Polymorphism in soft coral larvae revealed by amplified fragment-length polymorphism (AFLP) markers

Barki, Y.; Douek, Jacob; Graur, Dan; Gateño, D.; Rinkevich, Baruch

doi:10.1007/s002270050005

Cited by 19 publications

(8 citation statements)

References 22 publications

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“…gibbosa ranged between 48.4% and 78.7%. These results are consistent with AFLP data reported for other European invertebrates such as the soft coral Parerythropodium fulvum fulvum (Barki et al . 2000), the sea anemone Actinia equina (Douek et al .…”

Section: Discussionsupporting

confidence: 93%

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Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

2005

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In this study, the population structure of the Lusitanian sea star Asterina gibbosa was assessed using amplified fragment length polymorphism (AFLP). One hundred and twenty-two AFLP loci were analysed in 159 individuals from eight populations from across the species' range and revealed high levels of genetic diversity, with all individuals but two harbouring a unique banding pattern. As reported for other marine invertebrates, we found high levels of genetic differentiation between the Atlantic and Mediterranean basins, suggesting that the Strait of Gibraltar represents a major barrier to dispersal for this sea star. Our assignment studies suggest that, in the Atlantic, a measurable degree of gene flow occurs between populations, which could result in the isolation-by-distance pattern of differentiation observed in this basin. In contrast, no evidence of contemporary gene flow was found in the Mediterranean, suggesting contrasting patterns of dispersal of Asterina gibbosa in the Atlantic and Mediterranean basins.

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Section: Discussionsupporting

confidence: 93%

“…2004) including marine invertebrates (Triantaphyllidis et al . 1997; Fetzner & Crandall 1999; Barki et al . 2000; Wilding et al .…”

Section: Introductionmentioning

confidence: 99%

Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

2005

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“…1992; Edmands & Potts 1997; amplified fragment length polymorphisms (AFLPs), Lopez et al . (1999); Barki et al . 2000; D. Brazeau, pers.…”

Section: Intraspecific Mtdna Divergence In the Anthozoamentioning

confidence: 99%

Slow mitochondrial DNA sequence evolution in the Anthozoa (Cnidaria)

et al. 2002

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Mitochondrial genes have been used extensively in population genetic and phylogeographical analyses, in part due to a high rate of nucleotide substitution in animal mitochondrial DNA (mtDNA). Nucleotide sequences of anthozoan mitochondrial genes, however, are virtually invariant among conspecifics, even at third codon positions of protein-coding sequences. Hence, mtDNA markers are of limited use for population-level studies in these organisms. Mitochondrial gene sequence divergence among anthozoan species is also low relative to that exhibited in other animals, although higher level relationships can be resolved with these markers. Substitution rates in anthozoan nuclear genes are much higher than in mitochondrial genes, whereas nuclear genes in other metazoans usually evolve more slowly than, or similar to, mitochondrial genes. Although several mechanisms accounting for a slow rate of sequence evolution have been proposed, there is not yet a definitive explanation for this observation. Slow evolution and unique characteristics may be common in primitive metazoans, suggesting that patterns of mtDNA evolution in these organisms differ from that in other animal systems.

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“…Microsatellites, possibly the best molecular candidates for molecular markers, are timeconsuming, costly to develop (Goldstein and Pollock 1997) and, in many coral species, are arduously generated (Marquez et al 2003). Another molecular marker is the AFLP methodology, which has been gaining popularity lately (Barki et al 2000;Bonin et al 2007). Applying this methodology in hermatypic corals is impaired by the coexistence of algal cells, resulting in algal-contaminated AFLP bands.…”

Section: Introductionmentioning

confidence: 99%

Maternal-larval population genetic traits in Stylophora pistillata, a hermaphroditic brooding coral species

2011

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Aspects of maternal-planula larval genetics in the monoecious scleractinian coral Stylophora pistillata (Red Sea, Eilat) were studied by amplified fragment length polymorphism (AFLP) methodology in two successive reproductive seasons. In total, 293 planulae and 10 adult colonies were analyzed. In June 2006, 147 planulae were collected from 10 shallow water colonies. In March, April and June 2007, 146 additional planulae were sampled from five of the ten 2006 sampled colonies. All AFLP products showed unalike band profiles indicating a fully sexual production pattern. We used 181 and 210 putative AFLP loci, of which the overall level of polymorphism in 2006 was 92 and 99 % in 2007 (respectively). Differences were also observed between 2006 and 2007 reproductive seasons in terms of total average gene diversity (0.191 vs. 0.247, respectively), suggesting fast turnover of sperm donor genotypes. In addition, increased numbers of potential sperm donor colonies in the vicinity of gravid females showed no impact on genetic differentiation levels in released larvae. UPGMA tree revealed clustering of maternal genotypes and their offspring, suggesting, as expected, high relatedness between planulae and their mothers. In addition, the average heterozygosity of each group of siblings was persistently lower than heterozygosity calculated for the respective maternal colony, suggesting the possibility of partial inbreeding. This trend of reduced genetic heterogeneity in Stylophora pistillata is an alarming sign for populations residing in the northern Red Sea coral reefs.

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Polymorphism in soft coral larvae revealed by amplified fragment-length polymorphism (AFLP) markers

Cited by 19 publications

References 22 publications

Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

Gene‐flow patterns in Atlantic and Mediterranean populations of the Lusitanian sea star Asterina gibbosa

Slow mitochondrial DNA sequence evolution in the Anthozoa (Cnidaria)

Maternal-larval population genetic traits in Stylophora pistillata, a hermaphroditic brooding coral species

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