Polyols Induce the Production of Antifungal Compounds by Lactobacillus plantarum

Espinosa-Salgado, Rubén; Tamayo-Galván, Victoria; Perraud-Gaime, Isabelle; Rodríguez-Serrano, Gabriela; González-Robles, Rosa Obdulia; Durand, Noël; Champion-Martínez, Elena Isabel; Saucedo‐Castañeda, Gerardo

doi:10.1007/s00284-022-02761-4

Cited by 7 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These groups of compounds have been evaluated for their antimicrobial, insecticidal, vasodilator, anti-tuberculosis, antioxidant, anti-viral, anti-prion, antihyperglycemic, and cytotoxic activities to demonstrate their potential applications in food, medicine, and agriculture [62]. Cyclo (Val-Phe), for instance, contributes to the antifungal properties of Lactobacillus plantarum [63], Cyclo (Ile-Tyr) of Pseudomonas rhizosphaerae exhibits antibacterial and antilarval potentials [64], and Cyclo (Phe-Pro) is claimed to induce apoptosis and thus to be effective as an antitumor molecule [65][66][67]. To the best of our knowledge, Cyclo (Val-Phe), Cyclo (Ile-Tyr), and Cyclo (Phe-Pro) are identified for the first time in this species.…”

Section: Discussionmentioning

confidence: 99%

Structural Diversity, LC-MS-MS Analysis and Potential Biological Activities of Brevibacillus laterosporus Extract

et al. 2022

View full text Add to dashboard Cite

Lake Mariout is Egypt’s degraded coastal marine habitat that encompasses a variety of wastes. The biodiversity and hard environmental conditions allow the co-existence of organisms with high resistance and rich metabolism, making them potential candidates for screening and isolating novel microbial strains. A bacterial isolate (BF202) cultured from the marine sediments of Alexandria’s Mariout Lake (Egypt) was tested for its antimicrobial and anticancer potential. The phylogenetic analysis of the isolated strain’s 16S rDNA and gyrB revealed that BF202 belongs to Brevibacillus laterosporus (B. laterosporus). Antibiosis of B. laterosporus was confirmed against microbial pathogens including Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, and Staphylococcus aureus. The highest antibacterial activity was detected on glucose peptone medium after 18 h of incubation at 35 °C, and at pH of 7.0 in the presence of mannose and ammonium carbonate as carbon and nitrogen sources, respectively. The cytotoxicity of the methanolic extract against breast cancer (MCF-7) and normal Vero cell lines, using the MTT test, revealed IC50 values of 7.93 and 23.79 µg/mL, respectively. To identify apoptotic and necrotic cells, a flow cytometric analysis using annexin V-FITC/PI dual-labeling was utilized and recorded a higher number of necrotic cells compared to apoptotic ones. Similarly, the cell cycle S-phase arrest was reported. The LC-MS-MS investigation of B. laterosporus extract and the molecular networking database analysis demonstrated five strategic diketopiperazine compounds with antimicrobial and anticancer activities. Taken together, this research shows that the crude extract of B. laterosporus might be an effective agent against drug-resistant bacteria and malignant disorders due to its richness in diketopiperazines.

show abstract

Section: Discussionmentioning

confidence: 99%