Polyplex‐mediated gene transfer and cell cycle: effect of carrier on cellular uptake and intracellular kinetics, and significance of glycosaminoglycans

Abstract

The data obtained indicates that no single parameter (pinocytosis, cell surface GAGs, nuclear uptake) solely accounts for the differential pDNA uptake or expression during cell cycle, and that the main difference in PLL- and PEI-mediated transfections seems to be at the nuclear level.

Controlled complexation of plasmid DNA with cationic polymers: Effect of surfactant on the complexation and stability of the complexes

“…Our results are somewhat contradictory with the results reported in the literature: PEI complexes have shown to be less resistant to the effects of GAGs compared to PLL complexes [34,35]. The different results may be explained by the polydispersity of our PLL/DNA complexes.…”

Chemistry specificity of DNA–polycation complex salt response: a simulation study of DNA, polylysine and polyethyleneimine

“…The structure also leads to to bulkier complexes compared to PEI, which has been indicated experimentally 11,19 as well.…”

“…Out of the two, PEI is often considered more promising due to its ability to change its protonation state under physiological pH range. A host of both experimental 4,[17][18][19][20] and computational work 21-27 on these complexes exists. That said, response of the DNA-polycation complexes to increasing concentrations of monovalent and divalent ions has not been compared earlier via simulations.…”

Nuclear Inclusion of Nontargeted and Chromatin-Targeted Polystyrene Beads and Plasmid DNA Containing Nanoparticles

“…These attempts at improving the transport of DNA to the nucleus have however met with limited success. 9,11,[17][18][19][20][21][22][23][24] . Also, cell division had a positive influence on the nuclear inclusion of colloidal gold particles 25 .…”