Polymorphism in a sex‐linked <scp>DNA</scp> marker located on <scp>LG23</scp> in Hainan strain of Nile tilapia (<scp><i>Oreochromis niloticus</i></scp>)

Jiang, Dongneng; Kuang, Zi-Ying; Yang, Kong-Song; Huang, Yuanqing; Mustapha, Umar Farouk; Guo, Xiang-Zhao; Zheng, De-Feng; Dong, Zhongdian; Shi, Hongjuan

doi:10.1111/jwas.12815

Cited by 3 publications

(1 citation statement)

References 31 publications

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“…XX gsdf -/- and gsdf +/+ progenies were obtained by crossing an XX gsdf +/- female with XY gsdf +/- male fish. Genotyping using a sex-linked marker (Marker-5) determined the genetic sex of each fish ( 31 , 32 ). The gsdf mutant fish were detected using the Bst NI enzyme, polyacrylamide gels electrophoresis (PAGE), or Sanger sequencing ( Figure S1 ).…”

Section: Methodsmentioning

confidence: 99%

Homozygous Mutation of gsdf Causes Infertility in Female Nile Tilapia (Oreochromis niloticus)

et al. 2022

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Gonadal somatic cell-derived factor (Gsdf) is a member of the TGF-β superfamily, which exists mainly in fishes. Homozygous gsdf mutations in Japanese medaka and zebrafish resulted in infertile females, and the reasons for their infertility remain unknown. This study presents functional studies of Gsdf in ovary development using CRISPR/Cas9 in Nile tilapia (Oreochromis niloticus). The XX wild type (WT) female fish regularly reproduced from 12 months after hatching (mah), while the XX gsdf-/- female fish never reproduced and were infertile. Histological observation showed that at 24 mah, number of phase IV oocyte in the XX gsdf-/- female fish was significantly lower than that of the WT fish, although their gonadosomatic index (GSI) was similar. However, the GSI of the XX gsdf-/- female at 6 mah was higher than that of the WT. The mutated ovaries were hyperplastic with more phase I oocytes. Transcriptome analysis identified 344 and 51 up- and down-regulated genes in mutants compared with the WT ovaries at 6 mah. Some TGF-β signaling genes that are critical for ovary development in fish were differentially expressed. Genes such as amh and amhr2 were up-regulated, while inhbb and acvr2a were down-regulated in mutant ovaries. The cyp19a1a, the key gene for estrogen synthesis, was not differentially expressed. Moreover, the serum 17β-estradiol (E2) concentrations between XX gsdf-/- and WT were similar at 6 and 24 mah. Results from real-time PCR and immunofluorescence experiments were similar and validated the transcriptome data. Furthermore, Yeast-two-hybrid assays showed that Gsdf interacts with TGF-β type II receptors (Amhr2 and Bmpr2a). Altogether, these results suggest that Gsdf functions together with TGF-β signaling pathway to control ovary development and fertility. This study contributes to knowledge on the function of Gsdf in fish oogenesis.

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Section: Methodsmentioning

confidence: 99%

Homozygous Mutation of gsdf Causes Infertility in Female Nile Tilapia (Oreochromis niloticus)

et al. 2022

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Fish Selection Based on DNA Markers: Literature Review

Harahap

Haser

Febri

et al. 2022

JISA

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Selection of fish based on DNA markers is a method or technique that has started to develop rapidly in the field of genetics and fish breeding. Selection based on DNA markers utilizes the genetic information contained in fish DNA to obtain individuals with characteristics appropriate to the stages and production of aquaculture in a timely, efficient, and measurable manner. This literature review presents several discussions and literature sources that are quite relevant regarding various aspects of DNA marker-based fish selection, including the basic principles, analytical methods, benefits, and challenges based on DNA markers, and discusses how the most recent developments in the use of methods in the process of genetic breeding and fish selection, as well as the potential for future applications.

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Dnmt3aa but Not Dnmt3ab Is Required for Maintenance of Gametogenesis in Nile Tilapia (Oreochromis niloticus)

Wang

Qin

et al. 2021

IJMS

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Dnmt3a, a de novo methyltransferase, is essential for mammalian germ line DNA methylation. Only one Dnmt3a is identified in mammals, and homozygous mutants of Dnmt3a are lethal, while two Dnmt3a paralogs, dnmt3aa and dnmt3ab, are identified in teleosts due to the third round of genome duplication, and homozygous mutants of dnmt3aa and dnmt3ab are viable in zebrafish. The expression patterns and roles of dnmt3aa and dnmt3ab in gonadal development remain poorly understood in teleosts. In this study, we elucidated the precise expression patterns of dnmt3aa and dnmt3ab in tilapia gonads. Dnmt3aa was highly expressed in oogonia, phase I and II oocytes and granulosa cells in ovaries and spermatogonia and spermatocytes in testes, while dnmt3ab was mainly expressed in ovarian granulosa cells and testicular spermatocytes. The mutation of dnmt3aa and dnmt3ab was achieved by CRISPR/Cas9 in tilapia. Lower gonadosomatic index (GSI), increased apoptosis of oocytes and spermatocytes and significantly reduced sperm quality were observed in dnmt3aa−/− mutants, while normal gonadal development was observed in dnmt3ab−/− mutants. Consistently, the expression of apoptotic genes was significantly increased in dnmt3aa−/− mutants. In addition, the 5-methylcytosine (5-mC) level in dnmt3aa−/− gonads was decreased significantly, compared with that of dnmt3ab−/− and wild type (WT) gonads. Taken together, our results suggest that dnmt3aa, not dnmt3ab, plays important roles in maintaining gametogenesis in teleosts.

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Polymorphism in a sex‐linked DNA marker located on LG23 in Hainan strain of Nile tilapia (Oreochromis niloticus)

Cited by 3 publications

References 31 publications

Homozygous Mutation of gsdf Causes Infertility in Female Nile Tilapia (Oreochromis niloticus)

Homozygous Mutation of gsdf Causes Infertility in Female Nile Tilapia (Oreochromis niloticus)

Fish Selection Based on DNA Markers: Literature Review

Dnmt3aa but Not Dnmt3ab Is Required for Maintenance of Gametogenesis in Nile Tilapia (Oreochromis niloticus)

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