Polymethacrylate monolithic and hybrid particle-monolithic columns for reversed-phase and hydrophilic interaction capillary liquid chromatography

Jandera, Pavel; Urban, Jiřı́; Škeříková, Veronika; Langmaier, Pavel; Kubíčková, Romana; Planeta, Josef

doi:10.1016/j.chroma.2009.09.041

Cited by 57 publications

(33 citation statements)

References 27 publications

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“…Each variation of the polymerization condition has significant effect on the structure of monolith. In fact, minor changes in the composition of the polymer mixture can affect the performance of monolithic column [23,35]. Hence, it is important to obtain the optimized polymerization condition.…”

Section: Results and Discussion Preparation Of Monolith Column Poly-(mmentioning

confidence: 99%

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

Malis

Tasfiyati

Iftitah

et al. 2015

J. Pure App. Chem. Res.

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A polymer monolithic column, poly-(methacrylatoethyltrimethylammonium chloride-covinylbenzyl chloride-co-ethylene dimethacrylate) or poly-(MATE-co-VBC-co-EDMA) was successfully prepared in the current study by one-step thermally initiated in situ polymerization, confined in a steel tubing of 0.5 mm i.d. and 1/16" o.d. The monoliths were prepared from methacrylatoethyltrimethylammonium chloride (MATE) and vinylbenzyl chloride (VBC) as monomer and ethylene dimethacrylate (EDMA) as crosslinker using a binary porogen system of 1-propanol and 1,4-butanediol. The inner wall of steel tubing was pretreated with 3-methacryloxypropyl-trimethoxysilane (MAPS). In order to obtain monolith with adequate column efficiency and low flow resistance, some parameters such as total monomer concentration (%T) and crosslinker concentration (%C) were optimized. The morphology of this monolith was assessed by scanning electron microscopy (SEM). The properties of the monolithic column, such as permeability, binding capacity, and pore size distribution were also characterized in detail. From the results of the characterization of all monolith variation, monolith with %T 30 %C 50 and %T 35 %C 50 give the best characteristic. These monoliths have high permeability, adequate molecular recognition sites (represented with binding capacity value of over 20 mg/mL), and have over 80% flow through pores in their pore structure contribute to low flow resistance. The resulted monolithic columns have promising potential for dual mode liquid chromatography. MATE may contribute for anion-exchange while VBC may responsible for reversed-phase liquid chromatography.

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Section: Results and Discussion Preparation Of Monolith Column Poly-(mmentioning

confidence: 99%

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

Malis

Tasfiyati

Iftitah

et al. 2015

J. Pure App. Chem. Res.

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“…Columns providing a dual retention mechanism offer the possibility of selecting between the two retention modes with different separation selectivity on a single column, just by changing the composition of the mobile phase, which is advantageous for separations of sample containing compounds with significant differences in polarities. For the separation of phenolic acids, hybrid particle-monolithic sulfobetaine columns were applied [39]. The hybrid columns showed reduced porosity and hold-up volumes, approximately 2-2.5 times lower in comparison to the pure monolithic columns prepared in the whole volume of empty capillaries.…”

Section: Phenolic Acidsmentioning

confidence: 99%

“…7. U-shape plots of the retention factors of phenolic acids, k, versus the volume fraction of buffer, ϕ [39] Some phenolic acids were investigated on different columns: silica, amide, diol and cyanophenyl [40]. Two different mobile phases: 90:10 MeCN/H 2 O with ammonium formate and formic acid, or 90:10 MeCN/H 2 O with ammonium acetate and ammonium hydroxide were applied.…”

Section: Phenolic Acidsmentioning

confidence: 99%

Application of hydrophilic interaction chromatography in phytochemical analysis

Petruczynik¹,

Waksmundzka‐Hajnos²

2013

Acta Chromatographica

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Summary. Hydrophilic interaction chromatography (HILIC) is a liquid chromatographytechnique when a polar stationary phases -silica or polar bonded phases and aqueous mobile phase containing amount of a less polar solvent (often acetonitrile) is applied. HILIC is important for the separation of highly polar substances including biologically active compounds, such as drugs, amino acids, peptides, proteins, nucleosides, neurotransmitters, etc. HILIC is also the appropriate method for analysis of plant extract polar components such as carbohydrates, amino acids, peptides, phenolic acids, flavonoids and some alkaloids. Plant extracts are usually multicomponent mixtures of compounds of wide polarity range, which often cannot be analyzed by use of a single separation and detection method because of the high chemical diversity. Good results are obtained by use of coupled method, e.g., reversed-phase liquid chromatography (RP-LC) and HILIC mode separation. The elution order in HILIC is usually opposite to that in RP separations. This orthogonality determines one of the advantages of HILIC -the possibility of creating multidimensional separations. An important feature of HILIC is the improved sensitivity with electrospray mass spectrometry. This is significant for the analysis of components existing in small concentration in multicomponent mixtures. The high acetonitrile content also gives additional advantage of HILIC -faster separations due to the lower viscosity of HILIC eluents compared to standard RP ones. The presented review deals with the optimization of HILIC separations and application of the method for analysis of plant extracts components.

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“…12,19 After the ring-opening reaction, the poly(GMA-PEGDMA) monolith possesses diol groups at the surface of the polymer skeleton, which contribute to the hydrophilicity of the column, and the composition of GMA contained in the polymer will affect the hydrophilic properties of the stationary phase. Poly(GMA-PEGDMA) monoliths with different GMA content were prepared and evaluated.…”

Section: Column Characterizationmentioning

confidence: 99%

“…The application of monolithic columns in the HILIC mode has been studied for capillary LC, and it was successfully used for carbohydrates, 6,7 proteins, 8,9 nucleosides, 9,10 phenols and vitamin C, 11 and some small polar analytes. 12 The methacrylate-based polymers are one of the most widely researched monoliths. Methacrylates are the most popular among polymer chemistries used as separation media.…”

Section: Introductionmentioning

confidence: 99%

Methacrylate-based Diol Monolithic Stationary Phase for the Separation of Polar and Non-Polar Compounds in Capillary Liquid Chromatography

2013

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aromatic hydrocarbons (PAHs). PAHs are carcinogenic compounds and exist in a wide range of environmental matrices, and they are normally analyzed with RPLC methods using silica-based C18 packed columns. A monolithic capillary column prepared with glycidyl methacrylate (GMA) and poly(ethylene glycol) dimethacrylate (PEGDMA) was investigated and used in capillary liquid chromatography. The polymer monolith was synthesized in the presence of methanol and decanol as the biporogenic solvents by in situ polymerization of GMA and PEGDMA, and the optimum composition of monomer and porogen was investigated. After polymerization, glycidyl groups were hydrolyzed with sulfuric acid to produce diol groups at the surface of the porous monolith via epoxy-ring-opening. The GMA content in the polymerization mixture affected the hydrophilicity of the monolith. The separation capability was evaluated by separation of phenol compounds, phthalic acids, and polycyclic aromatic hydrocarbons. The poly(GMA-PEGDMA) monolithic capillary column exhibited satisfactory stability.

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Polymethacrylate monolithic and hybrid particle-monolithic columns for reversed-phase and hydrophilic interaction capillary liquid chromatography

Cited by 57 publications

References 27 publications

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

Preparation and characterization of poly-(methacrylatoethyl trimethylammonium chloride-co-vinylbenzyl chloride-co-ethylene dimethacrylate) monolith

Application of hydrophilic interaction chromatography in phytochemical analysis

Methacrylate-based Diol Monolithic Stationary Phase for the Separation of Polar and Non-Polar Compounds in Capillary Liquid Chromatography

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