Polymorphism in two genes for B2 high sulfur proteins of wool

Rogers, Geraldine R.; Hickford, Jonathan G. H.; Bickerstaffe, R.

doi:10.1111/j.1365-2052.1994.tb00531.x

Cited by 65 publications

(66 citation statements)

References 24 publications

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“…Although single nucleotide substitutions were observed between the KAP1.1 alleles, the major difference are that the A allele encodes one more decapeptide repeat than the B allele and two repeats more than the C allele (Rogers et al, 1994a Gender was found to have a significant effect on wool yield in both sire lines. In SL1, 24 months of age rams had a mean yield of 76.9 ± 0.40% which was higher (P = 0.013) than the ewes which had a mean yield of 75.5 ± 0.40%.…”

Section: Resultsmentioning

confidence: 87%

“…Numerous studies have described variation within both the KAP and KRT genes, including the work of Rogers et al (1994a); Parsons et al (1994a;1996); McLaren et al (1997) and Beh et al (2001). This genetic diversity could impact on wool fibre structure and hence variation in the genes, and proteins derived from them could therefore underpin variation in wool traits.…”

Section: Introductionmentioning

confidence: 99%

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10.4314/sajas.v40i5.65343

2020

South African Journal of Animal Science

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________________________________________________________________________________ AbstractThis study aimed at identifying gene markers associated with wool quality traits in Merino and Merino Cross sheep using a candidate gene approach. Polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) analysis was used to identify sequence variation in the KAP1.3 and K33 genes, while agarose gel electrophoresis was used to detect length variation in the KAP1.1 gene. Two half-sib families (SL1 and SL2) were created for analysis and wool samples were collected from the mid-side region of the SL1 progeny at 12, 24 and 36 months of age, and of the SL2 progeny at 12 months of age. The association of alleles was analysed for each year of phenotypic data by an analysis of variance (ANOVA) tests, using SPSS® version 15. Analysis of each of KAP1.1, KAP1.3 and K33 genes revealed potential gene markers to select for animals with increased staple length, increased staple strength, higher yield, whiter and brighter wool. The results obtained are consistent with KAP1.1, KAP1.3 and KRT1.2 being clustered on one chromosome. Results also indicated that the keratin genes on chromosome 11 are recombining relatively frequently at recombination "hotspots". It appears as though genes coding for the KRTs and KAPs have the potential to impact on wool quality and could potentially be exploited in gene marker-assisted selection programmes in the wool industry for the rapid conversion of wool from one type to another ________________________________________________________________________________

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Section: Resultsmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

10.4314/sajas.v40i5.65343

2020

South African Journal of Animal Science

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“…While length variation in the sequences encoding the cysteine-rich repeat segments has been described in KAP AE AA AD DD AC CC AC AB genes, it has only been reported previously in one family (KAP1) in sheep in which decapeptide length variation also occurs [10] and two families (KAP1 and KAP4) in humans [11,12]. The detection of length variation in the ovine KAP5-4 gene suggests that variation in the number of repeats coding for cysteine-rich segments may be a structural hallmark of some of the KAP genes.…”

Section: Resultsmentioning

confidence: 95%

Analysis of variation in the ovine ultra‐high sulphur keratin‐associated protein KAP5‐4 gene using PCR‐SSCP technique

et al. 2010

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Keratin-associated proteins (KAPs) are one of the main structural components of the wool fibre. Variation in the KAP genes (KRTAPs) may affect the structure of KAPs and hence wool characteristics. In this study, we used PCR-SSCP to analyse ovine KRTAP5-4, a gene encoding a member of the KAP5 family. Five different PCR-SSCP patterns were detected in the 250 sheep that were analysed. Either one or a combination of two patterns was observed for each sheep, which was consistent with these sheep being either homozygous or heterozygous at this locus. DNA sequencing revealed that these patterns represent five different DNA sequences. One of the sequences was identical to a published ovine KRTAP5-4 sequence. The remaining four were unique, but shared a high homology with the published ovine KRTAP5-4 sequence, suggesting that these sequences represent allelic variants of KRTAP5-4. There were a total of six SNPs and one length polymorphism in the sequences. Of the five SNPs found in the coding region, four were non-synonymous SNPs and would result in amino acid changes. The length polymorphism would affect the cysteine content of the putative peptide and this along with the SNPs may have an impact on the structure of KAP5-4, and hence affect wool traits.

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“…KAP3.2 (together with KAP1.1, KAP1.3 and K33) have been mapped to ovine chromosome 1 (Figure 3.5). Variations in all of the three genes (KAP1.1, KAP1.3 and K33) have been previously associated with variation in wool traits (Itenge et al, 2009;Itenge et al, 2010;Rogers et al, 1994b). It is therefore suggested that sires that are heterozygous get investigated in further studies.…”

Section: Discussionmentioning

confidence: 99%

“…There are some reports associating variation in the KRT and KAP genes with variation in wool traits. Parsons et al (1994b) and Beh et al (2001) reported associations between variation in KAPs and mean fibre diameter in Merino sheep, while Rogers et al (1994b) reported association between staple strength in Romney sheep and the region spanning the KAP1.1/KAP1.3/K33 loci on ovine chromosome 11. Itenge et al (2009;2010) reported association between variation in the KAP1.1 gene with variation in yield.…”

Section: Previously Published Association Of Genetic Markers With Woomentioning

confidence: 99%

Identification of Polymorphism in the Keratin Genes (KAP3.2, KAP6.1, KAP7, KAP8) and Microsatellite BfMS in Merino Sheep Using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) Analysi

Itenge¹

2012

Electrophoresis

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Polymorphism in two genes for B2 high sulfur proteins of wool

Cited by 65 publications

References 24 publications

10.4314/sajas.v40i5.65343

10.4314/sajas.v40i5.65343

Analysis of variation in the ovine ultra‐high sulphur keratin‐associated protein KAP5‐4 gene using PCR‐SSCP technique

Identification of Polymorphism in the Keratin Genes (KAP3.2, KAP6.1, KAP7, KAP8) and Microsatellite BfMS in Merino Sheep Using Polymerase Chain Reaction-Single Strand Conformational Polymorphism (PCR-SSCP) Analysi

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