Polyphosphate polymers during early embryogenesis of Periplaneta americana

Gomes, Fabio M.; Ramos, Isabela; Motta, Laura Maria Goretti da; Miranda, Kildare; Santiago, Marcelo F.; Souza, Wanderley de; Machado, Ednildo A.

doi:10.1016/j.jinsphys.2008.07.018

Cited by 11 publications

(17 citation statements)

References 48 publications

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“…Furthermore, inositol polyP has been suggested to have numerous roles in signaling and developmental process during germination, including gene expression (Shears 2004;Bowen et al 2007). The globoid is suggested to be involved in embryo development during seed germination, like the acidocalcisome-like organelle found recently in the egg yolks of insects (Gomes et al 2008). In insect eggs, the acidic environment of the matrix induces polyP synthesis, which promotes Ca storage.…”

Section: Polyp In Vascular Plants: Insights Into Embryo Developmentmentioning

confidence: 92%

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Recent Discoveries on the Roles of Polyphosphates in Plants

Seufferheld

Curzi

2010

Plant Mol Biol Rep

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Polyphosphate (polyP) has been abundant on Earth long before life began. In living organisms, polyphosphate can be stored in electron-dense granules that have long been referred to as volutin granules. After having being ignored for a long time, this polymer has proven to have numerous unsuspected functions in living organisms and is receiving more attention. The ubiquitous presence of polyP in nature and its structural and physico-chemical characteristics appear to be the drivers for the evolutionary selection of this versatile molecule as a component of cellular processes. Most of our knowledge about the functions of polyP comes from the microbial world. PolyP can influence the transcription and translation of specific genes, act as a metabolic regulator, provide an alternative source of energy, and modulate several stress responses. In plant systems, although the presence of polyP has long been known, its roles in cellular processes have begun to emerge only recently. The purpose of this mini-review is to provide a brief overview of the proposed biological roles of polyphosphates in plants and their roles in microbial/plant interactions.

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Section: Polyp In Vascular Plants: Insights Into Embryo Developmentmentioning

confidence: 92%

“…In insect eggs, the acidic environment of the matrix induces polyP synthesis, which promotes Ca storage. This process acts as a dynamic system for P i and Ca homeostasis (Gomes et al 2008). It is possible that a similar mechanism operates in seeds.…”

Section: Polyp In Vascular Plants: Insights Into Embryo Developmentmentioning

confidence: 99%

Recent Discoveries on the Roles of Polyphosphates in Plants

Seufferheld

Curzi

2010

Plant Mol Biol Rep

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“…23 DAPI-PolyP interaction was shown to be remarkably specific and to generate a proper quantum yield for microscopic observation. 21,24-26 Nevertheless, reports concerning the feasibility of using DAPI as a PolyP probe have remained fragmented among several publications that suggested a number of possible routines for DAPI-PolyP labeling and visualization. 23,27,28 …”

Section: Introductionmentioning

confidence: 99%

New insights into the in situ microscopic visualization and quantification of inorganic polyphosphate stores by 4',6-diamidino-2-phenylindole (DAPI)-staining

et al. 2013

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Inorganic polyphosphate (PolyP) is a biological polymer that plays important roles in the cell physiology of both prokaryotic and eukaryotic organisms. Among the available methods for PolyP localization and quantification, a 4’,6-diamidino-2-phenylindole(DAPI)-based assay has been used for visualization of PolyP-rich organelles. Due to differences in DAPI permeability to different compartments and/or PolyP retention after fixation, a general protocol for DAPI-PolyP staining has not yet been established. Here, we tested different protocols for DAPI-PolyP detection in a range of samples with different levels of DAPI permeability, including subcellular fractions, free-living cells and cryosections of fixed tissues. Subcellular fractions of PolyP-rich organelles yielded DAPI-PolyP fluorescence, although those with a complex external layer usually required longer incubation times, previous aldehyde fixation and/or detergent permeabilization. DAPI-PolyP was also detected in cryosections of OCT-embedded tissues analyzed by multiphoton microscopy. In addition, a semi-quantitative fluorimetric analysis of DAPI-stained fractions showed PolyP mobilization in a similar fashion to what has been demonstrated with the use of enzyme-based quantitative protocols. Taken together, our results support the use of DAPI for both PolyP visualization and quantification, although specific steps are suggested as a general guideline for DAPI-PolyP staining in biological samples with different degrees of DAPI and PolyP permeability.

