Polyphenolic Compounds and Antioxidant Activity of Cold‐Pressed Seed Oil from Finola Cultivar of Cannabis sativa L.

Abstract

The aim of this study was to characterize the polyphenolic compounds and antioxidant activity of cold-pressed seed oil from Finola cultivar of industrial hemp (Cannabis sativa L.). Several methodologies have been employed to evaluate the in vitro antioxidant activity of Finola hempseed oil (FHSO) and both lipophilic (LF) and hydrophilic fractions (HF). The qualitative and quantitative composition of the phenolic fraction of FHSO was performed by HPLC analyses. From the results is evident that FHSO has high antioxidative activity, as measured by DPPH radical (146.76 mmol of TE/100 g oil), inhibited β-carotene bleaching, quenched a chemically generated peroxyl radical in vitro and showed high ferrous ion chelating activity. Reactivity towards 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical cation and ferric-reducing antioxidant power values were 695.2 µmol of TE/100g oil and 3690.6 µmol of TE/100 g oil respectively. FHSO contains a significant amount of phenolic compounds of which 2780.4 mg of quercetin equivalent/100 g of total flavonoids. The whole oil showed higher antioxidant activity compared with LF and HF. Our findings indicate that the significant antioxidant properties shown from Finola seed oil might generally depend on the phenolic compounds, especially flavonoids, such as flavanones, flavonols, flavanols and isoflavones. Copyright © 2016 John Wiley & Sons, Ltd.

In Vitro Evaluation of the Antioxidant, Cytoprotective, and Antimicrobial Properties of Essential Oil from Pistacia vera L. Variety Bronte Hull

“…The total phenols content was determined according to Smeriglio et al [37] using gallic acid as the reference compound and the results were expressed as mg of gallic acid equivalents (GAE)/100 g of essential oil.…”

“…The antioxidant activity against ABTS •+ radical was carried out according to Smeriglio et al [37]. Briefly, the reaction mixture (4.3 mM potassium persulfate and 1.7 mM ABTS solution 1:5, v / v ) was incubated for 12–16 h in the dark at room temperature, and was diluted, before use, with phosphate buffer (pH 7.4) in order to obtain an absorbance at 734 nm of 0.7 ± 0.02.…”

“…The free-radical scavenging capacity against TPTZ radical was performed according to Smeriglio et al [37]. Twenty-five microliters (25 µL) of sample solution (range 150–25 µg/mL) was added to 1.5 mL of daily fresh FRAP reagent pre-warmed at 37 °C, and the absorbance was recorded at 593 nm by an UV-VIS Spectrophotometer (Shimadzu UV-1601) after an incubation time of 4 min at 20 °C, with the FRAP reagent used as blank.…”

“…Fe 2+ chelating capacity was evaluated as described by Smeriglio et al [37] with some modifications. Briefly, 25 µL of FeCl 2 ∙4H 2 O solution (1.8 mM) was added to 50 µL of sample solution (range 60–7.5 µg/mL) and incubated at RT for 5 min.…”

“…The β-carotene bleaching assay was performed using an emulsion prepared according to Smeriglio et al [37]. Aliquots of this emulsion (7.0 mL) were mixed with 0.28 mL of sample solution (range 4–0.5 mg/mL).…”