Polymorphism of TaSAP1-A1 and its association with agronomic traits in wheat

Abstract

TaSAP1, a member of the stress association protein (SAP) gene family from wheat, is involved in response to several abiotic stresses, including drought, salt and cold. In this study, TaSAP1-A1, a TaSAP1 member on chromosome 7A, and its flanking sequences were isolated. Polymorphism analysis indicated that the average nucleotide diversity (π) of the whole region was 0.00296. The highest nucleotide diversity occurred in the promoter region (π = 0.00631) and no polymorphism was identified in the coding region. Three markers T7AM5, T7AM2606 and T7AM39 located in the promoter region, were developed from sequence variations (InDel5-1810, SNP-2606 and InDel39-1637). Six haplotypes were identified among 300 accessions based on the three markers. TaSAP1-A1 was located on chromosome 7A using marker T7AM39 and was flanked by markers Xwmc530 and Xbarc174. QTL for yield-related traits, including 1,000-grain weight, number of grains per spike and grain yield, were located in the same region. In marker- and haplotype-trait association analyses, TaSAP1-A1 was significantly associated with 1,000-grain weight, number of grains per spike, spike length, peduncle length and total number of spikelets per spike in multiple environments. These results provide useful information for marker-assisted selection for yield-related traits under well-watered and drought-stressed conditions.

A dCAPS marker developed from a stress associated protein gene TaSAP7-B governing grain size and plant height in wheat

“…In the current study, polymorphism analysis of TaSAP7-B showed that the nucleotide diversity was found in the promoter region but not in coding region. Aforementioned result was in consistent with another SAP member in wheat, TaSAP1 (Chang et al 2013). TaSAP7-B was mapped on chromosome 5B flanked by and Xwmc380 (5.26 cM).…”

“…A mixed linear model (MLM) approach was applied in association analysis between TaSAP7-B allelic variation and agronomic traits to reduce false association effect caused by population structure. Previous studies indicated that TaSAP1 allelic variation significantly correlated with agronomic traits, such as 1 000-grain weight, spike length and number of spikelet per spike (Chang et al 2013(Chang et al , 2014. In this study, elite allele in TaSAP7-B gene was identified by the dCAPS marker and association analysis.…”

A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis

“…The TaSAP1 and TaSAP2 genes are found to be involved in multiple abiotic stress response, including drought, high salinity, cold and exogenous abscisic acid (Wang 2011). Six haplotypes (Hap) of TaSAP1-A1 have been identified in 300 wheat accessions, among them TaSAP1-A1-HapIII is significantly associated with thousand-grain weight in multiple growing environments (Chang et al 2013).…”

Genome-wide association for grain morphology in synthetic hexaploid wheats using digital imaging analysis

“…Further, the development of functional markers and cloning of genes relevant to grain weight have become major research focus in the past few years. Many QTLs for grain size and weight in rice have been fine-mapped and cloned in wheat including TaCwi-1A [32], TaSus2-2B [33], TaGw2-6A [34], TaCKX6-D1 [35], TaSap1-A1 [36], TaGS1-6D [37] and TaLsu1 [38]. …”