Polysaccharides from green sweet pepper increase the antineoplastic effect of methotrexate on mammary tumor cells

Adami, Eliana Rezende; Corso, Christopher D.; Turin-Oliveira, Natalia Mulinari; Galindo, Claudia; Milani, Letícia; Stipp, Maria Carolina; Silva, Liziane Cristine Malaquias da; Nascimento, Georgia Erdmann do; Chaves, Pedro Felipe Pereira; Chequin, Andressa; Mariott, Marihá; Silva, Luísa Mota da; Klassen, Giseli; Ramos, Edneia A.S.; Cordeiro, Lucimara M.C.; Acco, Alexandra

doi:10.1016/j.ijbiomac.2020.05.001

Cited by 8 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Moreover, DTIC is still recommended to be used as a first line therapy agent in low and middle-income countries for melanoma treatment, where no targeted therapies or immunotherapy regimens can be prescribed due to their prohibitive costs [ 6 , 7 , 8 , 41 , 42 ]. Moreover, it corroborates with the literature showing that polysaccharides may have synergistic antitumor action with chemotherapeutic agents [ 43 ]. Thus, finding an agent with an adjuvant effect to DTIC that does not cause more adverse effects and still potentiates the antitumor effect may have a positive social impact on the management of melanoma patients.…”

Section: Resultssupporting

confidence: 89%

MG-Pe: A Novel Galectin-3 Ligand with Antimelanoma Properties and Adjuvant Effects to Dacarbazine

Biscaia

Pires

Lívero

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

Melanoma is a highly metastatic and rapidly progressing cancer, a leading cause of mortality among skin cancers. The melanoma microenvironment, formed from the activity of malignant cells on the extracellular matrix and the recruitment of immune cells, plays an active role in the development of drug resistance and tumor recurrence, which are clinical challenges in cancer treatment. These tumoral metabolic processes are affected by proteins, including Galectin-3 (Gal-3), which is extensively involved in cancer development. Previously, we characterized a partially methylated mannogalactan (MG-Pe) with antimelanoma activities. In vivo models of melanoma were used to observe MG-Pe effects in survival, spontaneous, and experimental metastases and in tissue oxidative stress. Analytical assays for the molecular interaction of MG-Pe and Gal-3 were performed using a quartz crystal microbalance, atomic force microscopy, and contact angle tensiometer. MG-Pe exhibits an additive effect when administered together with the chemotherapeutic agent dacarbazine, leading to increased survival of treated mice, metastases reduction, and the modulation of oxidative stress. MG-Pe binds to galectin-3. Furthermore, MG-Pe antitumor effects were substantially reduced in Gal-3/KO mice. Our results showed that the novel Gal-3 ligand, MG-Pe, has both antitumor and antimetastatic effects, alone or in combination with chemotherapy.

show abstract

Section: Resultssupporting

confidence: 89%

MG-Pe: A Novel Galectin-3 Ligand with Antimelanoma Properties and Adjuvant Effects to Dacarbazine

Biscaia

Pires

Lívero

et al. 2022

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…In contrast, SEC is obtained by subcutaneous inoculation in the dorsal region ( Figure 1C ), paws ( Figure 1D ), or upper region of the head ( 12 - 19 ), inducing a solid tumor 14 days after inoculation ( 20 , 21 ), which is palpable from day 8 ( 16 , 18 ) and can be maintained by day 42 after inoculation ( 21 ). Tumor growth can be observed exponentially ( Figure 1E ) on a daily basis, in which the tumor mass is fibrous, firm, and well-defined ( 10 ).…”

Section: Ehrlich Tumor Types and Characteristicsmentioning

confidence: 99%

“…SEC can induce metabolic dysregulation, with a systemic proinflammatory state, skeletal muscle wasting, lipolysis, and increases in lactate dehydrogenase, creatine kinase ( 9 ), and aspartate aminotransferase ( 16 - 19 ). Cancer cachexia involves metabolic regulation in skeletal muscle after SEC cell implantation, which is linked with glucose oxidation, and changes in energy homeostasis and in insulin metabolism ( 43 ).…”

Section: Immune and Systemic Alterations Caused By Ehrlich Tumorsmentioning

confidence: 99%

“…The authors suggested that the resistance to lipid peroxidation is linked to lower levels of lipids and fatty acids and an increase in α-tocopherol per milligram lipid in AEC compared with the liver. However, in our experience with measuring levels of lipoperoxidation in SEC by the ferric-xylenol orange (FOX) method ( 47 ), the lipoperoxidation ratio can be higher in Ehrlich tumor tissue (4.9–10.0 nmol hydroperoxides/min/mg of protein) than in liver homogenate (2.6–8.0 nmol hydroperoxides/min/mg of protein; ( 8 , 16 - 19 , 44 ). Additionally, mitochondria in ascitic ECs are also resistant to lipid peroxidation mainly through the action of succinate and a glutathione-dependent mechanism ( 48 ).…”

