Polyphenols content of selected medical plants and food supplements present at Bulgarian market

Koleva, Petya; Tsanova-Savova, Silvia; Paneva, Slaveyka; Velikov, Stefan; Savova, Zaharina

doi:10.3897/pharmacia.68.e71460

Cited by 5 publications

(3 citation statements)

References 20 publications

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“…The phenolic compounds were assessed by the HPLC method described by Filip et al [21]. Several studies have demonstrated that high-performance liquid chromatography (HPLC) is the most efficient technique for the qualitative and quantitative determination of polyphenolic compounds in plants, due to its high selectivity and precision [12,[22][23][24][25][26][27][28].…”

Section: Quantitative Analysis Of Phenolic Compounds In Elderflowersmentioning

confidence: 99%

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

et al. 2022

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The use of gentamicin (GM) is limited due to its nephrotoxicity mediated by oxidative stress. This study aimed to evaluate the capacity of a flavonoid-rich extract of Sambucus nigra L. elderflower (SN) to inhibit lipoperoxidation in GM-induced nephrotoxicity. The HPLC analysis of the SN extract recorded high contents of rutin (463.2 ± 0.0 mg mL−1), epicatechin (9.0 ± 1.1 µg mL−1), and ferulic (1.5 ± 0.3 µg mL−1) and caffeic acid (3.6 ± 0.1 µg mL−1). Thirty-two Wistar male rats were randomized into four groups: a control group (C) (no treatment), GM group (100 mg kg−1 bw day−1 GM), GM+SN group (100 mg kg−1 bw day−1 GM and 1 mL SN extract day−1), and SN group (1 mL SN extract day−1). Lipid peroxidation, evaluated by malondialdehyde (MDA), and antioxidant enzymes activity—superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPX)—were recorded in renal tissue after ten days of experimental treatment. The MDA level was significantly higher in the GM group compared to the control group (p < 0.0001), and was significantly reduced by SN in the GM+SN group compared to the GM group (p = 0.021). SN extract failed to improve SOD, CAT, and GPX activity in the GM+SN group compared to the GM group (p > 0.05), and its action was most probably due to the ability of flavonoids (rutin, epicatechin) and ferulic and caffeic acids to inhibit synthesis and neutralize reactive species, to reduce the redox-active iron pool, and to inhibit lipid peroxidation. In this study, we propose an innovative method for counteracting GM nephrotoxicity with a high efficiency and low cost, but with the disadvantage of the multifactorial environmental variability of the content of SN extracts.

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Section: Quantitative Analysis Of Phenolic Compounds In Elderflowersmentioning

confidence: 99%

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

et al. 2022

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“…All measurements in the present study were performed in triplicate and the methods were validated, according ISO 17025 requirements [24].…”

Section: Hplc Determination Of (+)-Catechin (-)-Epicatechin and Rutinmentioning

confidence: 99%

Comparative Evaluation of the Content of Antioxidant Polyphenolic Compounds in Selected Bulgarian Medicinal Plants

Tsanova-Savova¹,

Velikov

Paneva³

et al. 2022

Acta Medica Bulgarica

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Polyphenols are strong antioxidants of plant origin that exhibit protective role against the development of several chronic degenerative diseases. Quantitative data on the content of polyphenolic antioxidants in Bulgarian medicinal plants are sporadic and unsystematic. The aim of the study is to determine polyphenols content in series of extracts, infusions and decocts of selected Bulgarian medicinal plants and fruits and to compare their polyphenolic profile. Materials and Methods: In the present study 18 Bulgarian medicinal plants and fruits have been analyzed for Total Phenolic and Total Flavonoids content, along with HPLC analysis for their level of the individual flavonoids (+)-Catechin, (-)-Epicatechin and Rutin. Comparative evaluation of the content of antioxidant polyphenolic compounds was performed by hierarchical cluster analysis. The results show that infusions of Hawthorn (Crategus monogyna) flowers have the highest values of Total Phenolic and Total Flavonoids content, forming a separate Cluster in the analysis of the Agglomeration schedule coefficients. Within fruits, data for Total Phenolics and Total Flavonoids content are more homogenic as the cluster analysis shows distribution of 6 elements in a high mean value group. Elderberry (Sambicus nigra) flowers and fruits are the richest source of Rutin in the selected plants studied. Conclusion: In the present study new data for polyphenolic content in selected Bulgarian medicinal plants and fruits are presented and their degree of similarity in methanolic extracts, infusions and decocts have been assessed in order to provide new data for development of effective antioxidant medicinal plant compositions.

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“…За определяне на общите феноли е използван реагент на Фолин-Чикалто, окисляващ фенолатите до син комплекс, който се определя спектрофотометрично при дължина на вълната λ=750 nm (8). Резултатите са изразени като галова киселина еквивалент (mg GAE/100 ml).…”

Section: определяне на общи фенолиunclassified

Comparative Characteristics of Total Phenols in Water Infusions of Bulgarian Medicinal Plants

Tsanova-Savova,

Paneva,

Ivanova

et al. 2022

vmf

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Polyphenols content of selected medical plants and food supplements present at Bulgarian market

Cited by 5 publications

References 20 publications

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

A Flavonoid-Rich Extract of Sambucus nigra L. Reduced Lipid Peroxidation in a Rat Experimental Model of Gentamicin Nephrotoxicity

Comparative Evaluation of the Content of Antioxidant Polyphenolic Compounds in Selected Bulgarian Medicinal Plants

Comparative Characteristics of Total Phenols in Water Infusions of Bulgarian Medicinal Plants

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