Polysaccharides from <i>Dioscorea batatas</i> Induce Tumor Necrosis Factor-α Secretion via Toll-like Receptor 4-Mediated Protein Kinase Signaling Pathways

Liu, Ju-Yun; Fu, Yang; Lu, Chun‐Ping; Yang, Yu‐Liang; Wen, Chi-Luan; Hua, Kuo-Feng; Wu, Shih‐Hsiung

doi:10.1021/jf8018722

Cited by 38 publications

(24 citation statements)

References 29 publications

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“…5 extract, designated as DsII-TN5, effectively enhanced the DC-mediated activation of T-cell proliferation and suppressed the growth of melanoma tumor cells in vivo (Chang et al, 2013). Mechanistically, other studies showed that immunological activities of specific polysaccharides and/or glycoproteins in Dioscorea tubers were in part due to their TLR4-signaling pathway-mediated immunomodulatory and cytokine-regulation activities (Fu et al, 2006; Liu et al, 2008). Moreover, our previous study further showed that Dioscorea phytoextracts can enhance the proliferation of murine splenocytes ex vivo and promote regeneration of specific myeloid-derived progenitor cells in chemotherapy-damaged bone marrow tissues (Su et al, 2011).…”

Section: Regulation Of Immune Cells In the Tumor Microenvironment By mentioning

confidence: 99%

Phytochemicals Approach for Developing Cancer Immunotherapeutics

2017

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Phytochemicals or their derived compounds are being increasingly recognized as potentially potent complementary treatments for cancer. Among them, some phytochemicals are being actively evaluated for use as adjuvants in anticancer therapies. For instance, shikonin and hypericin were found to induce immunogenic cell death of specific cancer cells, and this effect was able to further activate the recognition activity of tumor cells by the host immune system. On the other hand, some derivatives of phytochemicals, such as dihydrobenzofuran lignan (Q2-3) have been found to induce the secretion of an endogenous anticancer factor, namely IL-25, from non-malignant cells. These findings suggest that phytochemicals or their derivatives confer a spectrum of different pharmacological activities, which contrasts with the current cytotoxic anticancer drugs commonly used in clinics. In this review, we have collected together pertinent information from recent studies about the biochemical and cellular mechanisms through which specific phytochemicals regulate target immune systems in defined tumor microenvironments. We have further highlighted the potential application of these immunotherapeutic modifiers in cell-based cancer vaccine systems. This knowledge provides useful technological support and know how for future applications of phytochemicals in cancer immunotherapy.

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Section: Regulation Of Immune Cells In the Tumor Microenvironment By mentioning

confidence: 99%

Phytochemicals Approach for Developing Cancer Immunotherapeutics

2017

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“…A specific polysaccharide fraction from Reishi stimulated immune cell activation including dendritic cell maturation and cytokine expression and displayed potent adjuvant activity in mice [153, 154]. Polysaccharides from Dioscorea batatas were found to induce TNF- α secretion via Toll-like receptor 4-mediated protein kinase signaling pathways [155]. A number of phytochemicals have also been demonstrated to effectively enhance the anti-tumor potency of gene-based cancer vaccines.…”

Section: Other Anticancer Applications Of Specific Herbal Medicinesmentioning

confidence: 99%

Therapeutic Applications of Herbal Medicines for Cancer Patients

Yin

Wei

Jian

et al. 2013

Evidence-Based Complementary and Alternative Medicine

268

170

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Medicinal herbs and their derivative phytocompounds are being increasingly recognized as useful complementary treatments for cancer. A large volume of clinical studies have reported the beneficial effects of herbal medicines on the survival, immune modulation, and quality of life (QOL) of cancer patients, when these herbal medicines are used in combination with conventional therapeutics. Here, we briefly review some examples of clinical studies that investigated the use of herbal medicines for various cancers and the development of randomized controlled trials (RCTs) in this emerging research area. In addition, we also report recent studies on the biochemical and cellular mechanisms of herbal medicines in specific tumor microenvironments and the potential application of specific phytochemicals in cell-based cancer vaccine systems. This review should provide useful technological support for evidence-based application of herbal medicines in cancer therapy.

