Polyphenols Content and In Vitro α-Glycosidase Activity of Different Italian Monofloral Honeys, and Their Effect on Selected Pathogenic and Probiotic Bacteria

Abstract

We evaluated the polyphenol content and the α-glucosidase activity exhibited by different monofloral honeys of Italian origin. Their capacity to act on different pathogenic (Acinetobacter baumannii, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus) as well as probiotic bacteria (Lacticaseibacillus casei, Lactobacillus acidophilus, Lactiplantibacillus plantarum, Lactobacillus gasseri, and Lacticaseibacillus rhamnosus) was also assessed. Total polyphenols varied between 110.46 μg/g of fresh product (rhododendron honey) and 552.29 μg/g of fresh product (strawberry tree honey). Such result did not correspond to a parallel inhibitory α-glycosidase activity that, in each case was never higher than 33 μg/mL. Honeys were differently capable to fight the biofilm formation of the pathogens (inhibition up to 93.27%); they inhibited the in vitro adhesive process (inhibition up to 84.27%), and acted on mature biofilm (with values up to 76.64%). Their effect on bacterial metabolism was different too. Honeys were ineffective to inhibit E. coli mature biofilm nor to act on its metabolism. The action of the honey on probiotic strains seemed almost always stimulate their growth. Thus, these monofloral honeys might exhibit effects on human health and act positively as prebiotics.

Benzimidazolium salts bearing the trifluoromethyl group as organofluorine compounds: Synthesis, characterization, crystal structure, in silico study, and inhibitory profiles against acetylcholinesterase and α‐glycosidase

“…[29][30][31] DM has become a chronic metabolic disorder that has become a rapidly increasing epidemic in some countries of the world due to the increase in the aging population and inadequate health services. [32][33][34] However, it is well known that carbohydrate-related enzyme inhibitors such as α-glycosidase (α-Gly) by delaying glucose absorption such as acarbose (ACR), voglibose, and miglitol are some of the most effective control methods to overcome postprandial hyperglycemia. The α-Gly inhibitors have a large drug potential.…”

Chemical Composition of Essential Oils of Bulbs and Aerial Parts of Two Cultivars of Allium sativum and Their Antibiofilm Activity against Food and Nosocomial Pathogens

“…The EOs obtained from the two cultivars ‘Bianco del Veneto’ and ‘Staravec’, could inhibit biofilm formation and, in some cases, such as against E. coli and L. monocytogenes , were capable of affecting their metabolism. However, Fratianni et al [ 48 ], analyzing the action of different types of honey on some pathogenic bacteria, observed that the inhibitory effect on biofilm formation is not always correlated with an analog effect on the bacterial metabolism within the biofilm. Similarly, the action of different seed oils against A. baumannii , E. coli , L. monocytogenes , P. aeruginosa , and S. aureus was sometimes different, depending on the microorganism [ 49 ].…”

A Sweeter Pill to Swallow: A Review of Honey Bees and Honey as a Source of Probiotic and Prebiotic Products

“…However, on the other hand, it did not influence E. Coli mature biofilm inhibition or metabolism. The LAB probiotics which comprise L. acidophilus, L. gasseri, L. casei, L. rhamnosus, and L. plantarum were shown to grow faster in honey with prebiotic potential [59]. The growth of bifidobacterial species isolated from the faeces of breastfed infants with medical-grade honey and inulin was enhanced with the non-substantial difference among both.…”

“…However, on the other hand, it did not influence E. Coli mature biofilm inhibition or metabolism. The LAB probiotics which comprise L. acidophilus , L. gasseri , L. casei , L. rhamnosus , and L. plantarum were shown to grow faster in honey with prebiotic potential [ 59 ].…”