Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method

Miłek, Michał; Marcinčáková, Dana; Legáth, Jaroslav

doi:10.3390/molecules24061025

Cited by 36 publications

(25 citation statements)

References 40 publications

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“…Lower concentrations can support proliferation, while higher concentrations cause negative effects. Similar relationships have been previously observed in other experiments, for example: water-acetone extracts of dandelion ( Taraxacum officinale ) on the porcine epithelial renal cell line [ 37 , 38 ], anthocyanin-rich extracts of Vaccinium angustifolium on HepG2 cells [ 39 ] and Oenothera paradoxa extracts on Caco-2 cells [ 40 ]. Additional tests would be required to elucidate the mechanism of action of the tested extracts on cells.…”

Section: Resultssupporting

confidence: 85%

“…The system gives information about behaviour of cells, their adherence, proliferation and change in morphology, and expresses them in a dimensionless unit: cell index (CI). The greater the CI, the greater the adhesion of cells [ 36 , 37 ]. Introducing the effector to cell culture induces the response of the target adherent cells and the corresponding activity (loss of adherence), which can be detected with great sensitivity and precision.…”

Section: Resultsmentioning

confidence: 99%

“…Changes are expressed by a dimensionless unit cell index (CI) and recorded in curves. This method was described in various studies [ 37 , 38 , 62 , 63 ]. Initial cell seeding density was optimised using the xCELLigence system prior to the experiment.…”

Section: Methodsmentioning

confidence: 99%

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Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

et al. 2020

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Plants from Asteraceae family are widely used for their therapeutic effects in the treatment of gastrointestinal diseases, but the consequences of excessive intake still need to be studied. The aims of this study were the evaluation of cytotoxicity, measurement of antioxidant properties and determination of polyphenolic profile of Tanacetum vulgare L. (tansy), Achillea millefolium L. (yarrow) and Solidago gigantea Ait. (goldenrod) ethanolic extracts. The cytotoxicity of extracts was monitored by xCELLigence system in real time by using porcine intestinal epithelial cell line (IPEC-1) and by measurement of changes in metabolic activity ((3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) (MTS) assay). The antioxidant properties were measured by spectrophotometric methods and polyphenolic profiles were determined by HPLC-DAD for 50% ethanol extracts (10% w/v). Strong cytotoxic effect was recorded for tansy and yarrow extracts (125–1000 µg/mL) by xCELLigence system and MTS assay. Conversely, a supportive effect on cell proliferation was recorded for goldenrod extracts (125 µg/mL) by the same methods (p < 0.001). The antioxidant activity was in good correlation with total polyphenolic content, and the highest value was recorded for goldenrod leaves, followed by tansy leaves, goldenrod flowers and yarrow leaf extracts. The goldenrod extracts were abundant with flavonoids, whereas phenolic acid derivatives predominated in the polyphenolic profile of tansy and yarrow.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 99%

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Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

et al. 2020

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“…Polyphenolic compounds, classified as flavonoids (including luteolin, quercetin, chrysoeriol and their glycosides) and phenolic acids (caffeic, chlorogenic, coumaric, caftaric acid and others), have attracted a great interest of researches on their broad distribution in plants, due to high antioxidant activities [16]. The most common studied flavonoids are the 7-glycosides of apigenin and luteolin.…”

