Dnmt3aa but Not Dnmt3ab Is Required for Maintenance of Gametogenesis in Nile Tilapia (Oreochromis niloticus)

Wang, Feilong; Qin, Zuliang; Li, Zhiqiang; Yang, Shuangyi; Gao, Tian; Sun, Lina; Wang, Deshou

doi:10.3390/ijms221810170

Cited by 10 publications

(5 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescence signals were captured by confocal microscopy (Olympus FV3000) (Olympus, Tokyo, Japan). The probes and qPCR primer sequences [ 38 , 39 ] used in this study are listed in Table S2 .…”

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the BRD Family in Nile Tilapia (Oreochromis niloticus)

Zhang

et al. 2022

Animals

Self Cite

View full text Add to dashboard Cite

The bromodomain (BRD) proteins specifically recognize the N-acetyllysine motifs, which is a key event in the reading process of epigenetic marks. BRDs are evolutionarily highly conserved. Over recent years, BRDs attracted great interest because of their important roles in biological processes. However, the genome-wide identification of this family was not carried out in many animal groups, in particular, in teleosts. Moreover, the expression patterns were not reported for any of the members in this family, and the role of the BRD family was not extensively studied in fish reproduction. In this study, we identified 16 to 120 BRD genes in 24 representative species. BRDs expanded significantly in vertebrates. Phylogenetic analysis showed that the BRD family was divided into eight subfamilies (I–VIII). Transcriptome analysis showed that BRDs in Nile tilapia (Oreochromis niloticus) exhibited different expression patterns in different tissues, suggesting that these genes may play different roles in growth and development. Gonadal transcriptome analysis showed that most of the BRDs display sexually dimorphic expression in the gonads at 90 and 180 dah (days after hatching), including 21 testis-dominated genes (brdt, brd4a and brd2b, etc.), and nine ovary-dominated genes (brd3b, brd2a and kat2a, etc.). Consistent with transcriptomic data, the results of qRT-PCR and fluorescence in situ hybridization showed that brdt expression was higher in the testis than in the ovary, suggesting its critical role in the spermatogenesis of the tilapia. Male fish treated with JQ1 (BET subfamily inhibitor) displayed abnormal spermatogenesis. The numbers of germ cells were reduced, and the expression of steroidogenic enzyme genes was downregulated, while the expression of apoptosis-promoting genes was elevated in the testis tissue of treated fish. Our data provide insights into the evolution and expression of BRD genes, which is helpful for understanding their critical roles in sex differentiation and gonadal development in teleosts.

show abstract

Section: Methodsmentioning

confidence: 99%

Genome-Wide Identification and Characterization of the BRD Family in Nile Tilapia (Oreochromis niloticus)

Zhang

et al. 2022

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

“…Fluorescence in situ hybridization (FISH) experiment was performed as previously described [28]. The medaka smo genespecific primers for FISH are listed in Supplemental Table S1.…”

Section: Fluorescence In Situ Hybridization and Immunofluorescencementioning

confidence: 99%

Smoothened mediates medaka spermatogonia proliferation via Gli1–Rgcc–Cdk1 axis

Zhao

Liu

et al. 2023

Biology of Reproduction

View full text Add to dashboard Cite

The proliferation of spermatogonia directly affect spermatogenesis and male fertility, but its underlying molecular mechanisms are poorly understood. In this study, Smoothened (Smo), the central transducer of Hedgehog signaling pathway, was characterized in medaka (Oryzias latipes), and its role and underlying mechanisms in the proliferation of spermatogonia were investigated. Smo was highly expressed in spermatogonia. In ex vivo testicular organ culture and a spermatogonial cell line (SG3) derived from medaka mature testis, Smo activation promoted spermatogonia proliferation, while its inhibition induced apoptosis. The expression of glioma-associated oncogene homolog 1 (gli1) and regulator of cell cycle (rgcc) was significantly upregulated in SG3 after Smo activation. Furthermore, Gli1 transcriptionally upregulated the expression of rgcc, and Rgcc overexpression rescued cell apoptosis caused by Smo or Gli1 inhibition. Co-immunoprecipitation assay indicated that Rgcc could interact with cyclin dependent kinase 1 (Cdk1) to regulate the cell cycle of spermatogonia. Collectively, our study firstly reveals that Smo mediates the proliferation of spermatogonia through Gli1-Rgcc-Cdk1 axis. In addition, Smo and Gli1 are necessary of the survival of spermatogonia. This study deepens our understanding of spermatogonia proliferation and survival at the molecular level, and provides insights into male fertility control and reproductive diseases treatment.

show abstract

“…Recently, studies have focused on better determining which genes have an influence on gametogenesis 151–153 . Several influential genes regulating sex include, but are not limited to, double sex and mab‐3 related transcription factor 1 ( Dmrt1 ), 154 forkhead box transcription factor L2 ( Foxl2 ) 155 and R‐spondin‐1 ( Rspo1 ) 156 .…”

Section: Traits To Optimisementioning

confidence: 99%

Optimising commercial traits through gene editing in aquaculture: Strategies for accelerating genetic improvement

Moran,

Jones,

Jensen

et al. 2023

Reviews in Aquaculture

View full text Add to dashboard Cite

Aquaculture is one of the fastest‐growing food production sectors. As the global human population continues to increase and further pressure is added to the prospects of achieving global food security, aquaculture is expected to play an integral role in meeting future nutrition demands. With advances in genetic technologies over recent years, much progress has been made within the realm of selective breeding. Despite success, selective breeding programs have limitations to the rate of genetic gain they can achieve. The incorporation of targeted genetic technologies, such as gene editing, into research related to selective breeding programs will help identify specific genes related to commercially desirable traits, as well as expedite genetic improvement. This review summarises research encompassing the most commonly targeted traits using gene editing within aquaculture, namely reproduction and development, pigmentation, growth and disease resistance. In addition, this review illustrates how the incorporation of gene editing can expedite genetic improvement through the rapid fixation of desirable alleles, as well as suggests strategies to accelerate genetic improvement for aquaculture production.

show abstract

Dnmt3aa but Not Dnmt3ab Is Required for Maintenance of Gametogenesis in Nile Tilapia (Oreochromis niloticus)

Cited by 10 publications

References 77 publications

Genome-Wide Identification and Characterization of the BRD Family in Nile Tilapia (Oreochromis niloticus)

Genome-Wide Identification and Characterization of the BRD Family in Nile Tilapia (Oreochromis niloticus)

Smoothened mediates medaka spermatogonia proliferation via Gli1–Rgcc–Cdk1 axis

Optimising commercial traits through gene editing in aquaculture: Strategies for accelerating genetic improvement

Contact Info

Product

Resources

About