Polymorphism of High MrGlutenin Subunits in Triticum tauschii: Characterisation by Chromatography and Electrophoretic Methods

Gianibelli, M.C.; Gupta, Ram B.; Lafiandra, D.; Margiotta, B.; MacRitchie, Finlay

doi:10.1006/jcrs.2000.0328

Cited by 75 publications

(49 citation statements)

References 31 publications

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“…high technological quality, whereas their allelic variants such as Glu A1 (null), Glu B1 (6 + 8), or Glu D1 (2 + 12) are related to lower quality (Gıanıbellı et al, 2001). Judging by the HMW-GS composition, only one genotype (G7) is expected to have good quality, due to the presence of subunits 2*, 5+10, and 17+18 (Payne et al, 1987).…”

Section: Biplot Analysismentioning

confidence: 99%

Effects of genotype and environment on grain yield and quality traits in bread wheat (T. aestivum L.)

Kaya¹,

Akçura²

2014

Food Sci. Technol (Campinas)

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Section: Biplot Analysismentioning

confidence: 99%

Effects of genotype and environment on grain yield and quality traits in bread wheat (T. aestivum L.)

Kaya¹,

Akçura²

2014

Food Sci. Technol (Campinas)

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“…HMW-GS identification by CE was achieved through comparison of single and mixture samples according to standard cultivars and the results obtained from SDS-PAGE with various gel concentrations as well as from previous reports [15,24]. The novel subunits identified in this study were designated according to the previous methods [27,28].…”

Section: Hmw-gs Identification and Nomenclaturementioning

confidence: 99%

Capillary electrophoresis separation of high molecular weight glutenin subunits in bread wheat (Triticum aestivum L.) and related species with phosphate‐based buffers

Yan

Jiang

et al. 2003

Electrophoresis

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This study focused on optimizing phosphate-based buffers and other capillary electrophoresis (CE) parameters for separating and characterizing high molecular weight glutenin subunits (HMW-GS) in bread wheat (Triticum aestivum L., AABBDD, 2n = 6x = 42), emmer (Triticum dicoccum, AABB, 2n = 4x = 28) and Aegilops tauschii (DD, 2n = 2x = 14). The fast and high-resolution separation of HMW-GS was achieved using 0.1 M phosphate-glycine buffer (pH 2.5, containing 20% acetonitrile and 0.05% hydroxypropylmethylcellulose) at 12.5 kV and 40 degrees C with 25 microm inside diameter (ID)x27 cm uncoated fused-silica capillary. In general, one sample separation can be analyzed in 15 min. The good run-to-run repeatable separation of HMW-GS could be obtained with a relative standard deviation of less than 1% when capillaries were rinsed with 1 M phosphoric acid for 2 min, followed by separation buffer for 2 min after each separation. The HMW-GS from some bread wheat cultivars as well as tetraploid and diploid accessions was separated by the CE method described above, and all subunits detected were well characterized and readily identified. Some HMW-GS showed reversed mobilities and elution order compared to the methods of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and SDS-CE. Particularly, most of the HMW-GS analyzed with the CE buffer used were separated into multiple peaks, generally a high peak plus a minor peak. CE appears to be capable of separating and characterizing HMW-GS with fast and high-resolution features, therefore it is expected to be useful for specific germplasm screening and desirable HMW-GS identification in wheat quality improvement.

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“…In fact, some studies have reported higher levels of genetic variability of glutenin subunits and gliadin in Ae. tauschii than in the D genome of common wheat (Gianibelli et al 2001, 2002, Yan et al 2003a, 2003b, Giles and Brown 2006. Ae.…”

Section: Introductionmentioning

confidence: 97%

Natural variation of morphological traits in wild wheat progenitor Aegilops tauschii Coss.

et al. 2009

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Aegilops tauschii Coss. (syn Ae. squarrosa L.) is a wild diploid wheat species. It has a wide natural species range in central Eurasia, spreading from northern Syria and Turkey to western China. Ae. tauschii is known as the D genome progenitor of hexaploid bread wheat. The genealogical and geographical structure of variation of morphological traits was analyzed using a diverse array of 205 sample accessions that represented the entire species range. In total, 27 traits, including anther and pistil shape and internode length, were examined in this study. Large-scale natural variation was found for all examined traits. Geographically, significant longitudinal clines were detected for anther size, internode length and spike size and shape. Anthers tended to be small in accessions from the eastern region. Internodes also tended to be short, whereas spikes tended to be long in accessions from the eastern region. Spikelet density per spike tended to be high in the eastern habitats. In the process of west-to-east dispersal, Ae. tauschii underwent extensive morphological, genetic and ecological diversification that produced the variation seen among today's natural populations.

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Polymorphism of High MrGlutenin Subunits in Triticum tauschii: Characterisation by Chromatography and Electrophoretic Methods

Cited by 75 publications

References 31 publications

Effects of genotype and environment on grain yield and quality traits in bread wheat (T. aestivum L.)

Effects of genotype and environment on grain yield and quality traits in bread wheat (T. aestivum L.)

Capillary electrophoresis separation of high molecular weight glutenin subunits in bread wheat (Triticum aestivum L.) and related species with phosphate‐based buffers

Natural variation of morphological traits in wild wheat progenitor Aegilops tauschii Coss.

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