Polymyxin resistance in Enterobacterales: overview and epidemiology in the Americas

Rodríguez-Santiago, Jonathan; Cornejo‐Juárez, Patricia; Silva-Sánchez, Jesús; Garza–Ramos, Ulises

doi:10.1016/j.ijantimicag.2021.106426

Cited by 29 publications

(33 citation statements)

References 284 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, the self-transmissible 170-kb IncHI2 plasmid had a major distribution in the mcr-1 positive isolates (Table 1). This is consistent with the fact that the spread of the mcr-1 gene in Latin America seems to be more related to the spread of mcr-1 carrying plasmids among E. coli isolates than to the clonal expansion of MCR-1 producing isolates [6]. The most prevalent mcr-1 carrying plasmids in Latin America are the IncX4-and IncI2-types.…”

Section: Discussionsupporting

confidence: 85%

“…These plasmids are transferable with a broad host range, which could explain the widespread of mcr genes among different bacterial species [ 4 ]. Ten variants of the mcr gene have so far been described [ 5 ], but the most widespread is the mcr-1 gene [ 6 ]. This gene has been identified in isolates from numerous sources including humans, food, farms and wild animals [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…It has been demonstrated that the transfer of the mcr-1 gene in enterobacteria from farm animals to humans through the food chain [ 9 ]. Notably, mcr genes are found in association with other antimicrobial resistance genes, usually extended-spectrum-ß-lactamases (ESBL) and carbapenemases, highlighting the emergence of pan-drug resistant Enterobacterales [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…In Mexico, polymyxins, as well as other antibiotics, have not been approved as growth promoters since 2012, and there are few reports of isolates carrying the mcr-1 gene [ 6 ]; nevertheless, this gene has been identified from clinical [ 13 ] and swine farm [ 14 ] environments. In addition, it has also been described in cantaloupes [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular and Genomic Insights of mcr-1-Producing Escherichia coli Isolates from Piglets

et al. 2022

Self Cite

View full text Add to dashboard Cite

The use of colistin in food-producing animals favors the emergence and spread of colistin-resistant strains. Here, we investigated the occurrence and molecular mechanisms of colistin resistance among E. coli isolates from a Mexican piglet farm. A collection of 175 cephalosporin-resistant colonies from swine fecal samples were recovered. The colistin resistance phenotype was identified by rapid polymyxin test and the mcr-type genes were screened by PCR. We assessed the colistin-resistant strains by antimicrobial susceptibility test, pulse-field gel electrophoresis, plasmid profile, and mating experiments. Whole-Genome Sequencing data was used to explore the resistome, virulome, and mobilome of colistin-resistant strains. A total of four colistin-resistant E. coli were identified from the cefotaxime-resistant colonies. All harbored the plasmid-borne mcr-1 gene, which was located on conjugative 170-kb IncHI-2 plasmid co-carrying ESBLs genes. Thus, high antimicrobial resistance rates were observed for several antibiotic families. In the RC2-007 strain, the mcr-1 gene was located as part of a prophage carried on non-conjugative 100-kb-plasmid, which upon being transformed into K. variicola strain increased the polymyxin resistance 2-fold. The genomic analysis showed a broad resistome and virulome. Our findings suggest that colistin resistance followed independent acquisition pathways as clonal and non-genetically related mcr-1-harboring strains were identified. These E. coli isolates represent a reservoir of antibiotic resistance and virulence genes in animals for human consumption which could be potentially propagated into other interfaces.

show abstract

Section: Discussionsupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular and Genomic Insights of mcr-1-Producing Escherichia coli Isolates from Piglets

et al. 2022

Self Cite

View full text Add to dashboard Cite

show abstract

“…Their ability to bind the lipid A component of LPS makes them precious, the last resource weapons to fight septic shock, notwithstanding their known nephrotoxicity. Resistance has emerged, however, and is spreading at an alarming pace, putting the effectiveness of these valuable therapeutics at risk [ 170 , 171 ]. Last but not least, daptomycin.…”

Section: Amps—goods Vs Bads and The Long Way Towards Clinical Applicationmentioning

confidence: 99%

Multitalented Synthetic Antimicrobial Peptides and Their Antibacterial, Antifungal and Antiviral Mechanisms

Vanzolini

Bruschi

Rinaldi

et al. 2022

IJMS

View full text Add to dashboard Cite

Despite the great strides in healthcare during the last century, some challenges still remained unanswered. The development of multi-drug resistant bacteria, the alarming growth of fungal infections, the emerging/re-emerging of viral diseases are yet a worldwide threat. Since the discovery of natural antimicrobial peptides able to broadly hit several pathogens, peptide-based therapeutics have been under the lenses of the researchers. This review aims to focus on synthetic peptides and elucidate their multifaceted mechanisms of action as antiviral, antibacterial and antifungal agents. Antimicrobial peptides generally affect highly preserved structures, e.g., the phospholipid membrane via pore formation or other constitutive targets like peptidoglycans in Gram-negative and Gram-positive bacteria, and glucan in the fungal cell wall. Additionally, some peptides are particularly active on biofilm destabilizing the microbial communities. They can also act intracellularly, e.g., on protein biosynthesis or DNA replication. Their intracellular properties are extended upon viral infection since peptides can influence several steps along the virus life cycle starting from viral receptor-cell interaction to the budding. Besides their mode of action, improvements in manufacturing to increase their half-life and performances are also taken into consideration together with advantages and impairments in the clinical usage. Thus far, the progress of new synthetic peptide-based approaches is making them a promising tool to counteract emerging infections.

show abstract

The efficacy of anti‐proteolytic peptide R7I in intestinal inflammation, function, microbiota, and metabolites by multi‐omics analysis in murine bacterial enteritis

Sun

Liu

et al. 2022

Bioengineering & Transla Med

View full text Add to dashboard Cite

Increased antibiotic resistance poses a major limitation in tackling inflammatory bowel disease and presents a large challenge for global health care. Antimicrobial peptides (AMPs) are a potential class of antimicrobial agents. Here, we have designed the potential oral route for antimicrobial peptide R7I with anti-proteolytic properties to deal with bacterial enteritis in mice. The results revealed that R7I protected the liver and gut from damage caused by inflammation. RNA-Seq analysis indicated that R7I promoted digestion and absorption in the small intestine by upregulating transmembrane transporter activity, lipid and small molecule metabolic processes and other pathways, in addition to upregulating hepatic steroid biosynthesis and fatty acid degradation. For the gut microbiota, Clostridia were significantly reduced in the R7I-treated group, and Odoribacteraceae, an efficient isoalloLCA-synthesizing strain, was the main dominant strain, protecting the gut from potential pathogens. In addition, we further discovered that R7I reduced the accumulation of negative organic acid metabolites. Overall, R7I exerted better therapeutic and immunomodulatory potential in the bacterial enteritis model, greatly reduced the risk of disease onset, and provided a reference for the in vivo application of antimicrobial peptides.

show abstract

Polymyxin resistance in Enterobacterales: overview and epidemiology in the Americas

Cited by 29 publications

References 284 publications

Molecular and Genomic Insights of mcr-1-Producing Escherichia coli Isolates from Piglets

Molecular and Genomic Insights of mcr-1-Producing Escherichia coli Isolates from Piglets

Multitalented Synthetic Antimicrobial Peptides and Their Antibacterial, Antifungal and Antiviral Mechanisms

The efficacy of anti‐proteolytic peptide R7I in intestinal inflammation, function, microbiota, and metabolites by multi‐omics analysis in murine bacterial enteritis

Contact Info

Product

Resources

About