Polyphasic characterization of Brazilian Rhizobium tropici strains effective in fixing N2 with common bean (Phaseolus vulgaris L.)

Pinto, Fabiana Gisele da Silva; Hungría, Mariangela; Mercante, F. M.

doi:10.1016/j.soilbio.2007.01.001

Cited by 56 publications

(48 citation statements)

References 64 publications

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“…Similarly, Pinto et al (2007) showed that the majority of very effective rhizobia isolated from common bean fi eld-grown plants were R. tropici.…”

Section: Resultsmentioning

confidence: 98%

“…This suggests a possible environmental instability of these strains in Brazilian soil conditions, since one of them (LBMP-4VE), although identifi ed as R. tropici, did not show a positive response in the acid soil evaluated (pH 5.9). This was unexpected, because Mostasso et al (2002) emphasized that R. tropici is a good choice for acid soils with high temperatures, and Pinto et al (2007) found that this species exhibited strong competitiveness, tolerance of stressful environmental conditions and genetic stability.…”

Section: Af3648391mentioning

confidence: 99%

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Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Gonzalez

Campanharo

Lemos

2008

Pesq. agropec. bras.

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-This study aimed to genetically characterize four new Rhizobium strains, and to evaluate their nodulation and fi xation capacity compared to commercial strains and to native rhizobia population of a Brazilian Rhodic Hapludox. Two experiments were carried out in randomized blocks design, under greenhouse conditions, in 2007. In the fi rst experiment, the nodulation and nitrogen fi xation capacity of new strains were evaluated, in comparison to the commercial strains CIAT-899 and PRF-81 and to native soil population. It was carried out in plastic tubes fi lled with vermiculite. DNA extractions and PCR sequencing of the intergenic space were made from the isolated pure colonies, in order to genetically characterize the strains and the native rhizobia population. In the second experiment, the nodulation and productivity of common beans Perola cultivar were determined, with the use of evaluated strains, alone or in mixture with PRF-81 strain. It was carried out in pots fi lled with soil. The native soil population was identifi ed as Rhizobium sp. and was ineffi cient in nitrogen fi xation. Three different Rhizobium species were found among the four new strains. The LBMP-4BR and LBMP-12BR new strains are among the ones with greatest nodulation and fi xation capacity and exhibit differential responses when mixed to PRF-81.Index terms: Phaseolus vulgaris, biological nitrogen fi xation, intergenic space, phylogeny, sequencing, soil rhizobia population. Caracterização genética e capacidade de fi xação de nitrogênio de estirpes de Rhizobium em feijoeiroResumo -O objetivo deste trabalho foi a caracterização genética de quatro novas estirpes de Rhizobium e a avaliação de sua capacidade de fi xação de N 2 e nodulação, comparadas a estirpes comerciais e à população nativa de rizóbios de um Latossolo Vermelho. Dois experimentos foram conduzidos em blocos ao acaso, em casa de vegetação. No primeiro experimento, conduzido em tubetes com vermiculita, avaliaram-se a nodulação e a capacidade de fi xação das novas estirpes, em comparação com as estirpes comerciais CIAT-899 e PRF-81 e com a população nativa do solo. Das colônias puras isoladas, extraiu-se o DNA genômico e realizou-se o seqüenciamento do espaço intergênico, para a caracterização genética das estirpes e da população nativa de rizóbios. O segundo experimento foi realizado em vasos com solo, para determinação da produtividade e da nodulação do feijoeiro, cultivar Pérola, com o uso das estirpes isoladamente ou em mistura com a PRF-81. A população nativa do solo foi identifi cada como Rhizobium sp. e se mostrou inefi ciente na fi xação de nitrogênio. Foram encontradas três espécies de Rhizobium entre as quatro novas estirpes. As estirpes LBMP-4BR e LBMP-12BR estão entre as que têm maior capacidade de nodulação e fi xação de N 2 , e apresentam respostas diferenciadas quando misturadas à PRF-81.Termos para indexação: Phaseolus vulgaris, fi xação biológica de nitrogênio, espaço intergênico, fi logenia, seqüenciamento, população nativa de rizóbios.

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“…Similarly, Pinto et al (2007) showed that the majority of very effective rhizobia isolated from common bean fi eld-grown plants were R. tropici.…”

Section: Resultsmentioning

confidence: 98%

Section: Af3648391mentioning

confidence: 99%

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Gonzalez

Campanharo

Lemos

2008

Pesq. agropec. bras.

