Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats

He, Guangming; Wen, Jinkun; Hu, Jielun; Nie, Qixing; Chen, Haihong; Xiong, Tao; Nie, Shaoping; Xie, Mingyong

doi:10.1016/j.carbpol.2018.08.075

Cited by 108 publications

(45 citation statements)

References 39 publications

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“…Numerous research results have revealed that the viscosity of active physiologically macromolecules was closely related to their molecular weight. [23] For active molecule with high molecular weight, the individual molecules overlap more easily, causing intermolecular connections and forming 'junction zones', similar steady shear flow behavior was also noticed in other polysaccharides. [24] Effects of HFP on Diabetes-Related Symptoms in Diabetic Rats HFP could ameliorate typical symptoms of type 2 diabetes in rats, including lowering water intake, blood glucose and serum insulin levels, and reversing weight loss ( Figure 5A, C-E).…”

Section: Molecular Morphology Of Hfpsupporting

confidence: 52%

Physicochemical Characterization of Hizikia fusiforme Polysaccharide and Its Hypoglycemic Activity via Mediating Insulin‐Stimulated Blood Glucose Utilization of Skeletal Muscle in Type 2 Diabetic Rats

Jia

et al. 2020

Chemistry & Biodiversity

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In the current study, a functional polysaccharide fraction (HFP) was obtained from Hizikia fusiforme by ultrasound-assisted enzymatic extraction, and its structural characterization and hypoglycemic activity and potential molecular mechanism were investigated. The results indicated that HFP with high uronic acid was a heterogeneous polysaccharide composed of six monosaccharides. Congo red test explained that HFP had no triple helix conformation. AFM analysis revealed that HFP was spherical particle with flame-like aggregates and multiple strands closely arranged. Rheological analysis showed that HFP exhibited shear-thinning flow behavior. HFP significantly ameliorated diabetes-related symptoms and serum profiles and increased muscle glycogen storage in rats. HFP administration at 400 mg/kg body weight/day displayed greater advantages than metformin in controlling the levels of fasting blood glucose, triglyceride (TG), alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bile acid (TBA) of diabetic rats. Intervention of HFP up-regulated markedly the expression of AMPK-α, GLUT4, PI3K and Akt in skeletal muscle of diabetic rats at the mRNA and protein levels, revealing hypoglycemic effects of HFP may be related closely to improving insulin resistance and mitochondrial function of skeletal muscle.

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Section: Molecular Morphology Of Hfpsupporting

confidence: 52%

Physicochemical Characterization of Hizikia fusiforme Polysaccharide and Its Hypoglycemic Activity via Mediating Insulin‐Stimulated Blood Glucose Utilization of Skeletal Muscle in Type 2 Diabetic Rats

Jia

et al. 2020

Chemistry & Biodiversity

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“…The difference was statistically significant ( p < .05). Accumulating evidence suggested that non‐digestible carbohydrates could be fermented by bacteria in the colon, contributing to the SCFAs production (Gao et al, ; Liu et al, ). Yang et al reported that the production of butyric acid was mainly resulted from the fermentation of galactose, mannose and galacturonic acid (Yang, Martínez, Walter, Keshavarzian, & Rose, ).…”

Section: Resultsmentioning

confidence: 99%

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

Wang

et al. 2020

Animal Physiology Nutrition

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The structural characteristics of a novel Flammulina velutipes polysaccharide (FVP2) were explored in this study. Besides, immunomodulatory activities of FVP2 on RAW 264.7 cell and its impact on gut microbiota in rats were investigated. FVP2 has a molecular weight of 18.3 kD, and its main components include galactose (19.96%), glucose (60.66%) and mannose (19.38%). By NMR analysis, the main‐chain structure consisted of (1 → 3)‐linked‐β‐D‐Gal, (1 → 6) ‐linked‐β‐D‐Gal, (1 → 6)‐linked‐α‐D‐Glc and (1 → 3,6)‐linked‐α‐D‐Man was identified. Results of the in vitro assays on RAW 264.7 murine macrophage cells showed FVP2 could significantly up‐regulate the expression of NO, TNF‐α and IL‐6. FVP2 was intragastrically administered to rats for 2 weeks. Compared with the control group, two caecal short‐chain fatty acids (SCFAs) concentration (isobutyric acid and butyric acid) and the abundance of beneficial microbiota of the FVP2‐treated group were significantly increased (p < .05) respectively. The results demonstrated that FVP2 could effectively enhance the level of butyric acid and increase beneficial gut microbiota, which could improve the intestinal barrier function and maintain the intestinal mucosal integrity, suggesting that FVP2 could potentially be an immunomodulators or a functional food to promote intestinal health.

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“…The dried bodies of WuGuChong were purchased in a traditional Chinese medicine market and identified according to the standards in "Chinese Materia Medica Standards". The PEW was isolated according to water extraction and alcohol precipitation methods as previous reported [24]. The Sevage method and DEAE iron-exchanged chromatography method were used for deproteinization and purification of polysaccharides.…”

Section: Pew Preparationmentioning

confidence: 99%

Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota

Wang

Zhong

Yu³

et al. 2020

Nutr Metab (Lond)

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Background: Obesity is a severe public health threat worldwide. Emerging evidence suggests that gut microbiota dysbiosis is closely associated with obesity and its related metabolic complications. The aim of the present study is to investigate the effects of polysaccharide extracted from WuGuChong (PEW) on high-fat diet (HFD)-induced obesity, and the potential mechanisms involving modulation of the gut microbiota composition. Methods: Mice were fed a normal chow diet and a high-fat diet with or without PEW (300 mg/kg/day) by oral gavage for 8 weeks. Body weight, obesity-related metabolic disorders, and gut microbiota were examined at the end of the experiment. Results: PEW supplementation reduces body weight, adipose hypertrophy, liver steatosis, insulin resistance and systemic inflammation in HFD-fed mice, as well as maintains intestinal epithelium integrity. High-throughput 16S rRNA analysis demonstrates that PEW supplementation alters the composition of gut microbiota. The Firmicutes to Bacteroidetes ratio and the relative abundance of Proteobacteria are increased in HFD-fed mice, which are reversed by PEW supplementation to approximately the control levels. Conclusions: Our results suggest that PEW may be used as a bioactive ingredient to prevent obesity and its related metabolic disorders by modulating the composition of gut microbiota.

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Polysaccharide from fermented Momordica charantia L. with Lactobacillus plantarum NCU116 ameliorates type 2 diabetes in rats

Cited by 108 publications

References 39 publications

Physicochemical Characterization of Hizikia fusiforme Polysaccharide and Its Hypoglycemic Activity via Mediating Insulin‐Stimulated Blood Glucose Utilization of Skeletal Muscle in Type 2 Diabetic Rats

Physicochemical Characterization of Hizikia fusiforme Polysaccharide and Its Hypoglycemic Activity via Mediating Insulin‐Stimulated Blood Glucose Utilization of Skeletal Muscle in Type 2 Diabetic Rats

A novel polysaccharide isolated from Flammulina velutipes, characterization, macrophage immunomodulatory activities and its impact on gut microbiota in rats

Polysaccharide extracted from WuGuChong reduces high-fat diet-induced obesity in mice by regulating the composition of intestinal microbiota

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