Polymorphism in Litopenaeus vannamei genes and cross-species amplification in other shrimp species

Abstract

The objective of this work was to assess polymorphisms within Litopenaeus vannamei candidate genes related to growth performance, and a possible association between weight genotypes and phenotypes in two shrimp closed lines. Three EST-SSR (serine-arginine, 60S ribosomal, and troponin) and one EST-SNP (crustacyanin) were selected and evaluated in L. vannamei and seven other marine shrimp species, besides two freshwater species. Genotypes had no effect on total body weight. Although no association was observed in the sampled population, these markers showed amplification for most of the evaluated species and, therefore, may be useful in further genetic studies on shrimp species.

Molecular markers for differentiation of Fenneropenaeus merguiensis among Penaeus species

“…In addition, three microsatellites of expressed sequence tags (serine‐arginine, troponin and the 60S ribosomal subunit) were used to differentiate P. vannamei from other shrimp species. The success rates of cross‐species amplification using SSR for serine‐arginine, troponin and the 60S ribosomal subunit were 89%, 55% and 89% respectively (Santos et al, 2018).…”

Characterization of microsatellite markers in the coding regions of the Penaeus vannamei genome

“…In this context, the study and identification of microsatellite markers, also known as Short Tandem Repeats (STRs), hold great potential for practical applications. These markers offer a cost-effective means to monitor individual genetic characteristics [ 6 , 7 ]. Microsatellites possess several advantageous features, including broad genome coverage, multi-allelic nature, co-dominance, and polymorphism, making them excellent tools for studying genetic variation and constructing genetic maps [ 8 , 9 ].…”