Isolation and characterization of transferrin from common carp (Cyprinus carpio L) seminal plasma

Dietrich, Mariola A.; Żmijewski, Dominik; Karol, Halina; Hejmej, Anna; Bilińska, Barbara; Jurecka, Patrycja; Irnazarow, Ilgiz; Słowińska, Mariola; Hliwa, Piotr; Ciereszko, Andrzej

doi:10.1016/j.fsi.2010.02.015

Cited by 37 publications

(20 citation statements)

References 42 publications

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“…In mammals, TF is a glycoprotein with two N-glycosylation sites that are vital for iron transport (MacGillivray et al, 1982;O'Brien et al, 2007). However, the M. amblycephala TF lacked the potential N-glycosylation sites, which is in accord with that of other cyprinid fish (Dietrich et al, 2010;Stratil et al, 1983;Yang et al, 2004).…”

Section: Fig 2 Expression Of the M Amblycephala Tf (A) Andmentioning

confidence: 81%

“…Each lobe contains two subdomains that interact to form a deep and hydrophilic ion-binding site (Gomme et al, 2005). By sequestrating free iron and creating an environment with low level of iron, TF is able to restrict the infection of most pathogens (Dietrich et al, 2010). Under infection, TF is generally identified as an acute phase protein Neves et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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The Megalobrama amblycephala transferrin and transferrin receptor genes: Molecular cloning, characterization and expression during early development and after Aeromonas hydrophila infection

Ding

Zhao

et al. 2015

Developmental & Comparative Immunology

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Section: Fig 2 Expression Of the M Amblycephala Tf (A) Andmentioning

confidence: 81%

Section: Introductionmentioning

confidence: 99%

The Megalobrama amblycephala transferrin and transferrin receptor genes: Molecular cloning, characterization and expression during early development and after Aeromonas hydrophila infection

Ding

Zhao

et al. 2015

Developmental & Comparative Immunology

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“…Upon bacterial infection, TF and TFR can create a bacteriostatic environment by reducing the availability of iron to pathogens for proliferation [41,42]. In this study, we predicted that the differentially expressed miR-375 could bind to TF and TFR genes.…”

Section: Resultsmentioning

confidence: 99%

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Cui

Wei

et al. 2016

IJMS

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MicroRNAs (miRNAs) are small RNA molecules that play key roles in regulation of various biological processes. In order to better understand the biological significance of miRNAs in the context of Aeromonas hydrophila infection in Megalobrama amblycephala, small RNA libraries obtained from fish liver at 0 (non-infection), 4, and 24 h post infection (poi) were sequenced using Illumina deep sequencing technology. A total of 11,244,207, 9,212,958, and 7,939,157 clean reads were obtained from these three RNA libraries, respectively. Bioinformatics analysis identified 171 conserved miRNAs and 62 putative novel miRNAs. The existence of ten randomly selected novel miRNAs was validated by RT-PCR. Pairwise comparison suggested that 61 and 44 miRNAs were differentially expressed at 4 and 24 h poi, respectively. Furthermore, the expression profiles of nine randomly selected miRNAs were validated by qRT-PCR. MicroRNA target prediction, gene ontology (GO) annotation, and Kyoto Encylopedia of Genes and Genomes (KEGG) analysis indicated that a variety of biological pathways could be affected by A. hydrophila infection. Additionally, transferrin (TF) and transferrin receptor (TFR) genes were confirmed to be direct targets of miR-375. These results will expand our knowledge of the role of miRNAs in the immune response of M. amblycephala to A. hydrophila infection, and facilitate the development of effective strategies against A. hydrophila infection in M. amblycephala.

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“…Tf, mainly synthesized in the hypatocytes, is an iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids. The molecular mass of Tf has been determined to be around 74 KDa and isoelectric point about 5.1 (Dietrich et al, 2010). Therefore, in pH 7.4 PBS, Tf was negatively charged.…”

Section: Characterization Of Tf-9-nc-clmentioning

confidence: 99%

Characterization of 9-nitrocamptothecin-in-cyclodextrin-in-liposomes modified with transferrin for the treating of tumor

Chen

et al. 2015

International Journal of Pharmaceutics

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Isolation and characterization of transferrin from common carp (Cyprinus carpio L) seminal plasma

Cited by 37 publications

References 42 publications

The Megalobrama amblycephala transferrin and transferrin receptor genes: Molecular cloning, characterization and expression during early development and after Aeromonas hydrophila infection

The Megalobrama amblycephala transferrin and transferrin receptor genes: Molecular cloning, characterization and expression during early development and after Aeromonas hydrophila infection

Identification and Characterization of MicroRNAs in the Liver of Blunt Snout Bream (Megalobrama amblycephala) Infected by Aeromonas hydrophila

Characterization of 9-nitrocamptothecin-in-cyclodextrin-in-liposomes modified with transferrin for the treating of tumor

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