Polymorphism of bovine lipocalin-2 gene and its impact on milk production traits and mastitis in Holstein Friesian cattle

Pokorska, Joanna; Piestrzyńska-Kajtoch, Agata; Kułaj, Dominika; Ochrem, Andrzej; Radko, Anna

doi:10.1016/j.ejbt.2019.04.004

Cited by 7 publications

(7 citation statements)

References 22 publications

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“…During bacterial infection, neutrophils maintain iron homeostasis by releasing LCN2 and lactoferrin (LTF) to sequester free iron ( Ali et al, 2017 ) and protect the lung from oxidative stress induced by iron and HBA and HBB molecules ( Tubsuwan et al, 2011 ). Furthermore, LCN2 decreases iron availability to limit the growth of pathogenic bacteria ( Xiao et al, 2017 ; Pokorska et al, 2019 ). Pasteurella multocida express outer membrane protein receptors for iron-binding proteins, and the expression of these proteins increases during conditions of iron restriction ( Prado et al, 2005 ).…”

Section: Discussionmentioning

confidence: 99%

Whole-Blood Transcriptome Analysis of Feedlot Cattle With and Without Bovine Respiratory Disease

et al. 2021

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Bovine respiratory disease (BRD) is one of the main factors leading to morbidity and mortality in feedlot operations in North America. A complex of viral and bacterial pathogens can individually or collectively establish BRD in cattle, and to date, most disease characterization studies using transcriptomic techniques examine bronchoalveolar and transtracheal fluids, lymph node, and lung tissue as well as nasopharyngeal swabs, with limited studies investigating the whole-blood transcriptome. Here, we aimed to identify differentially expressed (DE) genes involved in the host immune response to BRD using whole blood and RNA sequencing. Samples were collected from heifers (average arrival weight = 215.0 ± 5.3 kg) with (n = 25) and without (n = 18) BRD at a commercial feedlot in Western Canada. RNAseq analysis showed a distinct whole-blood transcriptome profile between BRD and non-BRD heifers. Further examination of the DE genes revealed that those involved in the host inflammatory response and infectious disease pathways were enriched in the BRD animals, while gene networks associated with metabolism and cell growth and maintenance were downregulated. Overall, the transcriptome profile derived from whole blood provided evidence that a distinct antimicrobial peptide-driven host immune response was occurring in the animals with BRD. The blood transcriptome of the BRD animals shows similarities to the transcriptome profiles obtained from lung and bronchial lymph nodes in other studies. This suggests that the blood transcriptome is a potential diagnostic tool for the identification of biomarkers of BRD infection and can be measured in live animals and used to further understand infection and disease in cattle. It may also provide a useful tool to increase the understanding of the genes involved in establishing BRD in beef cattle and be used to investigate potential therapeutic applications.

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Section: Discussionmentioning

confidence: 99%

Whole-Blood Transcriptome Analysis of Feedlot Cattle With and Without Bovine Respiratory Disease

et al. 2021

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“…During inflammatory reactions, LCN2 binds to released iron ions which contain unpaired electrons, thus providing protection against free-radical reactions [26,27]. Analysis of polymorphisms of the bovine lipocalin-2 gene showed that this gene had a significant impact on milk production traits [10]. Our studies showed that an analysis of genotypes within the locus g.98793763G>C of the lipocalin-2 gene may provide valuable information to select cows according to greater antioxidant capacity of milk produced by them.…”

Section: Discussionmentioning

confidence: 99%

“…The genotyping was carried out using DNA obtained from somatic cells of milk and the DNA isolation method developed by Pokorska et al [11]. Subsequently, the PCR reaction was performed to replicate a 592-bp segment of the bovine lipocalin-2 gene (composed of a portion of the promoter sequence, exon 1, and a fragment of intron 1) using the primers suggested by Pokorska et al [10]. Once PCR products were purified enzymatically using EPPiC (A&A Biotechnology, Gdynia, Poland), they were processed by sequencing using a BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) in accordance with the procedure described by Pokorska et al [10].…”

