Polymorphism of growth hormone gene in three goat breeds in Egypt

Abstract

Background: In Egypt, like other developing countries, goats are prime resources of meat. So, selection of goats for superior growth rate is advantageous. Growth hormone (GH) is the main regulator of animal growth, and encoded by GH gene that exhibits active gene variants improving growth. The objective of this study was to identify GH gene variants in three goat breeds (Barki, Damascus, and Zaraibi), via polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and gene sequencing analyses. Results: Three loci on GH gene named GH1, GH2, and GH6 polymorphisms were analyzed. GH1-HaeIII/RFLP showed only two genotypes (AB and BB) in all breeds, with absent AA genotype. Both Barki and Zaraibi exhibited the highest GC genotype frequency (0.95). GH2-HaeIII/RFLP produced only two homozygous genotypes AA in Damascus and BB in both Barki and Zaraibi, with the absence of AB genotype in the three breeds. However, digestion of GH6 by HaeIII was monomorphic; it exhibited different single-nucleotide polymorphisms (SNPs), detected by DNA sequencing, among the studied goat breeds. Conclusions: The revealed SNPs could be employed as useful markers, helping goat breeders in selection of goats for high growth performance. Further analyses with larger sample size are needed for investigating the relationship between the different genotypes and growth traits.

Polymorphism of Growth Hormone (GH) Gene in Lakor Goat from Lakor Island of Southwest Maluku Regency

“…The result of this study has similar with previous studies, in Chengdu-Ma goat from China (Bai et al 2005), Boer goat (Hua et al 2009), Savana and Kalahari goat from Malaysia (Marini et al 2012), Kacang goat from Indonesia (Ilham et al 2016), and Saburai goat from Indonesia (Sulastri et al, 2017). Conversely to the other studies, Mahrous et al (2018), reported that BB genotype was detected 5-10% and absence of AA genotype of three breed goat (Barki, Damascus and Zaraibi) from Egypt. In India, Barbari and Sirohi goat were detected AB (82% and 90%, respectively) and BB (18% and 10%, respectively) genotypes (Singh et al 2015).…”

Single-nucleotide polymorphisms in FABP4 gene associated with growth traits in Egyptian sheep

“…Progress in molecular biology in the past three decades has made possible identifying genomic regions responsible for phenotypic variation in meat production traits. Several reports on SNP associations with meat traits have emerged in various livestock species [11][12][13][14][21][22][23]. We investigated polymorphisms in intron 1 of the Egyptian ovine FABP4 gene and assessed the potential association of SNPs in this region of the gene with growth traits.…”

Genetic variability in IGFBP-3 and GH genes and their association with body weight and growth performance at birth, weaning and six-month in sheep 

“…* O = Other studied sheep breeds, **RXB = Rahmani X Barki cross, ***SC = Awassi X Suffolk cross Growth performance, body weight and body size are important indicators of body growth and some other breeding traits as well (Okoro et al, 2010). Mahrous et al, (2018) studied the polymorphism of GH gene for the three loci; GH-1, -2, and -6 in three goat breeds; Damascus, Barki, and Zaraibi, utilizing RFLP and DNA analyses and confirmed the absence AA of genotype, which all animals had genotypes BB or AB. Worth mentioning, An et al, (2011) when investigated the polymorphisms of GH gene in Boer (BG) and Xinong Saanen (SG) goats found two alleles (A and B) and three genotypes (AA, AB and BB) using SSCP.…”