Polymorphism of PIT‐1, PRL and GH genes in dairy cattle of the Caucasian Brown breed bred in various natural ecological zones of the Republic of Dagestan, Russia

Оздемиров, А. А.; Selionova, Marina; Chizhova, L. N.; Хожоков, А. А.; Surzhikova, E. S.; Ramazanova, Dariga

doi:10.18470/1992-1098-2020-2-165-171

Ûg Ross.: èkol. razvit.

2020

DOI: 10.18470/1992-1098-2020-2-165-171

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Polymorphism of PIT‐1, PRL and GH genes in dairy cattle of the Caucasian Brown breed bred in various natural ecological zones of the Republic of Dagestan, Russia

А. А. Оздемиров

Marina Selionova²,

L. N. Chizhova³

et al.

Abstract: Северо-Кавказский Федеральный научный аграрный центр, Михайловск, Россия Контактное лицо Людмила Н. Чижова, доктор сельскохозяйственных наук, профессор, главный научный сотрудник лаборатории иммуногенетики и ДНК-технологий, ФГБНУ «Северо-Кавказский федеральный научный аграрный центр»; 356241, Россия, г. Михайловск, ул. Никонова, 49. Тел.

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Cited by 2 publications

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Polymorphism of genes CAST, GH, GDF9 of sheep of the Dagestan mountain breed

Оздемиров

Chizhova²,

Хожоков

et al. 2021

Ûg Ross.: èkol. razvit.

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Aim. To study the polymorphism of the genes calpastatin (CAST), somatotropin (GH) and differential growth factor (GDF9) of sheep of the Dagestan mountain breed.Material and Methods. Studies on the genetic typing of Dagestan mountain sheep according to CAST, GH and GDF9 genes were carried out in conditions of distant pasture farming in the Republic of Dagestan by the Genetics and Breeding Laboratory of the Federal Agrarian Scientific Centre of the Republic of Dagestan, an accredited laboratory of immunogenetics and DNA technologies of the All-Russian Research Institute of Sheep and Goat Breeding and a branch of the North Caucasus Federal Scientific Agrarian Centre. These investigations were undertaken by the PCR-RFLP method (polymyrase chain reaction - restriction fragment length polymorphism) on a Tersik four-channel programmable thermal cycler from DNA-Technologia (Russia) using specific primers synthesized in the SYNTHOL research and production laboratory (Moscow).Results. Carrying out PCR-RFLP revealed breed-specific alleles with different frequency of occurrence, which was: in the locus of the CAST gene - allele CASTN - 0.03; allele CASTM - 0.97; at the GH gene locus - GHA allele - 100.0, GHB allele - 0; in the locus of the GDF9 gene - the GDF9A allele - 0.25, the GDF9G allele - 0.75, which determined the frequency of occurrence of the homo- and heterozygous genotypes: CASTNN, CASTMM and CASTNM - 93.0; 0 and 7.0%; GHAA, GHBB and GHAB - 100.0 and 0%; GDF9AA, GDF9GG and GDF9АG- 16.0, 66.0 and 19.0%, respectively.Conclusion. The regularity revealed can be considered as an ecological factor that optimizes the adaptive functions of the sheep's organism, on the one hand, an evolutionary selection process that contributes to the creation of a specific way of genetic structures in the population, on the other. The assumption is made that the information obtained can serve as the beginning of a more in-depth study of the unique gene pool of sheep of the Dagestan rock breed for its further improvement. Variants of genetic marker profiles of parental pairs are proposed for accumulation in breeding herds bred in different ecological-geographical zones and genotypes of carriers of selection-significant genetic structures.

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Polymorphism of genes CAST, GH, GDF9 of sheep of the Dagestan mountain breed

Оздемиров

Chizhova²,

Хожоков

et al. 2021

Ûg Ross.: èkol. razvit.

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Analysis of polymorphisms in genes associated with milk productivity in red steppe and Caucasian brown cow breeds in two natural‐climate zones (mountains and plain) of Dagestan, Russia

Ozdemirov,

Khozhokov,

Huseynova

et al. 2023

Ûg Ross.: èkol. razvit.

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Aim. The unique gene pool of indigenous breeds in the Dagestan Caucasus is threatened by the increasingly widespread use of commercial breeds, which in turn risks narrowing the genetic base necessary to increase and maintain existing genetic diversity. For this reason, our research aimed to study the polymorphism of the PRL, PIT‐1, GH genes in red steppe and Caucasian brown cattle raised in the conditions of Dagestan.Methods. Genotyping of red steppe and Caucasian brown cattle was carried out using PCR‐RFLP methods. Using these methods, genetic analysis was carried out and polymorphism of the prolactin, somatotropin and pituitary‐specific transcription factor genes was studied.Results. As a result of the genotyping of an experimental group of red steppe cattle, it was found that the distribution of two alleles and three genotypes (PIT‐1A and PIT‐1B; PIT‐1AA, PIT‐1BB, PIT‐1AB) of the PIT‐1 gene has a specific character. The specificity of the allelic spectrum of the PRL gene is expressed in the high (0.84) frequency of occurrence of the PRLA allele and low (0.16) frequency of the PRLB allele. This is reflected in the presence of homo‐ and heterozygous genotypes with a frequency of occurrence of 73.0; 23.0; 4.0 %, respectively.Conclusion. Applied genetic approaches to improve the productive and breed qualities of livestock, which are modern and based on a more complete analysis of the genetic diversity and genotype of the studied populations, contribute to the conservation of zoned breeds. At the same time, intrapopulation variability and good adaptability should ensure the sustainable development of dairy cattle breeding in various climatic zones.

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Polymorphism of PIT‐1, PRL and GH genes in dairy cattle of the Caucasian Brown breed bred in various natural ecological zones of the Republic of Dagestan, Russia

Cited by 2 publications

References 6 publications

Polymorphism of genes CAST, GH, GDF9 of sheep of the Dagestan mountain breed

Polymorphism of genes CAST, GH, GDF9 of sheep of the Dagestan mountain breed

Analysis of polymorphisms in genes associated with milk productivity in red steppe and Caucasian brown cow breeds in two natural‐climate zones (mountains and plain) of Dagestan, Russia

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