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“…The presence of poly P stored in acidocalcisomes has been extensively reported in different models [15], [23], and changes in its levels according to varied extracellular stimuli were observed [35]. Interestingly, the levels of poly P in Rhodnius acidocalcisomes tend to decrease slightly before the beginning of yolk mobilization, and a decrease in the levels of this polymer was already observed in eggs of the cattle tick Boophilus microplus [61] and the cockroach P. americana [37]. These data suggest that poly P might be widely used as Pi source for growing embryos, mainly in invertebrates, that do not store massively phosphorylated yolk proteins as phosvitins, known in vertebrate models [62].…”

Section: Discussionmentioning

confidence: 88%

“…Total Poly P was extracted as described before by Gomes et al (2008) [37]. Acidocalcisome fractions were resuspended in water and sonicated (probe sonication, 30% amplitude, three cycles of 20 s).…”

Section: Methodsmentioning

confidence: 99%

Acidocalcisomes as Calcium- and Polyphosphate-Storage Compartments during Embryogenesis of the Insect Rhodnius prolixus Stahl

et al. 2011

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BackgroundThe yolk of insect eggs is a cellular domain specialized in the storage of reserve components for embryo development. The reserve macromolecules are stored in different organelles and their interactions with the embryo cells are mostly unknown. Acidocalcisomes are lysosome-related organelles characterized by their acidic nature, high electron density and large content of polyphosphate bound to several cations. In this work, we report the presence of acidocalcisome-like organelles in eggs of the insect vector Rhodnius prolixus.Methodology/Principal findingsCharacterization of the elemental composition of electron-dense vesicles by electron probe X-ray microanalysis revealed a composition similar to that previously described for acidocalcisomes. Following subcellular fractionation experiments, fractions enriched in acidocalcisomes were obtained and characterized. Immunofluorescence showed that polyphosphate polymers and the vacuolar proton translocating pyrophosphatase (V-H+-PPase, considered as a marker for acidocalcisomes) are found in the same vesicles and that these organelles are mainly localized in the egg cortex. Polyphosphate quantification showed that acidocalcisomes contain a significant amount of polyphosphate detected at day-0 eggs. Elemental analyses of the egg fractions showed that 24.5±0.65% of the egg calcium are also stored in such organelles. During embryogenesis, incubation of acidocalcisomes with acridine orange showed that these organelles are acidified at day-3 (coinciding with the period of yolk mobilization) and polyphosphate quantification showed that the levels of polyphosphate tend to decrease during early embryogenesis, being approximately 30% lower at day-3 compared to day-0 eggs.ConclusionsWe found that acidocalcisomes are present in the eggs and are the main storage compartments of polyphosphate and calcium in the egg yolk. As such components have been shown to be involved in a series of dynamic events that may control embryo growth, results reveal the potential involvement of a novel organelle in the storage and mobilization of inorganic elements to the embryo cells.

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Polyphosphate polymers during early embryogenesis of Periplaneta americana

Cited by 11 publications

References 48 publications

Recent Discoveries on the Roles of Polyphosphates in Plants

Recent Discoveries on the Roles of Polyphosphates in Plants

New insights into the in situ microscopic visualization and quantification of inorganic polyphosphate stores by 4',6-diamidino-2-phenylindole (DAPI)-staining

Acidocalcisomes as Calcium- and Polyphosphate-Storage Compartments during Embryogenesis of the Insect Rhodnius prolixus Stahl

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