Section: Immune and Systemic Alterations Caused By Ehrlich Tumorsmentioning

confidence: 99%

See 1 more Smart Citation

Features and applications of Ehrlich tumor model in cancer studies: a literature review

Radulski,

Stipp,

Galindo

et al. 2023

Transl Breast Cancer Res

View full text Add to dashboard Cite

Background and Objective Breast cancer is the most prevalent cancer worldwide, responsible for a large number of deaths, especially among women. Therapeutic options for breast cancer include surgery, radiotherapy, chemotherapy, hormone therapy, and immunotherapy, but further studies of the pathogenesis of this disease and new treatments are still needed. In vitro and in vivo cancer models are important research tools. Murine Ehrlich tumors are one of these models, especially for hormone-positive breast cancer. The present narrative review discusses characteristics of the Ehrlich tumor model, laboratory manipulations of Ehrlich cells (ECs), and applications in pharmacological, pathological, and translational studies. Methods This review was based on scientific articles, books, and theses on Ehrlich tumors. We searched the PubMed, SciELO, Google Scholar, Google, and Clarivate databases. Key Content and Findings Hormone-positive ECs produce solid Ehrlich carcinoma (SEC) and ascitic Ehrlich carcinoma (AEC), with different features and applications. The presence of SEC or AEC induces systemic and immunological alterations that are similar to cancer in humans, what makes this model applicable to different studies in the cancer field. Conclusions Ehrlich tumors are a relevant tool for improving our understanding of the pathogenesis of breast cancer and investigating the tumor microenvironment, side effects of therapies, and new treatment options. Despite some limitations, such as the absence of an invasive phenotype to produce metastasis, both SEC and AEC are relevant in preclinical and translational studies of breast cancer.

show abstract

“…Following a period of 7 days for acclimatization, solid tumors were induced by intramuscular inoculation of 0.2 mL of the ascetic fluid, containing approximately 2 × 10 6 of EAC cells, in the right thigh of the hind limb of each mouse [19]. When all the solid tumors have reached a size of 50-100 mm 3 , animals were randomly divided into 4 groups (10 mice per group) as followings:…”

Section: Experimental Designmentioning

confidence: 99%

Sitagliptin Potentiates the Anti-Neoplastic Activity of Doxorubicin in Experimentally-Induced Mammary Adenocarcinoma in Mice: Implication of Oxidative Stress, Inflammation, Angiogenesis, and Apoptosis

2022

View full text Add to dashboard Cite

Sitagliptin (STG) is a highly selective dipeptidyl peptidase-4 inhibitor recently used in the treatment of type 2 diabetes. The current study aimed to investigate the anti-neoplastic effect of STG alone and in combination with Doxorubicin (Dox), a known chemotherapeutic agent but with ominous side effects. After intramuscular inoculation of 2 × 106 Ehrlich tumor cells, Female Swiss mice were divided into tumor-bearing control, STG-treated, Dox-treated, and a combination of STG and Dox-treated groups. The results showed a significant reduction in the tumor growth of the treated animals in comparison with those of the positive control group with a more prominent effect in the co-treated group. Where, the anti-proliferative and apoptotic effect of STG, and its chemo-sensitizing ability, when used in combination with Dox, was mediated by modulation of oxidative stress (MDA and GSH), attenuation of tumor inflammation (IL-6 and IL-1β), and angiogenesis (VEGF), suppressing proliferation (β-catenin and cyclin-D1) and enhancement of apoptosis (survivin, p53, caspase 3). Thus, in conclusion, STG as adjunctive therapy for Dox could be a strategy for the treatment of breast cancer patients, by their ability in hindering cell proliferation and minimizing the associated oxidative and inflammatory adverse reactions.

show abstract

Polysaccharides from green sweet pepper increase the antineoplastic effect of methotrexate on mammary tumor cells

Cited by 8 publications

References 37 publications

MG-Pe: A Novel Galectin-3 Ligand with Antimelanoma Properties and Adjuvant Effects to Dacarbazine

MG-Pe: A Novel Galectin-3 Ligand with Antimelanoma Properties and Adjuvant Effects to Dacarbazine

Features and applications of Ehrlich tumor model in cancer studies: a literature review

Sitagliptin Potentiates the Anti-Neoplastic Activity of Doxorubicin in Experimentally-Induced Mammary Adenocarcinoma in Mice: Implication of Oxidative Stress, Inflammation, Angiogenesis, and Apoptosis

Contact Info

Product

Resources

About