Polysaccharide‐Coated PCL Nanofibers for Wound Dressing Applications

Abstract

Polysaccharide-based nanofibers with a multilayered structure are prepared by combining electrospinning (ESP) and layer-by-layer (LBL) deposition techniques. Charged nanofibers are firstly prepared by electrospinning poly(ε-caprolactone) (PCL) with a block-copolymer bearing carboxylic acid functions. After deprotonation of the acid groups, the layer-by-layer deposition of polyelectrolyte polysaccharides, notably chitosan and hyaluronic acid, is used to coat the electrospun fibers. A multilayered structure is achieved by alternating the deposition of the positively charged chitosan with the deposition of a negatively charged polyelectrolyte. The construction of this multilayered structure is followed by Zeta potential measurements, and confirmed by observation of hollow nanofibers resulting from the dissolution of the PCL core in a selective solvent. These novel polysaccharide-coated PCL fiber mats remarkably combine the mechanical resistance typical of the core material (PCL)-particularly in the hydrated state-with the surface properties of chitosan. The control of the nanofiber structure offered by the electrospinning technology, makes the developed process very promising to precisely design biomaterials for tissue engineering. Preliminary cell culture tests corroborate the potential use of such system in wound healing applications.

Preparation of active 3D film patches via aligned fiber electrohydrodynamic (EHD) printing

“…Then, 1 ml of supernatant was removed to determine the concentration of drug released using UV spectrophotometry and replaced with an equal volume of fresh release medium. The cumulative release of TE-HCL (α) determined using equation (3)3450:…”

“…PCL-drug composite nanofiber mats have been investigated as controlled release wound dressing materials34. Polyvinyl pyrrolidone (PVP) has been used as a rapidly dissolving matrix carrier for actives in several drug delivery system formulations35.…”

Hierarchical Self‐Assembly of Poly(Urethane)/Poly(Vinylidene Fluoride‐co‐Hexafluoropropylene) Blends into Highly Hydrophobic Electrospun Fibers with Reduced Protein Adsorption Profiles

“…When used as implants for medical purposes, cell material interactions are governed by chemical and physical surface properties that trigger initial protein adsorption, and regulate further downstream signals for cell adhesion, cell proliferation, and/or differentiation . Typically, improved biointerfaces and surface functionalization are addressed by post‐treatment such as plasma coating processes, layer by layer assembly, photochemical surface modifications, wet chemical treatment, or gas phase induced coating processes . Recently, fluorine‐functionalized surfaces have gained increasing interest in the field of biomedical applications due to their outstanding properties of hydrophobicity and oleophilicity, chemical resistance, low friction coefficient, and mechanical stability.…”

Making Nonwoven Fibrous Poly(ε‐caprolactone) Constructs for Antimicrobial and Tissue Engineering Applications by Pressurized Melt Gyration

“…This semi‐crystalline polymer has a low melting point (60 °C) and a glass transition temperature (−60 °C) and therefore it could be fabricated easily into any shape and size . The superior rheological properties and mechanical properties of PCL have attracted interest in using this polymer in tissue engineering scaffolds, blood vessels, vascular grafts, and wound healing mats . The ability to blend PCL with other natural polymers such as bacterial cellulose and cellulose acetate also makes it an excellent candidate for wound dressing scaffolds .…”