Polyphenols from the hydroalcoholic extract of Arbutus unedo living in a monospecific Mediterranean woodland

Fiorentino, Antonio; Castaldi, Simona; D’Abrosca, Brigida; Natale, Angela; Carfora, Anna; Messere, Anna; Monaco, Pietro

doi:10.1016/j.bse.2007.04.005

Cited by 45 publications

(41 citation statements)

References 8 publications

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“…They are released into the environment in large quantities by means of volatilization, leaching, decomposition of residues and root exudation, the most common ones including phenolics, alkaloids and terpenoids (Putnam 1988;Bertin et al 2003). Several works reported a negative effect of polyphenols and terpenes on net nitrification in incubation studies (Rice and Pancholy 1973;Thibault et al 1982;Baldwin et al 1983;Howard and Howard 1991;White 1991;Paavolainen et al 1998;Erickson et al 2000). These results were explained, in part, by a direct inhibiting effect of the chemical compounds on mineralization and nitrification activity, but also by an increase of N immobilization (McCarty and Bremner 1986;Northup et al 1995;Schimel et al 1996).…”

Section: Introductionmentioning

confidence: 97%

Inhibition of net nitrification activity in a Mediterranean woodland: possible role of chemicals produced by Arbutus unedo

et al. 2008

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Nitrification is a key biological process for the control of soil NO 3 − availability and N losses from terrestrial ecosystems. The study investigates the causes for the absence of net nitrification activity in the soil of a Mediterranean monospecific woodland of Arbutus unedo, focusing in particular on the possible role of chemicals produced by this plant. The mineral N pool, net rates of mineralization and nitrification were measured in the soil top 10 cm over 18 months. Raw extracts of leaves and roots of Arbutus unedo and soil underneath Arbutus plant canopy were purified using chromatographic techniques and the structure of chemicals was defined using spectroscopic and spectrometric methods. Leaf extracts (raw, aqueousand organic fractions) were tested for their toxicity on net nitrification, using a test soil. Field and laboratory incubations showed soil NO 3 − concentration below the detection limit over the whole study period, despite the significant NH 4 + availability. Toxicity tests indicated that more than 400 μg of extract g −1 dry soil were needed to have more than 50% reduction of net NO 3 − production. Gallocatechin and catechin were among the most abundant chemicals in the extracts of leaves, roots and soil. Their soil concentration was significantly higher than the annual calculated input via leaf litter, and it was in the range of toxic concentrations, as deduced from the doseresponse curve of the toxicity test. Data support the hypothesis that plant produced chemicals might be involved in the limited net nitrate production in this Mediterranean woodland.

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Section: Introductionmentioning

confidence: 97%

Inhibition of net nitrification activity in a Mediterranean woodland: possible role of chemicals produced by Arbutus unedo

et al. 2008

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“…(bearberry), Vaccinium vitis-idaea L. (lingonberry)], while less often in some other species such as marjoram or Origanum majorana L. from the Lamiaceae family (1,2). This phenolic glycoside is a secondary plant metabolite that forms as a plant's response against infectious diseases and environmental stress conditions (e.g.…”

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confidence: 99%

“…unedo, an evergreen wild shrub that grows in the Mediterranean region (10), contains many pharmacologically relevant polyphenols and one of its main phenolic compounds is arbutin (1,11). A. unedo leaf infusions are known to have diuretic, astringent, and uroantiseptic properties and are used in folk medicine for treatment of hypertension, diabetes, and inflammation (12)(13)(14)(15).…”

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confidence: 99%

Quantitative analysis of arbutin and hydroquinone in strawberry tree (Arbutus unedo L., Ericaceae) leaves by gas chromatography-mass spectrometry / Kvantitativna analiza arbutina i hidrokinona u listovima obične planike (Arbutus unedo L., Ericaceae) plinskokromatografskom metodom uz detekciju masenim spektrometrom

Jurica

Karačonji

Šegan

et al. 2015

Archives of Industrial Hygiene and Toxicology

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The phenolic glycoside arbutin and its metabolite with uroantiseptic activity hydroquinone occur naturally in the leaves of various medicinal plants and spices. In this study, an extraction procedure coupled with gas chromatography-mass spectrometry (GC-MS) was developed to determine arbutin and hydroquinone content in strawberry tree (Arbutus unedo L., Ericaceae) leaves. The method showed good linearity (R 2 >0.9987) in the tested concentration range (0.5-200 µg mL), as well as good precision (RSD<5 %), analytical recovery (96.2-98.0 %), and sensitivity (limit of detection=0.009 and 0.004 µg mL -1 for arbutin and hydroquinone, respectively). The results obtained by the validated GC-MS method corresponded well to those obtained by high performance liquid chromatography (HPLC) method. The proposed method was then applied for determining arbutin and hydroquinone content in methanolic leaf extracts. The amount of arbutin in the leaves collected on the island of Koločep (6.82 mg g -1 dry weight) was found to be higher (t paired =43.57, t c =2.92) in comparison to the amount of arbutin in the leaves collected on the island of Mali Lošinj (2.75 mg g -1 dry weight). Hydroquinone was not detected in any of the samples. The analytical features of the proposed GC-MS method demonstrated that arbutin and hydroquinone could be determined alternatively by gas chromatography. Due to its wide concentration range, the method could also be suitable for arbutin and hydroquinone analysis in leaves of other plant families (Rosaceae, Lamiaceae, etc.).

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“…The strawberry leaves also contain lipids and vitamin E (Chevolleau et al, 1992;Fiorentino et al, 2007;Kıvçak and Mert, 2001;Pabuçcuoğlu et al, 2003).…”

Section: Intracellular Antimycobacterial Activity Of Ethanolic Plant mentioning

confidence: 99%

“…Previous studies indicate that leaves of Arbutus unedo contain several phenolic compounds like tannins, flavonoids, phenolic glycosides, lipids and vitamin E (Chevolleau et al, 1992;Fiorentino et al, 2007;Kıvçak and Mert, 2001;Pabuçcuoğlu et al, 2003). This melliferous plant has a medicinal properties, namely the leaves and bark (Rocha-Afonso, 1991).…”

Section: Introductionmentioning

confidence: 99%

Extra- and intracellular antimycobacterial activity of Arbutus unedo L.

Ouarti¹,

Haouat²,

Sqalli³

et al. 2012

Afr. J. Microbiol. Res.

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This study aims to evaluate extra-and intracellullar antimycobacterial activity of Arbutus unedo L. leaves-extracts. We tested the aqueous and ethanol extracts of Arbutus leaves effect against three mycobacteria growth, and showed that aqueous extract and the part I of ethanol extract have a remarkable extracellular antimycobacterial activity. The MIC (Minimum inhibitory concentration) of the part I of ethanol extract is 5.59 ± 0.69 mg/ml for Mycobacterium aurum A + and 6.02 ± 0.76 mg/ ml for Mycobacterium smegmatis MC 2 and Mycobacterium bovis PPI. The part I of arbutus ethanol-extract, used at 6.02 ± 0.76 mg/ml, has a bactericidal effect on M. smegmatis MC 2 located within the rat peritoneal macrophages.

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Polyphenols from the hydroalcoholic extract of Arbutus unedo living in a monospecific Mediterranean woodland

Cited by 45 publications

References 8 publications

Inhibition of net nitrification activity in a Mediterranean woodland: possible role of chemicals produced by Arbutus unedo

Inhibition of net nitrification activity in a Mediterranean woodland: possible role of chemicals produced by Arbutus unedo

Extra- and intracellular antimycobacterial activity of Arbutus unedo L.

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