Polymorphism of transferrin in carp (Cyprinus carpio L.): Genetic determination, isolation, and partial characterization

Abstract

Seven transferrin variants (A,B,C,D,E,F, and G) have been found in carp sera (Cyprinus carpio L.). Genetic analysis involves five variants and agrees with the hypothesis of simple codominant autosomal inheritance at one transferrin (Tf) locus in spite of the fact that the carp is a tetraploid in relation to other species of the same family. Carp populations from three regions were studied which differed in gene frequencies. Individual populations were in Hardy-Weinberg equilibrium. The polymorphism of carp transferrins can be used for the identification of offspring of single parent pairs, stocked in one pond. Transferrins have been isolated and characterized. Homozygous phenotypes comprised four iron-binding components differing in electrophoretic mobility. This heterogeneity is not caused by sialic acid, which is absent. Amino acid composition, content of hexoses (1 mole/mole of protein) and hexosamines (1 mole/mole of protein), molecualr weight (70,000), and the isoelectric point (5.0) have been determined. No N-terminal amino acid could be detected.

Evaluation of non-specific immune components from the skin mucus of olive flounder (Paralichthys olivaceus)

“…3 [38e40]. In the present study, 7 bands ranging from 20 to 97 kDa were identified as transferrin precursor/transferrin by MALDI-TOF/MS analysis which is in parallel to previously reported 7 genetic variants of transferrin in carp (Cyprinus carpio L.) serum [39]. These identified protein bands, whether by genetic polymorphism or the degradation of native transferrin by the action of various proteolytic enzymes in the epidermal mucus or denaturants used in the assay, needs to be elucidated.…”

Isolation and characterization of transferrin from common carp (Cyprinus carpio L) seminal plasma

“…The described procedure is the first isolation method of Tf from fish seminal plasma. So far, only procedures for the isolation of Tf from carp serum were established [19,20]. In order to isolate carp serum Tf, Valenta et al [19] applied a fivestep procedure that consisted of gel filtration (Sephadex G150), desalting (Sephadex G25), ion-exchange chromatography (DEAE Sephadex), desalting and ion-exchange chromatography (CMSephadex C50).…”

Allelic discrimination, three-dimensional analysis and gene expression of multiple transferrin alleles of common carp (Cyprinus carpio L.)

“…Electrophoresis was carried out in running buffer (72 mM Tris, 26 mM boric acid) at 90 V for 30 min followed by 250 V for 5 h [18]. Under these circumstances a number of 6-7 different alleles for carp Tf have been identified and shown to retain the ability to bind iron [19]. Protein bands were stained for 1 h with 0.04% Coomassie Brilliant Blue dissolved in 3.5% perchloric acid.…”