Background:
Relations among markers, quality assessment and standardization of
classical preparations like Triphala an Ayurvedic potent formulation are necessary for the selectivity
as well as acceptability of genuine plant drugs and formulation.
Objective:
Qualitative and quantitative evaluations of three batches of in-house Triphala
along with its ingredients collected from three different locations of India with respect to
assess the six active markers.
Method:
Phytochemical studies, spectrophotometric estimations (TPC & TFC), chromatographic
(HPLC & HPTLC) methods were developed for the identification and quantification of
active markers in Triphala.
Result:
Chemical analysis and HPTLC profiles with respect of gallic acid at Rf 0.35 of
methanol extracts showed the presence of almost similar phytochemicals in three batches.
The highest HPLC peak % area for corrilagin, 1,3,6-Trigalloyl-beta-D-glucose, ellagic acid
and chebulinic acid was calculated to be 3.753, 5.27, 24.55 and 29.47, respectively with a majority
of markers i.e. four observed in batch-III. The percentage amount of TPC at λ max 720
for batch-III of Phyllanthus emblica L., Terminalia bellirica Roxb., Terminalia chebula Retz.
and Triphala was 393.1, 374.81, 628 & 644.5 mg of TAE/g dry weight equivalent, respectively.
Similarly, TFC at λ max 510 for the same batch and ingredients was calculated to be
60.27, 40.043, 74.84 and 59.21 QUE/g dry weight equivalent, which were also observed to be
maximum in batch-III.
Conclusion:
Batch-III of Triphala is of the highest quality and up to pharmacopoeial standards
(API). It may be used to predict the quality and efficacy of various commercial formulations
of Triphala. These outcomes may be utilized in pharmaceuticals for routine batch standardization
and quality control.
Objective: To ascertain the authenticity of Boeravinone-B in Boerhaavia diffusa and its poly-herbal dosage form (capsule) by identification and quantification through precise and robust HPLC method. Materials and Methods: The RP-HPLC analysis was accomplished employing phosphate buffer and acetonitrile as mobile phase at a flow rate of 1.5 ml/min and 270 nm as detection wavelength. Results: The content of Boeravinone B in ingredients (hydro-alcoholic extracts of whole plant) and poly-herbal formulation of B. diffusa was estimated as 0.041% w/w and 0.011% w/w, respectively with good linearity R2>0.9997 and retention time 24.413±0.004 min. Conclusion: The amount of Boeravinone-B present in B.diffusa extracts of raw drug complies with that of reported in literature. Quantification of Boeravinone-B suggested an identification mark for this drug and its different market formulations and may work as quality parameter in standardization. On the basis of percentage of Boeravinone-B the content of B.diffusa in poly-herbal formulation validate the ratio of ingredient in final formulation. This method proved its applicability in quality assessment via quantification of this marker in raw material as well as various phyto-formulations of B. diffusa and widens the acceptability criteria at international level.
Identification and Quantification of
Journal of natural remedies
AbstractIpomoea reniformis Chaos is claimed in Indian traditional medical practice to be useful in the treatment of epilepsy and neurological disorders. In the present study, pretreatment effect of methanolic extract of Ipomoea reniformis on epilepsy and psychosis was evaluated in rodents using standard procedures. Besides evaluating epileptic and behavioral parameters, neurotransmitters such as Gamma-Amino Butyric Acid (GABA) in epilepsy and in psychosis dopamine, noradrenaline and serotonin contents in the rodent brain were estimated. The extract pretreatment reduced maximal electro shock; Isoniazid (INH) and Pentylenetetrazole (PTZ) induced seizures and also significantly inhibited the attenuation of brain GABA levels by INH and PTZ in mice. These results suggested that the observed beneficial effect in epilepsy may be by enhancing the GABAergic system. The test drug also inhibited the apomorphine induced climbing and stereotyped behavior and showed significantly reduced levels of brain dopamine, noradrenaline and serotonin which may be due to blocking of central dopaminergic, noradrenergic and serotonergic pathways or by enhancing the GABAergic system. The results obtained in present study suggest that the title plant possesses antiepileptic and antipsychotic activities in rodents.
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