Section: Methodsmentioning

confidence: 99%

“…Subsequently, the PCR reaction was performed to replicate a 592-bp segment of the bovine lipocalin-2 gene (composed of a portion of the promoter sequence, exon 1, and a fragment of intron 1) using the primers suggested by Pokorska et al [10]. Once PCR products were purified enzymatically using EPPiC (A&A Biotechnology, Gdynia, Poland), they were processed by sequencing using a BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems) in accordance with the procedure described by Pokorska et al [10]. All the results and SNP positions were analyzed using Variant Reporter Software 2 (Applied Biosystems) and BioEdit Sequence Alignment Editor [12], in reference to NC_037338.1, Bos taurus breed Hereford chromosome 11, ARS-UCD 1.2, whole genome shotgun sequence.…”

Section: Methodsmentioning

confidence: 99%

“…The bovine lipocalin-2 gene (Gene ID: 526639, NCBI, Bethesda, MD, USA) consists of 7 exons and 6 introns, within which multiple polymorphisms were identified, including, in particular, single nucleotide polymorphisms (SNPs). Some of these polymorphisms have been associated with production traits of Holstein-Friesian cattle [10]. To date, there have been no reports in the literature regarding the association of the LCN2 gene polymorphisms and the antioxidant activity of cows’ milk, and therefore the principal objective of this study was to analyze polymorphisms within a segment of the promoter sequence, exon 1 and a fragment of intron 1 of the lipocalin-2 gene, and to determine their impact on the antioxidant activity of milk from cows of the Holstein-Friesian breed, black-and-white variety.…”

Section: Introductionmentioning

confidence: 99%

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Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows

Pokorska

Kułaj

Piestrzyńska-Kajtoch

et al. 2019

Animals

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Simple SummaryRecently, an increased interest in high health-value food products rich in natural antioxidants has been observed. Milk and milk products are one of the richest sources of biologically active compounds with antioxidant properties in products of animal origin. Lipocalin-2 (LCN2) is a small secreted protein, which is involved in inflammatory processes. Due to the fact that LCN-2 can be produced by various types of cells in response to oxidative stress and LCN2 gene polymorphism has been associated with the somatic cells count in cow’s milk, the aim of this study was to validate the association of LCN2 polymorphism with antioxidant activity of milk from Holstein-Friesian cows. Four single nucleotide polymorphisms (SNPs) was identified, one of which g.98793763G>C was associated with higher antioxidant capacity in milk. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and somatic cell count (SCC) in milk.AbstractIn the recent years, antioxidant properties of food products have become an important aspect for consumers. Milk is a very good source of easily absorbable proteins and minerals, as well as a valuable source of antioxidants. Lipocalin-2 (LCN2), given that, inter alia, it is produced in large quantities by various types of cells in response to oxidative stress caused by physical or chemical factors, it can be considered a protein that determines the total antioxidant capacity of milk. The main objective of this study was to analyze polymorphisms within the lipocalin-2 gene and to determine their impact on antioxidant activity of milk from Holstein-Friesian cows. The genotyping was carried out by sequencing of PCR products. To determine the antioxidant activity of milk, the Trolox equivalent antioxidant capacity (TEAC) method was used. A total of four polymorphic sites were identified in the examined segment of the bovine lipocalin-2 gene. It was shown that cows of the CC genotype at the locus g.98793763G>C produced milk of significantly higher antioxidant capacity. The antioxidant capacity of milk also varied according to the age of cows, their daily milk yield, and SCC in milk.

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Relative quantitative proteomic profiling of bovine colostrum and transition milk at onset of lactation

Masterson,

O'Callaghan,

O'Donovan

et al. 2024

International Dairy Journal

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Polymorphism of bovine lipocalin-2 gene and its impact on milk production traits and mastitis in Holstein Friesian cattle

Cited by 7 publications

References 22 publications

Whole-Blood Transcriptome Analysis of Feedlot Cattle With and Without Bovine Respiratory Disease

Whole-Blood Transcriptome Analysis of Feedlot Cattle With and Without Bovine Respiratory Disease

Impact of Bovine Lipocalin-2 Gene on the Antioxidant Activity of Milk from Polish Holstein-Friesian Cows

Relative quantitative proteomic profiling of bovine colostrum and transition milk at onset of lactation

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