Polymorphism of ceramide 6: a vibrational spectroscopic and X-ray powder diffraction investigation of the diastereomers of N-(α-hydroxyoctadecanoyl)-phytosphingosine

Raudenkolb, Steve; Wartewig, S.; Neubert, Reinhard H.H.

doi:10.1016/j.chemphyslip.2004.09.015

Cited by 42 publications

(19 citation statements)

References 20 publications

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“…However, the repeat distance of 41.9 does not fully correspond with the values obtained for pure Cer[AP] [47]. It can be caused by the fact that in our case a small amount of CHOL is also present in the S-phase.…”

Section: Evaluation Methodscontrasting

confidence: 72%

Influence of cholesterol on the structure of stratum corneum lipid model membrane

Zbytovská

Киселев

Funari³

et al. 2008

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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a b s t r a c tThis study describes the influence of cholesterol on a model membrane consisting of four stratum corneum (SC) lipids in three different states at 32 and 85 • C. Using small-angle X-ray diffraction on multilamellar vesicles, the lamellar repeat distance, D, was determined. Small-angle neutron scattering on unilamellar vesicles was used to calculate the membrane thickness, average area of the membrane surface per molecule, average volume per molecule in the membrane, and membrane density. After the sample preparation, the membranes show one lamellar phase. Over 10 days, the systems separate into two lamellar phases and crystalline cholesterol. On heating the samples to 85 • C, the domains merge into one phase, the D-value of which increases slightly with increasing cholesterol concentration. On cooling the samples back to 32 • C, only one phase is observable again. The increasing cholesterol concentration in the membrane causes a decrease in the D-value, membrane thickness, and membrane density. Simultaneously, the area of the membrane surface and membrane volume per molecule increase. In conclusion, cholesterol fluidises the SC lipid membranes in the state below the main phase transition and condenses above it. This effect can have an important impact on the pathologic skin states such as the recessive X-linked ichthyosis.

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Section: Evaluation Methodscontrasting

confidence: 72%

Influence of cholesterol on the structure of stratum corneum lipid model membrane

Zbytovská

Киселев

Funari³

et al. 2008

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Evidently, these changes in the lamellar repeat distance are associated with the crystallization of ice. This finding indicates that water has to be located between the lamellar sheets and demonstrates the differences from ceramides of the phytosphingosine type, in which water cannot be incorporated into the structure (Raudenkolb et al, 2003b(Raudenkolb et al, , 2005.…”

Section: Hydrated Cer[as]mentioning

confidence: 88%

Hydration properties of N-(α-hydroxyacyl)-sphingosine: X-ray powder diffraction and FT–Raman spectroscopic studies

Raudenkolb

Wartewig²,

Brezesinski

et al. 2005

Chemistry and Physics of Lipids

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“…When in an extended conformation, the lipids can adjust the total length by adjusting the lipid angle along the basal plane of the lamellar phase. Angled linear CER conformations have been proposed for CER nonhydroxy phytosphingosine (41,42) and -hydroxy phytosphingosine (43).…”

Section: Conformation Of Cer Nsmentioning

confidence: 99%

Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model

Beddoes

Gooris

Bouwstra

2018

Journal of Lipid Research

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With the use of model systems, previous work has identified the relative positions of several of the lipid subclasses (17)(18)(19). CERs with a nonhydroxyl C24 acyl chain linked to a C18 sphingosine (NS) and FFAs were located in the central region of the LPP trilayer structure, while CHOL remained exclusively in the outer region (18). CER EOS is uniquely positioned in both the outer and interior regions, with the head groups present at the boundary of the unit cell, while the carbon chain extends to the central head Abstract The lipid matrix of the stratum corneum, the outermost skin layer, consists primarily of ceramides, cholesterol, and FFAs. These lipids form a trilayer long-periodicity phase (LPP) that is unique to this barrier. Knowledge about the LPP is essential in understanding the barrier function. Previous studies of LPP lipid models have identified the position of the major lipid classes and suggested that a large fraction of FFAs and the ceramide acyl chain are present in the central region. However, the precise arrangement, such as lipid subclass mixing (isolated or mixed) and ceramide conformation (extended or hairpin), remains unknown. Here, we deuterated FFAs and the ceramide acyl chain to study CD 2 and CH 2 interactions with Fourier-transform infrared spectroscopy. The ceramide and FFAs of various chain lengths were not in separate domains but had mixed together. The larger number of CD 2 -CD 2 lipid chain interactions in the LPP than in a symmetrical bilayer structure implied that the ceramide had primarily adopted an extended conformation. Shorter FFAs were present in the central region of the LPP. This model explores the biophysical properties of the stratum corneum's LPP to improve the understanding of the barrier function of this layer.-Beddoes, C. M., G. S. Gooris, and J. A. Bouwstra. Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model.

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Polymorphism of ceramide 6: a vibrational spectroscopic and X-ray powder diffraction investigation of the diastereomers of N-(α-hydroxyoctadecanoyl)-phytosphingosine

Cited by 42 publications

References 20 publications

Influence of cholesterol on the structure of stratum corneum lipid model membrane

Influence of cholesterol on the structure of stratum corneum lipid model membrane

Hydration properties of N-(α-hydroxyacyl)-sphingosine: X-ray powder diffraction and FT–Raman spectroscopic studies

Preferential arrangement of lipids in the long-periodicity phase of a stratum corneum matrix model

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