Polysaccharide extracted from Atractylodes macrocephala Koidz (PAMK) induce apoptosis in transplanted H22 cells in mice

Feng, Yingying; Ji, Haiyu; Dong, Xiaodan; Yu, Juan; Liu, Anjun

doi:10.1016/j.ijbiomac.2019.06.059

Cited by 38 publications

(21 citation statements)

References 37 publications

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“…In cancers, Atractylodes macrocephala Koidz. has been demonstrated to play a cytotoxic and anti-tumor effect by arresting tumor cells in the S phase 28 , 29 . Scutellaria barbarta D.Don contains a variety of chemical components, including flavonoids, diterpenoids, alkaloids, steroids, and polysaccharides.…”

Section: Discussionmentioning

confidence: 99%

Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer

Cheng¹,

Chen²,

Chen³

2020

J. Cancer

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Precancerous lesions are the intermediate stage in the development of liver cancer from cirrhosis. Early intervention measures can effectively prevent the occurrence of liver cancer and prolong the lives of patients, resulting in greater economic effects. Erzhu Jiedu decoction (EJD) is a semiempirical formula that is used in the treatment of cirrhosis and liver cancer according to the academic philosophy of “Preventive treatment of disease” and has achieved good curative effects in clinical practice. The purpose of this study was to investigate the effect of EJD on liver precancerous lesions induced by diethylnitrosamine (DEN) in rats. The results showed that EJD improved the general conditions (body weight, ALT, AST, and GGT) and reduced the number of precancerous lesions in the rat model. Notably, the medium dose of EJD (1.05 g/kg) had better treatment effects than the low dose of EJD, and the high dose of EJD did not further improve the liver lesions compared to the medium dose of EJD. Moreover, EJD effectively reduced the DEN-induced GST-Pi, AFP, CK19, c-Myc, and Ki67 protein expression in liver precancerous tissues. Interestingly, EJD significantly reduced YAP and TAZ mRNA expression in the liver precancerous lesions. Collectively, EJD protects against in the initiation of liver cancer and the regulation of c-Myc and Hippo signaling pathways may be the underlying mechanism.

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Section: Discussionmentioning

confidence: 99%

Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer

Cheng¹,

Chen²,

Chen³

2020

J. Cancer

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“…Consequently, it is essential to find a safe and effective natural anticancer drug with low toxicity. It was well‐known that the antitumor effects of bioactive substances are often studied by establishing a mouse model of H22 liver cancer and using cyclophosphamide (CTX) or 5‐Fu as a positive control group [14–16] …”

Section: Introductionmentioning

confidence: 99%

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Niu

Liu

et al. 2021

Chemistry & Biodiversity

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In the present study, a novel cold water‐soluble polysaccharide fraction (LGP) with the average molecular weight of 1.78×106 Da was extracted and purified from Leucopaxillus giganteus and its primary structure as well as in vivo antitumor activity was evaluated. The monosaccharide composition of LGP was determined by ion chromatography to be galactose, xylose, glucose and fucose in a molar ratio of 2.568 : 1.209 : 1 : 0.853. Its backbone was composed of α‐D‐Glu, α‐D‐Xyl, α‐D‐Gal and α‐L‐Fuc. The results of in vivo antitumor experiment demonstrated that LGP could effectively protect immune organs, has excellent antitumor activity, and inhibit the proliferation of H22 solid tumors in a dose‐dependent manner. By analyzing Annexin V‐FITC/PI staining, cell cycle and mitochondrial membrane potential detection assay, we concluded that LGP induced apoptosis of H22 cells via S phase arrest and mitochondria‐mediated apoptotic pathway. Our results could provide valuable information for the potential application of LGP as an anti‐hepatoma agent.

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“…The mice were randomly divided into the following five groups: (1) Normal group (2) Model group (3) CTX group (4) 150 mg/kg AASP group and (5) 300 mg/kg AASP group [41] . H22 cells (1×10 6 ) were suspended in 0.2 ml of saline and inoculated subcutaneously into the right armpits of group (2–5).…”

Section: Methodsmentioning

confidence: 99%

Structural Characterization of an Alkali‐Soluble Polysaccharide from Angelica sinensis and Its Antitumor Activity in Vivo

Zhao

Feng

Xue

et al. 2021

Chemistry & Biodiversity

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A novel alkali‐soluble polysaccharide (AASP) was isolated from Angelica sinensis (Oliv.) Diels under aqueous alkali treatment, and its structural characterization and antitumor activity in Vivo were evaluated in present study. Results of HPGPC and IC revealed that AASP was a neutral polysaccharide containing Ara, Gal and Glc in the mole ratio of 1.00 : 2.26 : 24.43, with the average molecular weight of 4.7 kDa. Periodate oxidation, Smith degradation, methylation, FT‐IR, and NMR analyses further demonstrated that a preliminary structure of AASP was proposed as follows: (1→3)‐linked arabinose, (1→6)‐linked galactose, and (1→), (1→4), (1→6), (1→3,6)‐linked glucose with α‐ and β‐configuration. In Vivo antitumor assays, AASP exhibited prominent antitumor effects on H22 hepatoma cells with an inhibitory ratio of 48.57 % and effectively protected thymuses and spleens of tumor‐bearing mice. Besides, AASP displayed a proliferation stimulating activity of immunocytes (splenocytes, peritoneal macrophages and natural killer cells), and an auxo‐action for cytokines release (TNF‐α, IL‐2 and IFN‐γ), leading to the apoptosis of H22 solid tumors cells via G0/G1 phase arrested. The above data demonstrated that AASP holds great application potential to be a safe and effective antitumor supplement in the future.

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Polysaccharide extracted from Atractylodes macrocephala Koidz (PAMK) induce apoptosis in transplanted H22 cells in mice

Cited by 38 publications

References 37 publications

Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer

Erzhu Jiedu decoction ameliorates liver precancerous lesions in a rat model of liver cancer

The Structural Characterization of a Novel Water‐Soluble Polysaccharide from Edible Mushroom Leucopaxillus giganteus and Its Antitumor Activity on H22 Tumor‐Bearing Mice

Structural Characterization of an Alkali‐Soluble Polysaccharide from Angelica sinensis and Its Antitumor Activity in Vivo

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