Polymeric nanoparticles loaded with Baccharis dracunculifolia DC essential oil: Preparation, characterization, and antibacterial activity in milk

Abstract

Eudragit RS100 nanoparticles containing Baccharis dracunculifolia DC essential oil (N‐EO) was developed by the nanoprecipitation method. N‐EO presented an average diameter of 151.6 nm, zeta potential (ζ) of +51.7 mV, and encapsulation efficiency of 99.4%. The antibacterial activity of N‐EO and free essential oil (EO) was evaluated in BHI broth against Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, and Salmonella Enteritidis. No viable cell counts were detected for all strains tested up to 4 hr incubation with EO. The same effect was achieved at 24 hr by the N‐EO, suggesting a controlled release of encapsulated EO. The antimicrobial activity was also tested against L. monocytogenes in milk. Both EO and N‐EO lack antibacterial effect in whole milk, while a delayed lag phase was observed in skimmed milk. The N‐EO caused no significant lysis of erythrocytes suggesting that it can be safe to eukaryotic cells. Practical applications The results of this work suggest that Eudragit nanoparticles can be suitable carriers for essential oils, maintaining their antimicrobial activities. Nanoparticle encapsulation may be useful for food applications by hiding the intense flavor of EO and providing a controlled release of bioactive components. The reduced activity in milk suggests the interaction between the nanostructures and EO with food components, which merits additional studies.

Nanocapsules containing Saussurea lappa essential oil: Formulation, characterization, antidiabetic, anti-cholinesterase and anti-inflammatory potentials

“…The expected benefits from the encapsulation of plant-based products included an enhanced aqueous solubility and bioavailability, a better protection from the environment (pH, temperature, humidity and oxygen) and a persistent delivery (Bilia et al, 2014). Several studies have been dedicated to the encapsulation of plant derived-metabolites such as essential oil (EO) (Chifiriuc et al, 2017;El-Asbahani et al, 2015;Froiio et al, 2019;Herculano et al, 2015;Jummes et al, 2020;Partheniadis et al, 2020;Pina-Barrera et al, 2019;Timbe et al, 2020), vegetable oils (Contri et al, 2013(Contri et al, , 2016Lammari et al, 2020a;Sousa et al, 2013), plants extracts (Azeez et al, 2020;Mughees et al, 2020;Servat-Medina et al, 2015), etc. However, to the best of our knowledge, neither the extract nor the EO of costus roots have been previously encapsulated in polymeric nanoparticles.…”

Development of Fenofibrate/Randomly Methylated β-Cyclodextrin-Loaded Eudragit® RL 100 Nanoparticles for Ocular Delivery

“…In fact, no hemolysis was observed in the two drug-free EuNPs across the same concentration range (10–1000 μg/mL). This indicated that the excipients used in the FE eye drop formulations were not cytotoxic [ 66 , 67 ]. As the FE concentration increased, hemolytic activity increased in a dose-dependent manner.…”

Ethnomedicinal, phytochemical and pharmacological investigations of Baccharis dracunculifolia DC. (ASTERACEAE)

“…Not showing activity against Gram-negative bacteria and yeasts ( Timbé et al, 2021 ). This same research group also evaluated the antimicrobial activity of nanoparticles containing B. dracunculifolia oil ( Harmonia Natural - Canelinha, SC, Brazil) ( Timbé et al, 2020 ). First, the MBC of the oil was analyzed against Listeria monocytogenes (0.56 mg/ml), S. aureus (0.28 mg/ml), B. cereus (0.14 mg/ml) and S. Enteritidis (1.13 mg/ml).…”

“…After 4 h of contact of the essential oil with S. aureus , B. cereus, and L. monocytogenes, and S. Enteritidis, there were no more viable cells. The reduction achieved by the nano encapsulated oil was 2 h of incubation for B. cereus , 10 h for L. monocytogenes, and 24 h for S. aureus and S. Enteritidis, suggesting a gradual release of compounds with antimicrobial activity ( Timbé et al, 2020 ).…”