Polymyxin E–Resistant Gram-Negative Bacteria in Tunisia and Neighboring Countries: Are There Commonalities?

Dziri, Olfa; Dziri, Raoudha; Salabi, Allaaeddin Ali El; Alawami, Alhussain A; Ksouri, Riadh; Chouchani, Chedly

doi:10.2147/idr.s327718

Cited by 2 publications

(2 citation statements)

References 57 publications

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“…As far as North Africa is concerned, previous studies detected the highest prevalence of colistin resistance in Tunisia [ 7 ] where the presence of colistin-resistant Gram-negative bacteria has been well documented in clinical specimens [ 24 ], animals such as chickens [ 25 ], bovids [ 26 ], camels [ 27 ] and wild boars [ 28 ], retail meat [ 16 ], and wastewater [ 29 ]. Only the mcr -1 variant has been highlighted so far.…”

Section: Discussionmentioning

confidence: 99%

“…The widespread and excessive use of colistin has led to the emergence and spread of colistin resistance among Gram-negative bacteria. The livestock industry has been pointed out as the main source responsible for this phenomenon, considering the limited use of colistin in human medicine for two decades vs its extensive use in livestock production for the treatment of enterobacterial digestive infections as well as for prophylactic treatment and as a growth promoter [ 6 , 7 ]. Polymyxins, including colistin, are positively charged polypeptides that interact with the negatively charged phosphate group of lipid A of the lipopolysaccharide (LPS) of the outer membrane of Gram-negative bacteria, destabilizing the membrane to cause cell lysis [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Colistin Resistance Genes in Broiler Chickens in Tunisia

Francesco

Salvatore

Sakhria

et al. 2023

Animals

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Colistin is a polymyxin antibiotic that has been used in veterinary medicine for decades, as a treatment for enterobacterial digestive infections as well as a prophylactic treatment and growth promoter in livestock animals, leading to the emergence and spread of colistin-resistant Gram-negative bacteria and to a great public health concern, considering that colistin is one of the last-resort antibiotics against multidrug-resistant deadly infections in clinical practice. Previous studies performed on livestock animals in Tunisia using culture-dependent methods highlighted the presence of colistin-resistant Gram-negative bacteria. In the present survey, DNA extracted from cloacal swabs from 195 broiler chickens from six farms in Tunisia was tested via molecular methods for the ten mobilized colistin resistance (mcr) genes known so far. Of the 195 animals tested, 81 (41.5%) were mcr-1 positive. All the farms tested were positive, with a prevalence ranging from 13% to 93%. These results confirm the spread of colistin resistance in livestock animals in Tunisia and suggest that the investigation of antibiotic resistance genes by culture-independent methods could be a useful means of conducting epidemiological studies on the spread of antimicrobial resistance.

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