Polymerization of Affinity Ligands on a Surface for Enhanced Ligand Display and Cell Binding

Richards, Erin; Li, Shihui; Chen, Niancao; Battig, Mark R.; Wang, Yong

doi:10.1021/bm501347s

Cited by 23 publications

(30 citation statements)

References 45 publications

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“…Glass was used as a physical support of the hydrogel films. Glass squares (6 × 6 mm 2 ) were silanized using the procedure as published in the literature . Briefly, glass squares were sonicated in acetone for 15 min, rinsed with deionized water, sonicated in 1 m NaOH for 15 min, and rinsed with deionized water again.…”

Section: Methodsmentioning

confidence: 99%

Development of a Dual‐Functional Hydrogel Using RGD and Anti‐VEGF Aptamer

Zhao

Battig

et al. 2017

Macromolecular Bioscience

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Synthetic molecular libraries hold great potential to advance the biomaterial development. However, little effort is made to integrate molecules with molecular recognition abilities selected from different libraries into a single biomolecular material. The purpose of this work is to incorporate peptides and nucleic acid aptamers into a porous hydrogel to develop a dual-functional biomaterial. The data show that an anti-integrin peptide can promote the attachment and growth of endothelial cells in a 3D porous poly(ethylene glycol) hydrogel and an antivascular endothelial growth factor aptamer can sequester and release VEGF of high bioactivity. Importantly, the dual-functional porous hydrogel enhances the growth and survival of endothelial cells. This work demonstrates that molecules selected from different synthetic libraries can be integrated into one system for the development of novel biomaterials.

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Section: Methodsmentioning

confidence: 99%

Development of a Dual‐Functional Hydrogel Using RGD and Anti‐VEGF Aptamer

Zhao

Battig

et al. 2017

Macromolecular Bioscience

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“…The electrode surface was modified with V 2 O 5 nanobelt, multiwalled carbon nanotubes (MWCNT), and chitosan for electrode surface modification. The electrode was then set as a substrate for immobilization of a single-stranded DNA probe 38 for Yersinia enterocolitica . The assay had a LOD of 1.76 aM and used methylene blue (MB) as an electrochemical redox for labeling the LAMP amplicon.…”

Section: End-point Detectionmentioning

confidence: 99%

Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection

Safavieh

Kanakasabapathy

Tarlan

et al. 2016

ACS Biomater. Sci. Eng.

151

104

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Rapid, sensitive, and selective pathogen detection is of paramount importance in infectious disease diagnosis and treatment monitoring. Currently available diagnostic assays based on polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) are time-consuming, complex, and relatively expensive, thus limiting their utility in resource-limited settings. Loop-mediated isothermal amplification (LAMP) technique has been used extensively in the development of rapid and sensitive diagnostic assays for pathogen detection and nucleic acid analysis and hold great promise for revolutionizing point-of-care molecular diagnostics. Here, we review novel LAMP-based lab-on-a-chip (LOC) diagnostic assays developed for pathogen detection over the past several years. We review various LOC platforms based on their design strategies for pathogen detection and discuss LAMP-based platforms still in development and already in the commercial pipeline. This review is intended as a guide to the use of LAMP techniques in LOC platforms for molecular diagnostics and genomic amplifications.

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“…Later, cell attachment of hydrogel was further enhanced by implementing polyvalent aptamer, in which multiple aptamers were hybridized with one backbone as side units for polyvalency of target cell binding, leading to more cell binding with equal amounts of surface reaction sites. 153, 154 …”

Section: Functional Nucleic Acid-based Hydrogels and Their Bioanalmentioning

confidence: 99%

Functional nucleic acid-based hydrogels for bioanalytical and biomedical applications

et al. 2016

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Hydrogels are crosslinked hydrophilic polymers that can absorb a large amount of water. By their hydrophilic, biocompatible and highly tunable nature, hydrogels can be tailored for applications in bioanalysis and biomedicine. Of particular interest are DNA-based hydrogels owing to the unique features of nucleic acids. Since the discovery of DNA double helical structure, interest in DNA has expanded beyond its genetic role to applications in nanotechnology and materials science. In particular, DNA-based hydrogels present such remarkable features as stability, flexibility, precise programmability, stimuli-responsive DNA conformations, facile synthesis and modification. Moreover, functional nucleic acids (FNAs) have allowed the construction of hydrogels based on aptamers, DNAzymes, i-motif nanostructures, siRNAs and CpG oligodeoxynucleotides to provide additional molecular recognition, catalytic activities and therapeutic potential, making them key players in biological analysis and biomedical applications. To date, a variety of applications have been demonstrated with FNA-based hydrogels, including biosensing, environmental analysis, controlled drug release, cell adhesion and targeted cancer therapy. In this review, we focus on advances in the development of FNA-based hydrogels, which have fully incorporated both the unique features of FNAs and DNA-based hydrogels. We first introduce different strategies for constructing DNA-based hydrogels. Subsequently, various types of FNAs and the most recent developments of FNA-based hydrogels for bioanalytical and biomedical applications are described with some selected examples. Finally, the review provides an insight into the remaining challenges and future perspectives of FNA-based hydrogels.

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Polymerization of Affinity Ligands on a Surface for Enhanced Ligand Display and Cell Binding

Cited by 23 publications

References 45 publications

Development of a Dual‐Functional Hydrogel Using RGD and Anti‐VEGF Aptamer

Development of a Dual‐Functional Hydrogel Using RGD and Anti‐VEGF Aptamer

Emerging Loop-Mediated Isothermal Amplification-Based Microchip and Microdevice Technologies for Nucleic Acid Detection

Functional nucleic acid-based hydrogels for bioanalytical and biomedical applications

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