Polysaccharides fromNostoc communeVaucher activate macrophagesviaNF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growthin vivo

Guo, Min; Li, Zhuoyu; Huang, Yexin; Shi, Mengai

doi:10.1039/c9fo00595a

Cited by 22 publications

(11 citation statements)

References 22 publications

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“…Comparing our findings with previous research, the total polysaccharide yield with respect to biomass in this study was higher than that of most other water and organic extracts, such as Nannochloropsis oculata at 19% w / w [ 6 ]; Ulva lactuca at 26.01–28.29% w / w [ 5 ]; Parachlorella spp. at 73.8% w / w [ 26 ]; Chondrus verrucosus at 46.2–65.9% w / w [ 27 ]; and Nostoc commune at 82.2–84.6% w / w [ 28 ]. The results of this study suggest the feasibility of using C. microphysa as a cash crop to produce polysaccharides.…”

Section: Resultsmentioning

confidence: 99%

Extraction Procedure, Characteristics, and Feasibility of Caulerpa microphysa (Chlorophyta) Polysaccharide Extract as a Cosmetic Ingredient

2021

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The green alga Caulerpa microphysa, which is native to Taiwan, has a relatively high economic value and a well-developed culture technique, and is used mainly as a foodstuff. Its extract has been shown to exhibit antitumor properties, but the polysaccharide content of the extract and its anti-inflammatory and wound-healing effects and moisture-absorption and -retention capacity remain unknown. Hence, the objective of this study was to evaluate the potential of the polysaccharides in C. microphysa extract (CME) for use in cosmetics. The overall polysaccharide yield from the CME was 73.93% w/w, with four molecular weight fractions. The polysaccharides comprised 59.36 mol% mannose, 27.16 mol% glucose, and 13.48 mol% galactose. In addition, the CME exhibited strong antiallergic, wound-healing, transdermal-delivery, and moisture-absorption and -retention effects. In conclusion, the results suggested that CME potentially has anti-inflammatory and wound-healing effects and a good moisture capacity, which can be used in cosmetic applications.

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Section: Resultsmentioning

confidence: 99%

Extraction Procedure, Characteristics, and Feasibility of Caulerpa microphysa (Chlorophyta) Polysaccharide Extract as a Cosmetic Ingredient

2021

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“…The results showed that the polysaccharides from P. nobilis significantly inhibited the viability of colorectal cancer cells and suppressed cell clone level in a dose-dependent manner. This may be related to the fact that the polysaccharides of P. nobilis blocked the cell cycle at S-G2/M stage, thereby suppressing cell proliferation and division, and inhibiting cell apoptosis [8].…”

Section: Discussionmentioning

confidence: 99%

Brassica juncea polysaccharides induce apoptosis of colorectal cancer cells via mitochondrial- and caspasedependent apoptosis pathways

Li¹,

Yu²,

Chen³

2021

Trop. J. Pharm Res

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Purpose: To determine the effect of polysaccharides from Gleditsia on apoptosis of colorectal cancer cells, and the mechanism involved.Methods: Polysaccharides were extracted from Lycium barbarum, and their concentration was more than 85 %. Then, DLD-1 cells were cultured in medium with the polysaccharides at concentrations of 75 and 150 μg/mL. Cell proliferation was determined with MTT (3-(4,5)-dimethylthiahiazo (-z-y1)-3,5- diphenytetrazoliumromide) assay and colony formation assay, while apoptosis was determined with flow cytometry. Changes in MMP were measured flow cytometrically. The protein levels of PARP, Bcl-2, Bax, and caspases 3 and 9 were determined with Western blot assay.Results: Cell viability decreased time-dependently. Compared with control without polysaccharide exposure, cell viability, colony forming cells, % apoptosis, red: green fluorescence ratio, and bcl-2 expression were significantly and concentration-dependently decreased, while the expression levels of PARP, Bax, caspase-3 and caspase-8 were significantly increased (p < 0.05).Conclusion: These results indicate that the polysaccharides suppressed apoptosis of colorectal cancer cells by inhibiting the mitochondrial and caspase-dependent apoptosis pathways. Gleditsia polysaccharide may be used as an adjuvant therapy for colorectal cancer.

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“…Nuclear translation of p65 might promote the expression and secretion of these cytokines. Furthermore, Akt has been reported to participate in cell defense and immune regulation as an upstream regulator of NF-κB [39,40]. Activation of Akt results to inflammation by stimulating IKK phosphorylation in the NF-κB pathway [41].…”

Section: Discussionmentioning

confidence: 99%

Deacetyl Ganoderic Acid F Inhibits LPS-Induced Neural Inflammation via NF-κB Pathway Both In Vitro and In Vivo

et al. 2019

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Microglia mediated neuronal inflammation has been widely reported to be responsible for neurodegenerative disease. Deacetyl ganoderic acid F (DeGA F) is a triterpenoid isolated from Ganoderma lucidum, which is a famous edible and medicinal mushroom used for treatment of dizziness and insomnia in traditional medicine for a long time. In this study the inhibitory effects and mechanisms of DeGA F against lipopolysaccharide (LPS)-induced inflammation both in vitro and in vivo were investigated. On murine microglial cell line BV-2 cells, DeGA F treatment inhibited LPS-triggered NO production and iNOS expression and affected the secretion and mRNA levels of relative inflammatory cytokines. DeGA F inhibited LPS-induced activation of the NF-κB pathway, as evidenced by decreased phosphorylation of IKK and IκB and the nuclear translocation of P65. In vivo, DeGA F treatment effectively inhibited NO production in zebrafish embryos. Moreover, DeGA F suppressed the serum levels of pro-inflammatory cytokines, including TNF-α and IL-6 in LPS-stimulated mice model. DeGA F reduced inflammatory response by suppressing microglia and astrocytes activation and also suppressed LPS-induced NF-κB activation in mice brains. Taken together, DeGA F exhibited remarkable anti-inflammatory effects and promising therapeutic potential for neural inflammation associated diseases.

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Polysaccharides fromNostoc communeVaucher activate macrophagesviaNF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growthin vivo

Abstract: NVPS activate macrophages via NF-κB and AKT/JNK1/2 pathways to suppress colorectal cancer growth in vivo.

Cited by 22 publications

References 22 publications

Extraction Procedure, Characteristics, and Feasibility of Caulerpa microphysa (Chlorophyta) Polysaccharide Extract as a Cosmetic Ingredient

Extraction Procedure, Characteristics, and Feasibility of Caulerpa microphysa (Chlorophyta) Polysaccharide Extract as a Cosmetic Ingredient

Brassica juncea polysaccharides induce apoptosis of colorectal cancer cells via mitochondrial- and caspasedependent apoptosis pathways

Deacetyl Ganoderic Acid F Inhibits LPS-Induced Neural Inflammation via NF-κB Pathway Both In Vitro and In Vivo

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