Polymorphic assembly of virus-capsid proteins around DNA and the cellular uptake of the resulting particles

Ruiter, Mark V. de; Hee, R. M. van der; Driessen, A.J.M.; Keurhorst, E.D.; Hamid, Mazin K.; Cornelissen, Jeroen J. L. M.

doi:10.1016/j.jconrel.2019.06.019

Cited by 40 publications

(49 citation statements)

References 45 publications

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“…Reassembly can also be triggered by mixing the cargo with coat proteins above the critical assembly concentration of the VLPs in a similar fashion to the formation of micelles [14]. The successful encapsulation of cargo depends on the size and surface charge of the cargo, electrostatic interactions, hydrophobicity/hydrophilicity, and other unique binding interactions that occur during nanoassembly [15][16][17]. In some cases, the reassembly is based on electrostatic interactions between the positively charged interior surfaces of the capsid proteins and a negatively charged cargo that mimics the negatively charged nucleic acids [18].…”

Section: Encapsulation and Conjugation Techniques For Vlpsmentioning

confidence: 99%

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Chung

Cai

Steinmetz

2020

Advanced Drug Delivery Reviews

283

241

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Viral nanoparticles (VNPs) encompass a diverse array of naturally occurring nanomaterials derived from plant viruses, bacteriophages, and mammalian viruses. The application and development of VNPs and their genomefree versions, the virus-like particles (VLPs), for nanomedicine is a rapidly growing. VLPs can encapsulate a wide range of active ingredients as well as be genetically or chemically conjugated to targeting ligands to achieve tissue specificity. VLPs are manufactured through scalable fermentation or molecular farming, and the materials are biocompatible and biodegradable. These properties have led to a wide range of applications, including cancer therapies, immunotherapies, vaccines, antimicrobial therapies, cardiovascular therapies, gene therapies, as well as imaging and theranostics. The use of VLPs as drug delivery agents is evolving, and sufficient research must continuously be undertaken to translate these therapies to the clinic. This review highlights some of the novel research efforts currently underway in the VNP drug delivery field in achieving this greater goal.

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Section: Encapsulation and Conjugation Techniques For Vlpsmentioning

confidence: 99%

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Chung

Cai

Steinmetz

2020

Advanced Drug Delivery Reviews

283

241

View full text Add to dashboard Cite

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“…VLPs display disassembly–reassembly behavior and morphological changes with changes in ionic strength caused by shifts in pH, temperature, or protein–protein and protein–RNA interactions. [ 29,133,134 ] VLPs can be engineered with chemical and/or genetic modification to carry genes or drugs or for use as vaccines. [ 73,130 ] Linking drugs to VLPs increases both their stability and cytotoxicity of the drug.…”

Section: Biomedical Applications Of Vlpsmentioning

confidence: 99%

“…[ 27 ] However, polymer‐based NPs are limited by their structural heterogeneity, particle instability, slow drug release, and potential immunogenicity. [ 28,29 ] Liposome NPs are limited by their particle instability, rapid clearance, and autogenous membrane fusion with off‐target cells. [ 7,30,31 ] Metal‐based NPs are stable, but lack specificity and are highly toxic.…”

Section: Introductionmentioning

confidence: 99%

Virus‐Like Particles as Theranostic Platforms

Sun

Cui

2020

Advanced Therapeutics

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In recent years, theranostics have received enormous attention for their use in individualized diagnosis and treatment. The ability of viral coat protein subunits to assemble hierarchically can be used to package various kinds of foreign compounds. Moreover, numerous chemistries and modification strategies allow the functionalization of these virus-like particles (VLPs) with imaging reagents, targeting ligands, or therapeutic molecules. VLP nanoplatforms have great potential utility in the design of sophisticated multifunctional theranostic platforms. Numerous studies have reported the construction of multifunctional nanoparticles using VLPs for targeted diagnoses and treatment. In this paper, the latest progress in molecular imaging, drug delivery, and multifunctional theranostic nanodevices based on VLPs is reviewed.

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“…With a better understanding of the electrostatic interaction induced assembly of VLPs, attempts at constructing various shaped VLPs and encapsulating functional cargos were carried out. 40,43,46,52,53 Sinn et al 46 managed to utilize assembly of water-soluble Pt(Ⅱ) amphiphiles to template CCMV VLPs assembly. This formation of VLPs can be tailored by modulating the supramolecular interactions between negatively charged template and capsid proteins (Figure 2.3).…”

Section: Electrostatic Interaction Induced Assemblymentioning

confidence: 99%

“…Electrostatic interaction induced assembly of protein cages into hierarchical structure is of great interest. There are three main advantages of applying electrostatic interaction: a) most natural protein cages carry either negative or positive surface charge near neutral pH, taking TMV 84 and CCMV 53 as example, they are negatively charged on their outer surface, and the charges on the surface are distributed evenly due to the intrinsic symmetrical structure of these protein cages; b) electrostatic interaction can be controlled through pH and salt concentration; c) both synthetic and natural components can be introduced as the contrary building blocks to provide vary functions.…”

Section: Hierarchical Lattices Of Protein Cages Via Electrostatic Intmentioning

confidence: 99%

Controlling the Assembly of Protein Cages towards Functional Supramolecular Materials

Cao¹

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In natural as well as synthetic systems, supramolecular interactions plays a vital role in essential life processes, such as catalysis, 1 protein synthesis, 2 cell replication and differentiation, 3 as well as molecule transport and delivery. 4 These supramolecular interactions are reversible, stimuli responsive and dynamic. Understanding and modulating these interactions would thus offer an effective manner to control the organization of a range of complexes which would benefit their potential properties and applications. Protein cages such as viruses and bacterial nano-compartment are perfect examples of natural assemblies formed by such supramolecular interactions. 5-8 Recent years have witnessed an increasing attempt to understand the assembly process of virus-like particles, in that way using these capsid proteins to construct functional materials for various applications. 9, 10 More specifically, plant viruses, as an example, consist of capsid proteins which envelop their genome inside highly symmetric cages via both electrostatic interactions and hydrophobic interaction. Furthermore, these viruses are uniform in size and hollow, which allows the encapsulation of functional cargos. These hollow nanometer-sized protein structures provide a template for the study of host-guest interactions in confined space, which in theory should be different from bulk solution, in addition, the specific interactions parameters could also provide vital information of the physical microenvironment inside protein cages. The work described in this thesis combined supramolecular chemistry and physical virology, by studying host-guest interactions inside the cowpea chlorotic mottle virus (CCMV) capsid, and ultimately utilize these interactions to induce the self-assembly of virus-based protein into structures with control in multi dimension. Chapter 2 provides an overview of recently reported assembly of virus-like particles induced by supramolecular interactions, that have been used as nanocontainers, nanoreactors and nano-templates for inorganic or organic material synthesis. Furthermore, the assembly of protein cages via supramolecular interactions into highly ordered 2D and 3D superstructures is described.

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Polymorphic assembly of virus-capsid proteins around DNA and the cellular uptake of the resulting particles

Cited by 40 publications

References 45 publications

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Viral nanoparticles for drug delivery, imaging, immunotherapy, and theranostic applications

Virus‐Like Particles as Theranostic Platforms

Controlling the Assembly of Protein Cages towards Functional Supramolecular Materials

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