β-cells. 8 Oxidative stress can not only cause the decrease of PDX-1 protein expression, but also lead to abnormal nuclear plasmic translocation of PDX-1, which may be the main reason for the decrease of PDX-1 binding to insulin mRNA.…”

Section: Introductionmentioning

confidence: 99%

“…It is widely accepted that increased oxidative phosphorylation leads to elevated ROS production in β‐cells when blood glucose is chronically elevated in T2DM 6 . Excessive, uncontrolled oxidative stress contributes to pancreatic β‐cell dysfunction, therefore, studies on the precise role of antioxidant agents in mediating β‐cell insulin secretion and survival are continuing，which will provide a view of potential treatments for DM 7 …”

Section: Introductionmentioning

confidence: 99%

Swietenine and swietenolide from Swietenia macrophylla king improve insulin secretion and attenuate apoptosis in H₂O₂ induced INS‐1 cells

Duan

Zhang

et al. 2022

Environmental Toxicology

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Oxidative stress is an important factor that causes pancreatic β‐cell dysfunction leading to the development and aggravation of diabetes. Swietenine (Stn) and swietenolide (Std) were isolated from the fruits of Swietenia macrophylla King and had the potential effects on treatment and prevention of diabetes. The aim of this study is to investigate the effects of Stn and Std on insulin secretion and apoptosis in H2O2 induced insulinoma cell line (INS‐1) cells. In the present study, INS‐1 cells were treated with 300 μM H2O2 for 4 h to establish the oxidative damage model. Cell apoptosis, insulin secretion, reactive oxygen species (ROS), superoxide dismutase (SOD), malondialdehyde (MDA), and glutathione (GSH) levels, and Caspase‐3 enzyme activity were measured via corresponding methods. Finally, pancreatic duodenal home box factor‐1 (PDX‐1), B cell lymphoma‐2 (Bcl‐2), and Bax protein expression were detected by western blot. Experimental results showed that Stn and Std could significantly improve the INS‐1 cell viability, increase the secretion of insulin and reduce the ROS level in H2O2 induced INS‐1 cells. Furthermore, the SOD and GSH levels increased, and the MDA levels decreased compared with the model group after Stn and Std treatment. In addition, after treated with Stn and Std, cell apoptosis was improved, and the activity of Caspase 3 was also significantly inhibited. Meanwhile, Western blot results showed that Stn and Std could up‐regulate the expression of PDX‐1 protein, and affect the cell apoptosis pathway by up‐regulating the expression of Bcl‐2 protein and down‐regulating the expression of Bax protein. In conclusion, Stn and Std can signifcantly improve the insulin secretion function, protect oxidative stress injury, and reduce apoptosis in H2O2 induced INS‐1 cells, which provides a research basis for Stn and Std to be new drug candidates for the treatment and prevention of diabetes.

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Polysaccharides from Opuntia milpa alta alleviate alloxan-induced INS-1 cells apoptosis via reducing oxidative stress and upregulating Nrf2 expression

Cited by 21 publications

References 37 publications

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury

Opuntia genus in Human Health: A Comprehensive Summary on Its Pharmacological, Therapeutic and Preventive Properties. Part 1

Swietenine and swietenolide from Swietenia macrophylla king improve insulin secretion and attenuate apoptosis in H₂O₂ induced INS‐1 cells

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Polysaccharides from Opuntia milpa alta alleviate alloxan-induced INS-1 cells apoptosis via reducing oxidative stress and upregulating Nrf2 expression

Cited by 21 publications

References 37 publications

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury

Neuroprotective Effect of Polyphenol Extracts from Terminalia chebula Retz. against Cerebral Ischemia-Reperfusion Injury

Opuntia genus in Human Health: A Comprehensive Summary on Its Pharmacological, Therapeutic and Preventive Properties. Part 1

Swietenine and swietenolide from Swietenia macrophylla king improve insulin secretion and attenuate apoptosis in H2O2 induced INS‐1 cells

Contact Info

Product

Resources

About

Swietenine and swietenolide from Swietenia macrophylla king improve insulin secretion and attenuate apoptosis in H₂O₂ induced INS‐1 cells