Polymorphism of <i>DGAT1</i> associated with intramuscular fat‐mediated tenderness in sheep

Qiu-liang, XU; Chen, Yulin; Ma, Ruixia; Xue, Peng

doi:10.1002/jsfa.3431

Cited by 27 publications

(30 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It has been previously reported in other studies at various sheep breeds by Xu et al (2008) These studies were performed on various individuals and several mutations were found besides the one revealed in our study in exon 17. These author concluded also that there is no signi icant association with the milk fat content.…”

Section: Resultssupporting

confidence: 80%

“…These author concluded also that there is no signi icant association with the milk fat content. However, Xu et al (2008) has revealed a signi icant association of this polymorphism with the IMF content, muscle marbling score and meat tenderness. The authors have concluded that the T allele, which was found in a lesser frequency in our study, had a positive effect on sheep meat quality traits.…”

Section: Resultsmentioning

confidence: 99%

“…In sheeps and goats the DGAT1 K232A polymorphism has not been so extensively studied, although the essential role of DGAT1 in milk fat content genetic variation makes this marker an important factor for the variety of milk traits found in individuals (Scata et al, 2009;Xu et al, 2008). The previous work done by Scata et al (2009) on possible DGAT1 polymorphism revealed a single nucleotide polymorphism (SNP) in the 5'UTR of DGAT1 woth possible in luence on milk fat percent.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification of Polymorphism in Goat and Sheep DGAT1 Gene Associated with Milk Production Traits

Tăbăran¹,

Mihaiu²,

Dan³

et al. 2014

BUASVMCN-VM

View full text Add to dashboard Cite

Diacylglycerol acyltransferase 1 (DGAT1) is considered a key marker in milk production traits at bovines. Recently, several studies were conducted on sheeps and goats to reveal possible single nucleotide polymorpshims (SNPs) that might in luence the production and could help in individual selection. The study was conducted on 50 ewes from the Turcana breed and 60 goats from the Carpatian breed rasied in Transylvania mountain area. The method applied was PCR-RFLP with AluI enzyme. The results revealed no polymorphism in exon 14-16 at Carpatian goats, leading us to the conclusion that sequencying should be applied as method for further SNP detection. In exon 17 a previously reported mutation (C-T) was identi ied and three corresponding genotypes (CC, CT and TT). No statistically signi icant differences were found among these three genotypes in what concerns the fat percent in milk. We concluded that further investigations are needed in DGAT1 gene to detect possible SNP associated with milk production traits in these species.

show abstract

Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Polymorphism in Goat and Sheep DGAT1 Gene Associated with Milk Production Traits

Tăbăran¹,

Mihaiu²,

Dan³

et al. 2014

BUASVMCN-VM

View full text Add to dashboard Cite

show abstract

“…They also include the thyroglobulin (TG) gene in cattle (Barendse, 2002), the diacylglycerol acyltransferase 1 or 2 (DGAT1; DGAT2) genes in cattle (Thaller et al, 2003), pigs (Nonneman and Rohrer, 2002), chicken (Bourneuf et al, 2006) and sheep (Xu et al, 2009), and the splicing factor serine-arginine-rich protein (SFRS18) gene in pigs (Wang et al, 2009b). However, the results are often inconsistent.…”

Section: Genetic Markersmentioning

confidence: 99%

Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers

Hocquette

Gondret

Baéza³

et al. 2010

Animal

637

447

View full text Add to dashboard Cite

Intramuscular fat (IMF) content plays a key role in various quality traits of meat. IMF content varies between species, between breeds and between muscle types in the same breed. Other factors are involved in the variation of IMF content in animals, including gender, age and feeding. Variability in IMF content is mainly linked to the number and size of intramuscular adipocytes. The accretion rate of IMF depends on the muscle growth rate. For instance, animals having a high muscularity with a high glycolytic activity display a reduced development of IMF. This suggests that muscle cells and adipocytes interplay during growth. In addition, early events that influence adipogenesis inside the muscle (i.e proliferation and differentiation of adipose cells, the connective structure embedding adipocytes) might be involved in interindividual differences in IMF content. Increasing muscularity will also dilute the final fat content of muscle. At the metabolic level, IMF content results from the balance between uptake, synthesis and degradation of triacylglycerols, which involve many metabolic pathways in both adipocytes and myofibres. Various experiments revealed an association between IMF level and the muscle content in adipocyte-type fatty acid-binding protein, the activities of oxidative enzymes, or the delta-6-desaturase level; however, other studies failed to confirm such relationships. This might be due to the importance of fatty acid fluxes that is likely to be responsible for variability in IMF content during the postnatal period rather than the control of one single pathway. This is evident in the muscle of most fish species in which triacylglycerol synthesis is almost zero. Genetic approaches for increasing IMF have been focused on live animal ultrasound to derive estimated breeding values. More recently, efforts have concentrated on discovering DNA markers that change the distribution of fat in the body (i.e. towards IMF at the expense of the carcass fatness). Thanks to the exhaustive nature of genomics (transcriptomics and proteomics), our knowledge on fat accumulation in muscles is now being underpinned. Metabolic specificities of intramuscular adipocytes have also been demonstrated, as compared to other depots. Nutritional manipulation of IMF independently from body fat depots has proved to be more difficult to achieve than genetic strategies to have lipid deposition dependent of adipose tissue location. In addition, the biological mechanisms that explain the variability of IMF content differ between genetic and nutritional factors. The nutritional regulation of IMF also differs between ruminants, monogastrics and fish due to their digestive and nutritional particularities.

show abstract

“…These genes are involved in muscle development, growth (Nonneman and Koohmaraie, 1999) as well as in eating quality (tenderness) of the mutton (Byun et al 2009). The DGAT1 and FABP3 are involved in fatty acid metabolism and associated with milk fat content and marbling (Scata et al 2009;Xu et al 2009). It is imperative that knowledge be acquired of the available Indian sheep populations which are as yet untouched reservoirs of potential genes defining mutton quality.…”

Section: Introductionmentioning

confidence: 99%

Identification of novel single nucleotide polymorphisms in candidate genes for mutton quality in Indian sheep

Arora

Yadav²,

Yadav³

2014

amb

View full text Add to dashboard Cite

The study reports the identification of novel SNPs in candidate genes for mutton quality across a panel of eleven phenotypically and geographically diverse Indian sheep breeds. The investigated candidate genes were CAPN4, CAST, FABP3 and DGAT1 which are known to be polymorphic. Nine novel SNPs could be identified across the studied gene loci which were in the heterozygous condition. One SNP in the exon 1 of DGAT1 was non-synonymous. The average expected heterozygosity determined in the eleven Indian sheep breeds at these loci was 0.328. The allele and genotype frequencies for all the identified SNPs were determined. The average haplotype and nucleotide diversity across all loci were 0.545 and 0.206 respectively. The information generated provides preliminary indication of the functional diversity present in Indian sheep. Future studies will be required to establish the effect of the reported SNPs in the Indian sheep populations.

show abstract

Polymorphism of DGAT1 associated with intramuscular fat‐mediated tenderness in sheep

Cited by 27 publications

References 32 publications

Identification of Polymorphism in Goat and Sheep DGAT1 Gene Associated with Milk Production Traits

Identification of Polymorphism in Goat and Sheep DGAT1 Gene Associated with Milk Production Traits

Intramuscular fat content in meat-producing animals: development, genetic and nutritional control, and identification of putative markers

Identification of novel single nucleotide polymorphisms in candidate genes for mutton quality in Indian sheep

Contact Info

Product

Resources

About