Polyphenolic metabolites of Epilobium hirsutum

Dethe

et al. 2015

Pharmaceutical Biology

Context: Syzygium cumini (L.) Skeels (Myrtaceae), commonly known as jamun, is an Indian plant, traditionally well known for its medicinal properties including antidiabetic activity. Objective: To isolate the antidiabetic compounds from Syzygium cumini seeds and evaluate their activity using aldose reductase (AR) and protein-tyrosine phosphatase 1B (PTP1B) inhibition assays. Materials and methods:The dried seeds were extracted with methanol and partitioned with ethyl acetate, butanol, and water. The extracts were screened for antidiabetic activity at a concentration of 100 mg/mL using in vitro AR and PTP 1B inhibition assays. Results and discussion: The highly enriched fractions obtained from broad ethyl acetate fraction yielded maslinic acid (1), 5-(hydroxymethyl) furfural (2), gallic acid (3), valoneic acid dilactone (4), rubuphenol (5), and ellagic acid (6). Structures were elucidated by 1 H-NMR and 13 C-NMR. The initial ethyl acetate fraction showed AR inhibitory activity with the IC 50 value of 2.50 mg/mL and PTP1B enzyme inhibition with the IC 50 value of 26.36 mg/mL. Compounds 3, 4, 5, and 6 were found to inhibit AR with IC 50 values of 0.77, 0.075, 0.165, and 0.12 mg/mL while the compounds 4, 5, and 6 inhibited PTP1B with IC 50 values of 9.37, 28.14, and 25.96 mg/mL, respectively. Conclusion:The results of this study demonstrate that the isolated constituents show promising in vitro antidiabetic activity and, therefore, can be candidates for in vivo biological screening using relevant models to ascertain their antidiabetic activity.

Section: Resultsmentioning

confidence: 54%

Aldose reductase and protein tyrosine phosphatase 1B inhibitory active compounds fromSyzygium cuminiseeds

Sawant

Dethe

et al. 2015

Pharmaceutical Biology

“…So TLC method was also reported for adulteration analysis of B. diffusa with Trienthema portulacastrum Linn. in different Ayurvedic formulations, so it employed as an effective tool for quality control of market drugs 31,32 . Similarly HPLC, TLC, AAS and HNMR were used in fractions and extract of T. portulacastrum and B. diffusa to determine important bioactive compounds 34 and it has been found chromatographic methods are simple, rapid and inexpensive in identification and comparison of Boeravinone 38 .…”

Section: Introductionmentioning

confidence: 99%

Identification and Quantification of Boeravinone-B in dry fruit extract of Boerhaavia diffusa Linn and in its Polyherbal Formulation

Sharma

Journal of Natural Remedies

et al. 2017

Objective: To ascertain the authenticity of Boeravinone-B in Boerhaavia diffusa and its poly-herbal dosage form (capsule) by identification and quantification through precise and robust HPLC method. Materials and Methods: The RP-HPLC analysis was accomplished employing phosphate buffer and acetonitrile as mobile phase at a flow rate of 1.5 ml/min and 270 nm as detection wavelength. Results: The content of Boeravinone B in ingredients (hydro-alcoholic extracts of whole plant) and poly-herbal formulation of B. diffusa was estimated as 0.041% w/w and 0.011% w/w, respectively with good linearity R2>0.9997 and retention time 24.413±0.004 min. Conclusion: The amount of Boeravinone-B present in B.diffusa extracts of raw drug complies with that of reported in literature. Quantification of Boeravinone-B suggested an identification mark for this drug and its different market formulations and may work as quality parameter in standardization. On the basis of percentage of Boeravinone-B the content of B.diffusa in poly-herbal formulation validate the ratio of ingredient in final formulation. This method proved its applicability in quality assessment via quantification of this marker in raw material as well as various phyto-formulations of B. diffusa and widens the acceptability criteria at international level. Identification and Quantification of Journal of natural remedies AbstractIpomoea reniformis Chaos is claimed in Indian traditional medical practice to be useful in the treatment of epilepsy and neurological disorders. In the present study, pretreatment effect of methanolic extract of Ipomoea reniformis on epilepsy and psychosis was evaluated in rodents using standard procedures. Besides evaluating epileptic and behavioral parameters, neurotransmitters such as Gamma-Amino Butyric Acid (GABA) in epilepsy and in psychosis dopamine, noradrenaline and serotonin contents in the rodent brain were estimated. The extract pretreatment reduced maximal electro shock; Isoniazid (INH) and Pentylenetetrazole (PTZ) induced seizures and also significantly inhibited the attenuation of brain GABA levels by INH and PTZ in mice. These results suggested that the observed beneficial effect in epilepsy may be by enhancing the GABAergic system. The test drug also inhibited the apomorphine induced climbing and stereotyped behavior and showed significantly reduced levels of brain dopamine, noradrenaline and serotonin which may be due to blocking of central dopaminergic, noradrenergic and serotonergic pathways or by enhancing the GABAergic system. The results obtained in present study suggest that the title plant possesses antiepileptic and antipsychotic activities in rodents.

“…Furthermore, Epilobium species are described as nontoxic [6]. Epilobium species' active phytocompounds are polyphenols such as ellagitannins, flavonoids (e.g., anthocianidins and miricetin and quercetin derivates) and gallic acid derivatives along with saponins, volatile oils and vitamins [12][13][14], essential amino acids (e.g., tyrosine, serine, threonine, cysteine, valine, serine, leucine and isoleucine, phenylalanine, proline) [15] and minerals (e.g., K, Mg. P, S, Cu, Co, Zn) [16].…”

Section: Introductionmentioning

confidence: 99%

Epilobi Hirsuti Herba Extracts Influence the In Vitro Activity of Common Antibiotics on Standard Bacteria

et al. 2016

Epilobium genus has been confirmed as an effective source of natural antimicrobials. However, the influence of Epilobi hirsuti herba derived products on usual antibiotics activity has not been studied. In this study, several standardized Epilobi hirsuti herba extracts (EHE) were evaluated in order to asses their potential effects on usual antibiotics tested on standard Grampositive and Gram-negative bacterial strains in vitro. The results emphasized that the bacterial strains ranged from sensitive (MIC values between 50-200 μg GAE mL -1 ) (S. epidermidis ATCC 12228) to very resistant (E. coli strains), E. faecalis ATCC 29212 being practically immune to EHE. In terms of synergistic interaction, Tetracycline and Ampicillin combinations lead to the most important stimulatory effects, the diameters of the inhibition zone being even 60% bigger compared to the antibiotic alone. Synergistic effects between myricetin(galloyl) derivates and Tetracycline were also revealed on P. aeruginosa and E. coli strains. Together, it clearly demonstrated not only EHE's own antimicrobial properties, but also their capacity to influence the antimicrobial potency of some common antibiotics. These results could be useful for the area of herbal medicines and as potential candidates in managing microbial resistance, but also for physicians and pharmacists using combined antibacterial therapy.