Polymorphism analysis of csd gene in six Apis mellifera subspecies

Wang, Zilong; Li, Yong; Wu, Xiaobo; Wei, Yan; Zeng, Zhen

doi:10.1007/s11033-011-1069-7

Cited by 18 publications

(30 citation statements)

References 20 publications

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“…Different alleles of csd are very common, this being a highly diverse and, presumably, fast evolving locus (Beye et al 2003;Hasselmann and Beye 2004;Hasselmann et al 2008b;Wang et al 2012). This diversity in csd alleles means that, in normal populations, brood viability remains high.…”

Section: Introductionmentioning

confidence: 99%

Genetic tests for alleles of complementary-sex-determiner to support honeybee breeding programmes

Hyink

Laas²,

Dearden

2012

Apidologie

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The honeybee haplodiploid sex determination system depends on genetic variation at the complementary sex-determiner (csd) locus. In closed populations of honeybees, especially those undergoing selective breeding, the number of csd alleles can drop such that brood viability is affected. Here we present two polymerase chain reaction tests that allow the discrimination of csd alleles. Such tests should find utility in bee breeding programmes allowing the tracking and maintenance of csd alleles through successive generations.genetic test / honeybee breeding / sex determination / high-resolution melting

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Section: Introductionmentioning

confidence: 99%

Genetic tests for alleles of complementary-sex-determiner to support honeybee breeding programmes

Hyink

Laas²,

Dearden

2012

Apidologie

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“…Sequencing the csd alleles gives opportunity to select the most proper queen for insemination. Until recently, alleles have mainly been genotyped by Sanger sequencing [16,21] or, most recently, by an HRM-based technique [11]. However, csd allele typing directly from the queen, which would be able to provide sequence information, was lacking prior to this study.…”

Section: A High-throughput Sequencing Technique Is Provided For Detecmentioning

confidence: 99%

“…The genes of honey bees tend to show high levels of polymorphism [16,20]. According to Wang, et al [21], A. mellifera subspecies A. m. carnica and A. m. ligustica also show a high level of polymorphism in csd genes.…”

Section: Introductionmentioning

confidence: 99%

An Alternative, High Throughput Method to Identify Csd Alleles of the Honey Bee

Kolics

Parrag

Házi³

et al. 2020

Insects

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Applying instrumental insemination in closely related honey bee colonies often leads to frequent lethality of offspring causing colony collapse. This is due to the peculiarities of honey bee reproductive biology, where the complementary sex determination (csd) gene drives sex determination within a haplodiploid system. Diploid drones containing homozygous genotypes are lethal. Tracking of csd alleles using molecular markers prevents this unwanted event in closed breeding programs. Our approach described here is based on high throughput sequencing (HTS) that provides more data than traditional molecular techniques and is capable of analysing sources containing multiple alleles, including diploid individuals as the bee queen. The approach combines HTS technique and clipping wings as a minimally invasive method to detect the complementary sex determiner (csd) alleles directly from honey bee queens. Furthermore, it might also be suitable for screening alleles of honey harvested from hives of a closed breeding facility. Data on alleles of the csd gene from different honey bee subspecies are provided. It might contribute to future databases that could potentially be used to track the origin of honey. With the help of tracking csd alleles, more focused crossings will be possible, which could in turn accelerate honey bee breeding programmes targeting increase tolerance against varroosis as well.

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“…So far, a csd gene has been sequenced only in species of bees of the genus Apis, and it is highly polymorphic, even within subspecies (Wang et al 2012). It is located adjacent to the more conserved feminizer (fem) (Hasselmann et al 2008), and we therefore started with localizing feminizer in the genome.…”

Section: Identification Of a Putative Feminizer Ortholog And Duplicatmentioning

confidence: 99%

Braconidae revisited:Bracon brevicornisgenome showcases the potential of linked-read sequencing in identifying a putativecomplementary sex determinergene

Ferguson

Pannebakker

Centurión

et al. 2020

Preprint

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Bracon brevicornis is an ectoparasitoid of a wide range of larval-stage Lepidopterans, including several pests of important crops, such as the corn borer, Ostrinia nubilalis. It is also one of the earliest documented cases of complementary sex determination in Hymenoptera. Here, we present the linked-read genome of B. brevicornis, complete with an ab initio-derived annotation and protein comparisons with fellow braconids, Fopius arisanus and Diachasma alloem. We demonstrate the potential of linked-read assemblies in exploring regions of heterozygosity and search for structural and homology-derived evidence of the complementary sex determiner gene (csd).

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Polymorphism analysis of csd gene in six Apis mellifera subspecies

Cited by 18 publications

References 20 publications

Genetic tests for alleles of complementary-sex-determiner to support honeybee breeding programmes

Genetic tests for alleles of complementary-sex-determiner to support honeybee breeding programmes

An Alternative, High Throughput Method to Identify Csd Alleles of the Honey Bee

Braconidae revisited:Bracon brevicornisgenome showcases the potential of linked-read sequencing in identifying a putativecomplementary sex determinergene

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