Chemical Composition of Essential Oils of Bulbs and Aerial Parts of Two Cultivars of Allium sativum and Their Antibiofilm Activity against Food and Nosocomial Pathogens

Abstract

This work aimed to evaluate the chemical composition of the essential oils (EOs) of two cultivars of Allium sativum and their antibiofilm activity against the food pathogens Acinetobacter baumannii, Escherichia coli, Listeria monocytogenes, and Staphylococcus aureus. The crystal violet assay ascertained the susceptibility of the bacterial biofilms, while the MTT assay let to evaluations of the metabolic changes occurring in the bacterial cells within biofilms. Their chemical composition indicated some sulfuric compounds (i.e., allicin, diallyl disulfide, and allyl propyl disulfide), and decene as some of the main components of the EOs. The aerial parts and bulbs’ EOs from the two cultivars showed chemical differences, which seemed to affect the antibiofilm activity. The EOs from aerial parts of ‘Bianco del Veneto’ inhibited the biofilm formation of L. monocytogenes and E. coli (60.55% and 40.33%, respectively). In comparison, the ‘Staravec’ EO inhibited the cellular metabolism of E. coli (62.44%) and S. aureus (51.52%) sessile cells. These results indicate their possible use as preserving agents in the food industry and suggest their potential exploitation in the development of new formulations to avoid or limit nosocomial infections.

Chemical Composition, Phytotoxic and Antibiofilm Activity of Seven Eucalyptus Species from Tunisia

“…The plates were then closed with parafilm to prevent dehydration. The wide-spectrum conventional antibiotic tetracycline, previously suspended in DMSO, and used in other similar research [ 41 , 42 ] was added in a column of the plate as positive control. Luria–Bertani broth containing resazurin and bacteria, without samples, was considered as the negative control.…”

Natural Strategies as Potential Weapons against Bacterial Biofilms

“…The EOs from the aerial parts of Staravec exhibited effective inhibition more effectively against Acinetobacter baumannii (45.61%), while EOs from the bulbs of Staravec showed no inhibition. The outcomes revealed their potential application as potential antibiofilm agents in the food industry and health sector as well [ 166 ].…”

Antimicrobial, Herbicidal and pesticidal potential of Tunisian eucalyptus species: Chemoprofiling and biological evaluation

“…Biofilm inhibitory activity was determined as a percentage relative to the control. Triplicate experiments were performed for reliability [ 27 ].…”

“…The metabolic activity of bacterial cells within the EOs-influenced biofilm was assessed utilizing the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) colorimetric method [ 27 ].…”