Lakor goat is a domestic animal from Lakor island in South West Maluku Regency. They successfully survive and reproduce with limited water, grass and high temperature. Growth traits is an economic trait which important for breeding strategy (productivity improvement). Pituitary Specific Transcription Factor 1 (POU1F1) is otherwise known as PIT1 gene plays a role of growth and carcass traits. The objective of this study was to identify the POU1F1 gene in Lakor goat from Lakor island in Southwest Maluku Regency. Sixty-three samples of hair tail were collected from three different locations: Ketti Letpey (18), Werwawan Jamluli (26) and Letoda (19). DNA was extracted by DNA isolation kit. POU1FI gene was amplified using PCR technique while genotyping used PCR-RFLP with PstI restriction enzyme. Result showed that only one genotype (TT) was found and monomorphic. It may be caused remote area and limited of ram that indicated inbreeding has occurred. Inbreeding rate or coefficient study and introgression of ram from out of Lakor island by Artificial Insemination (AI) or natural mating is needed.
This study aimed to analyze borax content in food and the effects of food processing on the content of borax in food. This research adopted a survey method. Food samples were obtained from the traditional and modern markets, elementary schools, and supermarkets available in the region of Pamulang Subdistrict. Sampling was done by using a purposive technique. Each sample was qualitatively analyzed for its borax content using a borax test kit. The detected food was then analyzed by using a qualitative analysis (i.e., there are four categories of data); one sample per category was taken to be analyzed quantitatively using the HPLC method in the Laboratorium of Balai Besar Pusat Penelitian dan Pengembangan Pascapanen Pertanian, Bogor. The treatment of food containing the highest level of borax) was conducted by boiling them during a certain period of time (i.e., 5 minutes, 15 minutes, and 30 minutes) and then frying them until well cooked (was not burnt). The collected data were then analyzed descriptively. The results showed that 54% of the samples obtained from the Elementary Schools positively contained borax; as much as 74% of the food samples obtained from traditional markets contained borax positively; while all samples coming from the supermarkets did not contain borax. The content of borax in the detected food in this research ranged between 560 mg/kg up to 17,640 mg/kg. The highest content of borax found in yellow wet noodles (17,640 mg/kg) was far above the maximum level specified by EFSA (2013), that is as much as 4,000 mg/kg. The boiling process was quite effective in lowering the level of borax in food, while the frying process did not actually reduce the level of borax in food. Penelitian ini bertujuan untuk menganalisis kandungan boraks pada makanan dan pengaruh pengolahan makanan terhadap kandungan boraks pada makanan. Penelitian ini dilakukan dengan metode survei. Jenis makanan yang dijadikan sampel dalam penelitian ini adalah jenis makanan yang diperoleh dari pasar tradisional dan modern, sekolah dasar, dan supermarket di wilayah Kecamatan Pamulang. Pengambilan sampel dilakukan dengan menggunakan teknik purposive sampling. Setiap sampel dianalisis kandungan boraksnya secara kualitatif menggunakan test kit boraks. Selanjutnya, makanan yang telah terdeteksi oleh analisis kualitatif (ada empat kategori data), diambil satu sampel per kategori untuk dianalisis secara kuantitatif menggunakan metode HPLC di Laboratorium Balai Besar Pusat Penelitian dan Pengembangan Pascapanen Pertanian, Bogor. Makanan yang terdeteksi mengandung boraks paling tinggi diperlakukan dengan cara direbus dalam beberapa rentang waktu (5 menit, 15 menit, dan 30 menit) dan digoreng sampai matang (tidak sampai gosong). Data yang dikumpulkan dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa 54% dari sampel makanan yang diperoleh dari Sekolah Dasar positif mengandung boraks; sebanyak 74% dari sampel makanan yang diperoleh dari pasar tradisional positif mengandung boraks; sementara semua sampel berasal dari supermarket tidak terdeteksi mengandung boraks. Kandungan boraks dalam makanan yang terdeteksi dalam penelitian ini berkisar antara 560 mg/kg sampai dengan 17.640 mg/kg. Kandungan tertinggi ditemukan dalam mie basah kuning (17.640 mg/kg) jauh di atas kadar maksimum yang ditentukan EFSA (2013) sebanyak 4.000 mg/kg. Proses perebusan cukup efektif dalam menurunkan kadar boraks dalam makanan, sedangkan proses penggorengan tidak mengurangi kadar boraks dalam makanan.
Maluku is known by the nickname "The Spice Island". This nickname is given based on the Maluku geoevolution process derived from the Indo-Australian plates with the abundant distribution and composition of flora and fauna. Manusela National Park (MNP) is a tropical rainforest located on the Seram island. Maluku Province based on the distribution of biogeography categorized in the Wallacea zone that has high species endemicity, one of which is Salmon-Crested Cockatoo (C. moluccensis). This study aims to identify the species of trees that serve as feedsources and nesting places from Salmon-Crested Cockatoo (C. moluccensis) as Seram endemic species in MNP. This ecological information is very important for designing strategies and development of appropriate in-situ conservation units for the sustainability of these wildlife in their natural habitat. This study was conducted for one month, from November to December 2017 in MNP. The results of this study indicate that found fives species of spermatophyta trees that serves as feed sources and Fours species that serve as nesting places of C. moluccensis. The trees species that serve as feed sources include C. vulgare, E. rumphii, H. globularia, C. soulattri, and Callamus sp. while which serves as nesting places such as T. copelandii, N. moluccana, O. sumatrana, and P. goajava.
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