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“…For example, polysaccharides ASP and G1-4A , which were isolated from Acanthopanax senticosus and Tinospora cordifolia , respectively, activated macrophages and B cells by interacting with TLR4 [54,55]. TLR4 was also found to be one of the cellular receptors mediating TNF secretion induced by ZPF1 , polysaccharide isolated from Dioscorea batatas [56]. Likewise, polysaccharide CWSP from Chlorella pyrenoidosa induced cytokine secretion in macrophages via TLR4-mediated signaling pathways [57].…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory activity of polysaccharides isolated from Clerodendrum splendens: Beneficial effects in experimental autoimmune encephalomyelitis

Kouakou

Schepetkin

Jun

et al. 2013

BMC Complement Altern Med

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BackgroundExtracts of leaves from Clerodendrum have been used for centuries to treat a variety of medicinal problems in tropical Africa. However, little is known about the high-molecular weight active components conferring therapeutic properties to these extracts.MethodsPolysaccharides from the leaves of Clerodendrum splendens were extracted and fractionated by ion exchange and size-exclusion chromatography. Molecular weight determination, sugar analysis, degree of methyl esterification, and other chemical characterization of the fractions were performed. Immunomodulatory activity of the fractions was evaluated by determining their ability to induce monocyte/macrophage nitric oxide (NO), cytokine production, and mitogen-activated protein kinase (MAPK) phosphorylation. Experimental autoimmune encephalomyelitis (EAE) was induced in C57BL/6 mice, and severity of EAE was monitored in mice treated with intraperitoneal (i.p.) injections of the most active polysaccharide fraction. Lymph nodes (LN) and spleen were harvested, and levels of cytokines in supernatants from LN cells and splenocytes challenged with myelin oligodendrocyte glycoprotein peptide were determined.ResultsFractions containing type II arabinogalactan had potent immunomodulatory activity. Specifically, the high-molecular weight sub-fraction CSP-AU1 (average of 38.5 kDa) induced NO and cytokine [interleukin (IL)-1α, -1β, -6, -10, tumor necrosis factor (TNF; designated previously as TNF-α), and granulocyte macrophage-colony stimulating factor (GM-CSF)] production by human peripheral blood mononuclear cells (PBMCs) and monocyte/macrophages. CSP-AU1-induced secretion of TNF was prevented by Toll-like receptor 4 (TLR4) antagonist LPS-RS, indicating a role for TLR4 signaling. Treatment with CSP-AU1 also induced phosphorylation of a number of MAPKs in human PBMC and activated AP-1/NF-κB. In vivo treatment of mice with CSP-AU1 and CSP-NU1 resulted in increased serum IL-6, IL-10, TNF, monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α/CCL3, and MIP-1β/CCL4. CSP-AU1 treatment of mice with EAE (50 mg/kg, i.p., daily, 13 days) resulted in significantly reduced disease severity in this experimental model of multiple sclerosis. Levels of IL-13, TNF, interferon (IFN)-γ, IL-17, and GM-CSF were also significantly decreased, whereas transforming growth factor (TGF)-β was increased in LN cells from CSP-AU1-treated EAE mice.ConclusionsPolysaccharide CSP-AU1 is a potent natural innate immunomodulator with a broad spectrum of agonist activity in vitro and immunosupressive properties after chronic administration in vivo.

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Polysaccharides from Dioscorea batatas Induce Tumor Necrosis Factor-α Secretion via Toll-like Receptor 4-Mediated Protein Kinase Signaling Pathways

Cited by 38 publications

References 29 publications

Phytochemicals Approach for Developing Cancer Immunotherapeutics

Phytochemicals Approach for Developing Cancer Immunotherapeutics

Therapeutic Applications of Herbal Medicines for Cancer Patients

Immunomodulatory activity of polysaccharides isolated from Clerodendrum splendens: Beneficial effects in experimental autoimmune encephalomyelitis

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