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“…In recent years the classification of these microorganisms had been carried out with polyphasic taxonomic approaches, which aggregates phenotypic, genotypic and phylogenetic information (14,17,30,33,34). The studies that involve phenotypic and genetic characterization of rhizobial strains are important to elucidate relationships among these strains.…”

mentioning

confidence: 99%

Characterization of indigenous rhizobia from Caatinga

Teixeira

Borges²,

Xavier³

et al. 2010

Braz. J. Microbiol.

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The aim of this study was to characterize rhizobial isolates from Cratylia mollis Mart. ex Benth, Calliandra depauperata Benth. and Mimosa tenuiflora (Willd.) Poir. by means of rhizobial colonies morphology and restriction analysis of the 16S ribosomal gene (16S rDNA-ARDRA). Nodules were collected in the field and from plants cultivated in a greenhouse experiment using Caatinga soil samples. Sixty seven isolates were described by morphological analysis. Forty seven representative isolates were used for ARDRA analysis using seven restriction enzymes. We observed high diversity of both slow and fast-growing rhizobia that formed three morpho-physiological clusters. A few fast-growing isolates formed a group of strains of the Bradyrhizobium type; however, most of them diverged from the B. japonicum and B. elkanii species. Cratylia mollis nodule isolates were the most diverse, while all Mimosa tenuiflora isolates displayed fast growth with no pH change and were clustered into groups bearing 100% similarity, according to ARDRA results.

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“…1999). This indicates that most of isolates obtained in this study, regardless of soil origin, have characteristics similar to those of Rhizobium genus, due mainly to the acidity of the culture medium and displaying fast growth, as well as other features like colonies with a diameter >2 mm and high mucus production (PINTO et al, 2007;MARQUINA et al 2011;DALL'AGNOL et al 2013). Nevertheless, high morphological diversity was observed among the obtained isolates, whereas the isolates showed unusual characteristics for the genus Rhizobium, such as neutral pH, slow growth and small production of mucus (Figure 1).…”

Section: Morphological Characterization Of Rhizobia Isolatesmentioning

confidence: 59%

“…All those characteristics are common among bacteria belonging to the genus Rhizobium (PINTO et al, 2007;MARQUINA et al 2011;DALL'AGNOL et al 2013). Among the isolates obtained from soil samples from Goiás, most of those acidified the culture medium, formed colonies >2 mm, showed fast growth, yellow coloring, few quantity of mucus and heterogeneous appearance ( Figure 1A).…”

Section: Morphological Characterization Of Rhizobia Isolatesmentioning

confidence: 98%

Morphophysiological characterization of rhizobia isolated from wild genotypes of the common bean

Sampaio¹,

Knupp²,

Fernandes³

et al. 2016

Biosci. J.

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ABSTRACT:This study aimed to characterize, based on the morphology and on the use of carbon sources, rhizobial isolates obtained from nodules of wild common bean genotypes cultivated on soil samples from the states of Goiás, Minas Gerais and Paraná. Based on morphological and carbon source use, similarity matrixes were generated to perform clustering analysis based on the Jaccard index. A total of 523 isolates were obtained with the predominance of isolates that acidify the culture medium and showed fast growth. Isolates from the soils of Araucária and Prudentópolis produced a greater amount of mucus than those from Jussara, Nova Veneza, Uberlândia and Unaí soils. The rhizobia isolates obtained from soils of Goiás and Minas Gerais showed greater phenotypic diversity than those from the soils of Paraná. Analysis based on the use of different carbon sources revealed that rhizobia isolates obtained from soils of Goiás have higher metabolic diversity. Overall, the most part of isolates grouped with the reference strains, especially with the Rhizobium tropici and R. freirei strains used as inoculants for the common bean.

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Polyphasic characterization of Brazilian Rhizobium tropici strains effective in fixing N2 with common bean (Phaseolus vulgaris L.)

Cited by 56 publications

References 64 publications

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Genetic characterization and nitrogen fixation capacity of Rhizobium strains on common bean

Characterization of indigenous rhizobia from Caatinga

Morphophysiological characterization of rhizobia isolated from wild genotypes of the common bean

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