A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis

Xu, Qiao-fang; Mao, Xinguo; Wang, Yi-Xue; Wang, Jingyi; Xi, Yajun; Jing, Ruilian

doi:10.1016/s2095-3119(17)61681-2

Cited by 22 publications

(20 citation statements)

References 34 publications

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“…The specific role of AN1 domain is still poorly known. Two SAP proteins, including Arabidopsis AtSAP13 and T. aestivum TaSAP17-D, that contain two AN1 domains have been reported to enhance the salt tolerance in transgenic plants [33]. In our study, overexpression of PtSAP13 also improved salt tolerance of transgenic plants, as evidenced by the better growth in fresh weight and root length, as well as increased activities of antioxidative enzymes than WT plants under salt treatment.…”

Section: Discussionsupporting

confidence: 67%

“…Except for the function investigations of SAPs containing A20 and AN1 domains, AtSAP13 with two AN1 domains and one C2H2 domain is up-regulated under ABA, salt, and Cd stresses, and AtSAP13 -overexpressing plants exhibit high tolerance to drought, salt, and toxic metals [32]. Wheat TaSAP17 - D with two AN1 domains and two C2H2 domains is induced by salt, drought, and cold stresses, and it can enhance tolerance to salt stress in transgenic Arabidopsis [33]. Despite these advances, these studies mainly concentrated on herbaceous plants, and the biological functions of SAP genes in woody plants is far from complete.…”

Section: Introductionmentioning

confidence: 99%

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A Stress-Associated Protein, PtSAP13, From Populus trichocarpa Provides Tolerance to Salt Stress

Sun

Xia

et al. 2019

IJMS

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The growth and production of poplars are usually affected by unfavorable environmental conditions such as soil salinization. Thus, enhancing salt tolerance of poplars will promote their better adaptation to environmental stresses and improve their biomass production. Stress-associated proteins (SAPs) are a novel class of A20/AN1 zinc finger proteins that have been shown to confer plants’ tolerance to multiple abiotic stresses. However, the precise functions of SAP genes in poplars are still largely unknown. Here, the expression profiles of Populus trichocarpa SAPs in response to salt stress revealed that PtSAP13 with two AN1 domains was up-regulated dramatically during salt treatment. The β-glucuronidase (GUS) staining showed that PtSAP13 was accumulated dominantly in leaf and root, and the GUS signal was increased under salt condition. The Arabidopsis transgenic plants overexpressing PtSAP13 exhibited higher seed germination and better growth than wild-type (WT) plants under salt stress, demonstrating that overexpression of PtSAP13 increased salt tolerance. Higher activities of antioxidant enzymes were found in PtSAP13-overexpressing plants than in WT plants under salt stress. Transcriptome analysis revealed that some stress-related genes, including Glutathione peroxidase 8, NADP-malic enzyme 2, Response to ABA and Salt 1, WRKYs, Glutathione S-Transferase, and MYBs, were induced by salt in transgenic plants. Moreover, the pathways of flavonoid biosynthesis and metabolic processes, regulation of response to stress, response to ethylene, dioxygenase activity, glucosyltransferase activity, monooxygenase activity, and oxidoreductase activity were specially enriched in transgenic plants under salt condition. Taken together, our results demonstrate that PtSAP13 enhances salt tolerance through up-regulating the expression of stress-related genes and mediating multiple biological pathways.

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Section: Discussionsupporting

confidence: 67%

Section: Introductionmentioning

confidence: 99%

A Stress-Associated Protein, PtSAP13, From Populus trichocarpa Provides Tolerance to Salt Stress

Sun

Xia

et al. 2019

IJMS

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“…Numerous studies show that the plant SAP gene family is constitutively expressed. For example, the transcript of TaSAP17-D had been shown to be expression in various tissues at different development stages and higher expression in leaves at seedling stage (Xu et al 2018). In this study, expression analysis in different tissues at seedling stage showed that the expression levels of SiSAP4 were higher in leaves than in stems and roots, suggesting that it mainly played its biological role in leaves.…”

Section: Discussionmentioning

confidence: 51%

Foxtail Millet Stress Associated Protein Gene SiSAP4 Enhances Drought Stress Tolerance in Transgenic Arabidopsis