Section: D-glucopyranosyl]-β-hydroxy-γ-butyrolactone)mentioning

confidence: 99%

Antioxidant Activity of Taraxacum Officinale

Fulga¹,

Pantea²,

Andronache³

et al. 2021

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Background. The oxygen is the ultimate electron acceptor in the electron flow system that produces energy. Problems appears when the electron flow becomes uncoupled thus, generating free radicals. While the majority of processes require oxygen, the last is a highly reactive molecule that can damage cells by producing reactive oxygen species, such as hydroxyl radical, superoxide radical, hydrogen peroxide and hypochlorous acid, all together defined as triggers of oxidative stress. The last is involved in many types of pathologies, such as cancer, atherosclerosis and rheumatic arthritis. Antioxidants can stop formation of free radical by giving hydrogen atoms or scavenging them. The antioxidant can be defined any molecule capable of preventing or blocking oxidation of lipids, proteins and nucleic acids. The reduced glutathione (GSH), as well enzymes, glutathione peroxidase (GPO), glutathione reductase (GR) and glutathione S-transferase (GST) represent antioxidants which prevent the formation of new free radicals, converting them into less harmful molecules. The present study was conducted to assess the antioxidant activity of Taraxacum officinale (TO) ethanolic extracts with different bioactive contents. This experiment was conducted with extracts prepared from dandelion (Taraxacum officinale F. H. Wigg) leaves. A series (10%, 20%, 25%, 40%, 50% and 80%) of ethanolic extracts were made. The influence of TO extracts on RBC’s GPO, GR, GST, glutathione (GSSG, GSH and total-GSH) and thiols (native and total) was evaluated by measuring absorbance spectrophotometrically. Conclusions. Taraxacum officinale ethanolic extracts exhibit a strong antioxidant activity. This plant is capable of preventing damage of cells during oxidative stress process, by using different targets of glutathione system. But, this activity depends of ethanol concentrations, maybe due to different bioactive content.

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“…The antioxidative activities of herbal medicines have received a great amount of attention as being primary preventive ingredients against various diseases, such as cancer, inflammation, and cardiovascular diseases [ 13 ]. Recent studies have described that the antioxidant properties of herbal medicines are mainly due to their phenolic compounds, such as flavonoids and phenolic acids [ 14 , 15 ]. Although the preliminary studies showed that the TM extract exhibited high antioxidative activity, no research has been conducted to compare the antioxidant activities of different parts of TM, and the associations between the bioactive compounds and the antioxidative activity of TM was also unclear.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Bioactive Phenolic Compounds and Antioxidant Activities of Different Parts of Taraxacum mongolicum

et al. 2020

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Herbs derived from Taraxacum genus have been used as traditional medicines and food supplements in China for hundreds of years. Taraxacum mongolicum is a famous traditional Chinese medicine derived from Taraxacum genus for the treatment of inflammatory disorders and viral infectious diseases. In the present study, the bioactive phenolic chemical profiles and antioxidant activities of flowers, leaves, and roots of Taraxacum mongolicum were investigated. Firstly, a high performance liquid chromatography method combined with segmental monitoring strategy was employed to simultaneously determine six bioactive phenolic compounds in Taraxacum mongolicum samples. Moreover, multivariate statistical analysis, including hierarchical clustering analysis, principal component analysis, and partial least squares discriminant analysis were performed to compare and discriminate different parts of Taraxacum mongolicum based on the quantitative data. The results showed that three phenolic compounds, caftaric acid, caffeic acid, and luteolin, could be regarded as chemical markers for the differences of flowers, leaves, and roots of Taraxacum mongolicum. In parallel, total phenolic contents, total flavonoid contents and antioxidant activities of different parts of Taraxacum mongolicum were also evaluated and compared. It is clear that Taraxacum mongolicum had antioxidant properties, and the antioxidant capacities of different parts of Taraxacum mongolicum in three antioxidant assays showed a similar tendency: Flowers ≈ leaves > roots, which revealed a positive relationship with their total phenolic and flavonoid contents. Furthermore, to find the potential antioxidant components of Taraxacum mongolicum, the latent relationships of the six bioactive phenolic compounds and antioxidant activities of Taraxacum mongolicum were investigated by Pearson correlation analysis. The results indicated caftaric acid and caffeic acid could be the potential antioxidant ingredients of Taraxacum mongolicum. The present work may facilitate better understanding of differences of bioactive phenolic constituents and antioxidant activities of different parts of Taraxacum mongolicum and provide useful information for utilization of this herbal medicine.

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Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method

Cited by 36 publications

References 40 publications

Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile

Antioxidant Activity of Taraxacum Officinale

Comparison of Bioactive Phenolic Compounds and Antioxidant Activities of Different Parts of Taraxacum mongolicum

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