Polymorphism of full-length copies of the transposable element MDG4 (gypsy) cloned from the Drosophila melanogaster strain G32

Salenko, V. B.; Котнова, А. П.; Karpova, Nina N.; Lyubomirskaya, N. V.; Ilyin, Yu. V.

doi:10.1134/s1607672907010097

Cited by 4 publications

(2 citation statements)

References 8 publications

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“…To synthesize the final UAS-WM, the myr-GFP-V5 and H2B-mCherry-HA products were linked with P2A sequence [64, 65] and then inserted into the MCS of pUAST with NotI and XhoI digestion (see S1 Fig for full WM sequences). The gypsy backbone was obtained as a gift from V. Salenko [75]. In order to generate gypsy-CLEVR reporter, gypsy backbone that was placed in the pCaSpeR5 transforming vector [76], and the 5xUAS regulatory element from pUAST plasmid were inserted into the BglII site of the U5 domain within gypsy 5′LTR, with antisense orientation relative to gypsy backbone.…”

Section: Methodsmentioning

confidence: 99%

Cellular labeling of endogenous retrovirus replication (CLEVR) reveals de novo insertions of the gypsy retrotransposable element in cell culture and in both neurons and glial cells of aging fruit flies

et al. 2019

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Evidence is rapidly mounting that transposable element (TE) expression and replication may impact biology more widely than previously thought. This includes potential effects on normal physiology of somatic tissues and dysfunctional impacts in diseases associated with aging, such as cancer and neurodegeneration. Investigation of the biological impact of mobile elements in somatic cells will be greatly facilitated by the use of donor elements that are engineered to report de novo events in vivo. In multicellular organisms, reporter constructs demonstrating engineered long interspersed nuclear element (LINE-1; L1) mobilization have been in use for quite some time, and strategies similar to L1 retrotransposition reporter assays have been developed to report replication of Ty1 elements in yeast and mouse intracisternal A particle (IAP) long terminal repeat (LTR) retrotransposons in cultivated cells. We describe a novel approach termed cellular labeling of endogenous retrovirus replication (CLEVR), which reports replication of the gypsy element within specific cells in vivo in Drosophila . The gypsy-CLEVR reporter reveals gypsy replication both in cell culture and in individual neurons and glial cells of the aging adult fly. We also demonstrate that the gypsy-CLEVR replication rate is increased when the short interfering RNA (siRNA) silencing system is genetically disrupted. This CLEVR strategy makes use of universally conserved features of retroviruses and should be widely applicable to other LTR retrotransposons, endogenous retroviruses (ERVs), and exogenous retroviruses.

show abstract

Section: Methodsmentioning

confidence: 99%

Cellular labeling of endogenous retrovirus replication (CLEVR) reveals de novo insertions of the gypsy retrotransposable element in cell culture and in both neurons and glial cells of aging fruit flies

et al. 2019

View full text Add to dashboard Cite

show abstract

“…To synthesize the final UAS-WM, the myr-GFP-V5 and H2B-mCherry-HA products were linked with P2A sequence [59,60] and then inserted into the MCS of pUAST with NotI and XhoI digestion (see S1A Fig for full WM sequences). The gypsy backbone was obtained as a gift from V. Salenko [70]. In order to generate gypsy-CLEVR reporter, gypsy backbone that was placed in the pCaSpeR5 transforming vector [71], and the 5xUAS regulatory element from pUAST plasmid was inserted into the BglII site of U5 domain within gypsy 5'-LTR with antisense orientation relative to gypsy backbone.…”

Section: Constructsmentioning

confidence: 99%

Cellular labeling of endogenous virus replication (CLEVR) reveals de novo insertions of the gypsy endogenous retrovirus in cell culture and in both neurons and glial cells of aging fruit flies

Chang

Keegan

Prazak

et al. 2018

Preprint

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Evidence is rapidly mounting that transposable element expression and replication may impact biology more widely than previously thought. This includes potential effects on normal physiology of somatic tissues and dysfunctional impacts in diseases associated with aging such as cancer and neurodegeneration. Investigation of the biological impact of mobile elements in somatic cells will be greatly facilitated by use of donor elements that are engineered to report de novo events in vivo. In multicellular organisms, successful reporters of LINE element mobilization have been in use for some time, but similar strategies have not been developed to report Long Terminal Repeat (LTR) retrotransposons and endogenous retroviruses. We describe Cellular Labeling of Endogenous Virus Replication (CLEVR), which reports replication of the gypsy element in Drosophila. The gypsy-CLEVR reporter reveals gypsy replication both in cell culture and in individual neurons and glial cells of the aging adult fly. We also demonstrate that the gypsy-CLEVR replication rate is increased when the short interfering RNA silencing system is genetically disrupted. This CLEVR strategy makes use of universally conserved features of retroviruses and should be widely applicable to other LTR-retrotransposons, endogenous retroviruses and exogenous retroviruses.

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A rare family of gtwin retrotransposon carrying a mutation in the tRNA-primer binding site is amplified in G-32 Drosophila melanogaster strain

Salenko

Котнова

Глухов

et al. 2011

Dokl Biochem Biophys

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Polymorphism of full-length copies of the transposable element MDG4 (gypsy) cloned from the Drosophila melanogaster strain G32

Cited by 4 publications

References 8 publications

Cellular labeling of endogenous retrovirus replication (CLEVR) reveals de novo insertions of the gypsy retrotransposable element in cell culture and in both neurons and glial cells of aging fruit flies

Cellular labeling of endogenous retrovirus replication (CLEVR) reveals de novo insertions of the gypsy retrotransposable element in cell culture and in both neurons and glial cells of aging fruit flies

Cellular labeling of endogenous virus replication (CLEVR) reveals de novo insertions of the gypsy endogenous retrovirus in cell culture and in both neurons and glial cells of aging fruit flies

A rare family of gtwin retrotransposon carrying a mutation in the tRNA-primer binding site is amplified in G-32 Drosophila melanogaster strain

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