Li¹

2021

IJAB

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Abiotic stresses like drought affect plant growth and crop yield with climate change worsening. Stress associated proteins (SAPs), as the zinc finger proteins with A20/AN1 domain, play an important role in regulating abiotic stress response. As a typical summer dryland grain crop in the north of China, foxtail millet has the characteristics of drought resistance, making it a valuable resource for anti-stress gene exploitation and utilization. In this study, SiSAP4 gene was cloned from foxtail millet variety Yugu 1. Analysis showed that SiSAP4 gene was expressed in roots, stems and leaves at seedling stage, and the highest expression level was detected in leaves. Expression patterns under different stress conditions showed that expression level of SiSAP4 gene was significantly up-regulated under drought stress, suggesting it may be involved in drought stress response. Subcellular localization indicated that SiSAP4 was present in the nucleus and cytoplasm. It was revealed that SiSAP4 had no function in transcriptional activation in the yeast system. Overexpression of SiSAP4 in transgenic Arabidopsis resulted in enhanced tolerance to drought stress, which was simultaneously demonstrated by increased expression of a broad range of stress response genes. Based on those results, SiSAP4 has the potential to be used in transgenic breeding to improve drought stress tolerance in other crops. © 2021 Friends Science Publishers

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“…Likewise, overexpression of another rice SAP gene (OsiSAP8) in tobacco and rice also resulted in tolerance to multiple stresses such as salt, drought, and cold [22]. Enhanced stress tolerance was also observed in transgenic plants overexpressing different SAP genes, such as AlSAP from Aeluropus littoralis [23,24], LmSAP from the halophyte Lobularia maritima [25,26], AtSAP9 and AtSAP13 from Arabidopsis thaliana [19,27], TaSAP17-D from Triticum aestivum [28], and PtSAP13 from Populus trichocarpa [11]. In addition, OsSAP1-overexpressing transgenic tobacco plants also displayed an increase in disease resistance against virulent bacterial pathogens with the up-regulation of defense-responsive genes [29].…”

Section: Introductionmentioning

confidence: 99%

Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber

Lai

Zhou

Pan

et al. 2020

Plants

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Stress-associated proteins (SAPs) are a class of zinc finger proteins that confer tolerance to a variety of abiotic and biotic stresses in diverse plant species. However, in cucumber (Cucumis sativus L.), very little is known about the roles of SAP gene family members in regulating plant growth, development, and stress responses. In this study, a total of 12 SAP genes (named as CsSAP1-CsSAP12) were identified in the cucumber genome, which were unevenly distributed on six chromosomes. Gene duplication analysis detected one tandem duplication and two segmental duplication events. Phylogenetic analysis of SAP proteins from cucumber and other plants suggested that they could be divided into seven groups (sub-families), and proteins in the same group generally had the same arrangement of AN1 (ZnF-AN1) and A20 (ZnF-A20) domains. Most of the CsSAP genes were intronless and harbored a number of stress- and hormone-responsive cis-elements in their promoter regions. Tissue expression analysis showed that the CsSAP genes had a broad spectrum of expression in different tissues, and some of them displayed remarkable alteration in expression during fruit development. RT-qPCR results indicated that all the selected CsSAP genes displayed transcriptional responses to cold, drought, and salt stresses. These results enable the first comprehensive description of the SAP gene family in cucumber and lay a solid foundation for future research on the biological functions of CsSAP genes.

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A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis

Abstract: ScienceDirect A wheat gene TaSAP17-D encoding an AN1/AN1 zinc finger protein improves salt stress tolerance in transgenic Arabidopsis XU Qiao-fang 1, 2 , MAO Xin-guo 2 , WANG Yi-xue 2 , WANG Jing-yi 2 , XI Ya-jun 1 , JING Rui-lian 2

Cited by 22 publications

References 34 publications

A Stress-Associated Protein, PtSAP13, From Populus trichocarpa Provides Tolerance to Salt Stress

A Stress-Associated Protein, PtSAP13, From Populus trichocarpa Provides Tolerance to Salt Stress

Foxtail Millet Stress Associated Protein Gene SiSAP4 Enhances Drought Stress Tolerance in Transgenic Arabidopsis

Identification and Expression Analysis of Stress-Associated Proteins (SAPs) Containing A20/AN1 Zinc Finger in Cucumber

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